The disintegrin and metalloprotease Meltrin from Drosophila forms oligomers via its protein binding domain and is regulated by the homeobox protein VND during embryonic development

Meyer, Heiko; Ohlen, Tonia Von; Panz, Mareike; Paululat, Achim

doi:10.1016/j.ibmb.2010.07.010

Cited by 8 publications

(5 citation statements)

References 42 publications

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“…This set of genes is diverse in both the expression domains as well as the pathways in which they function. They include Neu3 ( Meltrin ), which encodes a metalloprotease and is expressed in a pattern largely overlapping with brk , as well as more broadly-expressed genes such as capicua ( cic ) and runt ( run ) which encode transcription factors [24–26]. Even anterior-posterior regulated genes, such as the transcription factor tailless ( tll ) [27], are upregulated, and this change can be seen as tll expression is visibly expanded posteriorly in brkΔ3’ embryos and even more so in the brkΔ5’Δ3’ embryos (Fig 3C, white arrows).…”

Section: Resultsmentioning

confidence: 99%

Coacting enhancers can have complementary functions within gene regulatory networks and promote canalization

2019

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Developmental genes are often regulated by multiple enhancers exhibiting similar spatiotemporal outputs, which are generally considered redundantly acting though few have been studied functionally. Using CRISPR-Cas9, we created deletions of two enhancers, brk5’ and brk3’, that drive similar but not identical expression of the gene brinker (brk) in early Drosophila embryos. Utilizing both in situ hybridization and quantitative mRNA analysis, we investigated the changes in the gene network state caused by the removal of one or both of the early acting enhancers. brk5’ deletion generally phenocopied the gene mutant, including expansion of the BMP ligand decapentaplegic (dpp) as well as inducing variability in amnioserosa tissue cell number suggesting a loss of canalization. In contrast, brk3’ deletion presented unique phenotypes including dorsal expansion of several ventrally expressed genes and a decrease in amnioserosa cell number. Similarly, deletions were made for two enhancers associated with the gene short-gastrulation (sog), sog.int and sog.dist, demonstrating that they also exhibit distinct patterning phenotypes and affect canalization. In summary, this study shows that similar gene expression driven by coacting enhancers can support distinct, and sometimes complementary, functions within gene regulatory networks and, moreover, that phenotypes associated with individual enhancer deletion mutants can provide insight into new gene functions.

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Section: Resultsmentioning

confidence: 99%

Coacting enhancers can have complementary functions within gene regulatory networks and promote canalization

2019

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“…The functions of Drosophila ADAMs are less known than their mammalians counterpart (183). Here we found ADAM meltrin involved in the embryonic central nervous system (184). Drosophila meltrin is the ortholog of human MELTRIN alpha (ADAM 12) involved in cell fusion that leads to the formation of multinucleated cells such as macrophage-derived giant cells (185).…”

Section: Cell Surface and Secreted Enzymes And Inhibitorsmentioning

confidence: 92%

Proteomics of purified lamellocytes from Drosophila melanogaster HopT identifies new membrane proteins and networks involved in their functions

Wan

Belghazi²,

Lemauf³

et al. 2021

Insect Biochemistry and Molecular Biology

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“…Northern blots were generally conducted as described (Meyer et al, 2010) with total RNA (15 g per lane) and a hybridization temperature of 65°C.…”

Section: Western Blot and Northern Blot Analysismentioning

confidence: 99%

Identification of an animal sucrose transporter

Meyer

Vitavska

Wieczorek

2011

Journal of Cell Science

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SummaryAccording to a classic tenet, sugar transport across animal membranes is restricted to monosaccharides. Here, we present the first report of an animal sucrose transporter, SCRT, which we detected in Drosophila melanogaster at each developmental stage. We localized the protein in apical membranes of the late embryonic hindgut as well as in vesicular membranes of ovarian follicle cells. The fact that knockdown of SCRT expression results in significantly increased lethality demonstrates an essential function for the protein.Experiments with Saccharomyces cerevisiae as a heterologous expression system revealed that sucrose is a transported substrate. Because the knockout of SLC45A2, a highly similar protein belonging to the mammalian solute carrier family 45 (SLC45) causes oculocutaneous albinism and because the vesicular structures in which SCRT is located appear to contain melanin, we propose that these organelles are melanosome-like structures and that the transporter is necessary for balancing the osmotic equilibrium during the polymerization process of melanin by the import of a compatible osmolyte. In the hindgut epithelial cells, sucrose might also serve as a compatible osmolyte, but we cannot exclude the possibility that transport of this disaccharide also serves nutritional adequacy.

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The disintegrin and metalloprotease Meltrin from Drosophila forms oligomers via its protein binding domain and is regulated by the homeobox protein VND during embryonic development

Cited by 8 publications

References 42 publications

Coacting enhancers can have complementary functions within gene regulatory networks and promote canalization

Coacting enhancers can have complementary functions within gene regulatory networks and promote canalization

Proteomics of purified lamellocytes from Drosophila melanogaster HopT identifies new membrane proteins and networks involved in their functions

Identification of an animal sucrose transporter

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