The disposition of enrofloxacin in seabream (Sparus aurata L.) after single intravenous injection or from medicated feed administration

Rocca, Giorgia Della; Salvo, Alessandra Di; Malvisi, Jose'; Sello, M

doi:10.1016/s0044-8486(03)00455-1

Cited by 63 publications

(62 citation statements)

References 9 publications

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“…In addition, the susceptible strains of T. maritimum recovered in 2004 showed a marked decline of about 29.2% of the inhibition zone sizes in comparison to the initial values observed in 2003. This is consistent with the relatively high amount of antibiotic therapy applied at the farm during this period, with much higher doses than those recommended for other marine fish species such as seabass (Intorre et al 2000) and seabream (della Rocca et al 2004). Absence of isolation of strains resistant to enrofloxacin from July to August 2004 can be explained by the temporary reduction of the use of this drug.…”

Section: Resultssupporting

confidence: 80%

Evolution of drug resistance and minimum inhibitory concentration to enrofloxacin in Tenacibaculum maritimum strains isolated in fish farms

et al. 2007

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The in vitro susceptibility of 63 isolates of Tenacibaculum maritimum from four fish farms to eight chemotherapeutic agents used for the treatment of bacterial diseases in fish were assessed. The results indicated that all strains were resistant to oxolinic acid and susceptible to amoxicillin, nitrofurantoin, florfenicol, oxytetracycline and trimethoprim-sulphamethoxazole. However, some isolates presented resistance to enrofloxacin and flumequine, ranging from 10 to 30%, and from 25 to 60%, respectively, depending on the farm sampled. These data were used in an attempt to predict whether the resistance to enrofloxacin was static or evolved during the time of sampling from 2003 to 2004. A relationship between the use of enrofloxacin and levels of resistance was detected in the studied farm, increasing significantly from no resistant isolates in 2003 to 44.8% resistant strains in 2004, the year in which this drug was commonly employed. This result was accompanied by a marked decline of about 29.2% of the inhibition zone sizes for the T. maritimum strains in comparison to the initial values (average 21.5 mm). Minimum inhibitory concentration (MIC) of enrofloxacin for 100 T. maritimum strains was determined by the microdilution method. Twenty isolates were resistant to enrofloxacin (> 256 mg ml À1 ), while the remaining strains showed a bimodal distribution, which ranged from 0.5 to 32 mg ml À1 . Our interpretation of the enrofloxacin MIC data suggests that the breakpoint for T. maritimum should be 4 mg ml À1 . However, similar studies in other laboratories are necessary to validate this breakpoint value.

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Section: Resultssupporting

confidence: 80%

Evolution of drug resistance and minimum inhibitory concentration to enrofloxacin in Tenacibaculum maritimum strains isolated in fish farms

et al. 2007

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“…1b). Some microbiological, metabolism and pharmacokinetic aspects of enrofloxacin have been reported from previous studies with fishes (3,4,11,25,26,29,33); however, very few data on enrofloxacin depletion in fishes reared under field conditions are available (11,32).…”

mentioning

confidence: 99%

Long Depletion Time of Enrofloxacin in Rainbow Trout ( Oncorhynchus mykiss )

Lucchetti

Fabrizi

Guandalini

et al. 2004

Antimicrob Agents Chemother

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The international production of farmed fish has been growing continuously over recent years. Until now few veterinary drugs have been approved by the European Union for use in aquaculture, and this has favored the off-label use of products authorized for use in food-producing animal species different from fishes among fish farmers. Adequate field studies are lacking, especially for those species called minor species which are consumed extensively only in some European countries. In the present investigation we studied the depletion of the fluoroquinolone antibacterial enrofloxacin over time in a minor species, the rainbow trout (Oncorhynchus mykiss), reared on a real fish farm and treated with medicated feed (10 mg kg of trout body weight ؊1 day ؊1 ). Edible tissue samples (muscle plus skin in natural proportions) and fish bone samples were analyzed for enrofloxacin and for its major metabolite, ciprofloxacin, by high-performance liquid chromatography with fluorescence detection at different times after the end of treatment. Our results show that at 500°C-day (in which degree-days are calculated by multiplying the mean daily water temperature by the total number of days on which the temperature was measured), which is the minimum withdrawal period established by European Economic Commission Directive No. 82/2001 for any type of product administered off-label, edible trout tissues might still contain about 170 g of enrofloxacin kg ؊1 , whereas the maximum residue level for enrofloxacin plus ciprofloxacin is set at 100 g kg ؊1 . To our knowledge, no studies of the depletion of enrofloxacin in rainbow trout have been performed. On the basis of the data obtained in the present study, we suggest a more appropriate withdrawal time of 816°C-day for the sum of enrofloxacin plus ciprofloxacin levels in rainbow trout muscle plus skin tissues.

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“…[7][8][9] Although several sensitive and accurate analytical methods for the determination of antibiotics in fish tissue can be found in literature, most of them refer either to other antibiotic classes in gilthead, e.g., oxytetracycline and sulfa drugs, [10] or to quinolones, but in other sea-food species. [11][12][13][14][15][16][17][18] Concerning the multiresidue analysis of quinolones in foodproducing gilthead sea bream tissues, there are methods for the analysis of five or less analytes, [19][20][21][22][23][24] except one [25] which mentioned seven quinolones. The majority of the methods use fluorescence for the detection of the examined antibiotics, [5,11,12,16,17,[19][20][21][22][23][24] but other detection techniques like UV [17] and mass spectrometry [5,13,15,18,25] as well as chemiluminescence detection with CeðIVÞÀRuðbpyÞ 3 2þ ÀHNO 3 have been also reported.…”

Section: Introductionmentioning

confidence: 99%

“…[11][12][13][14][15][16][17][18] Concerning the multiresidue analysis of quinolones in foodproducing gilthead sea bream tissues, there are methods for the analysis of five or less analytes, [19][20][21][22][23][24] except one [25] which mentioned seven quinolones. The majority of the methods use fluorescence for the detection of the examined antibiotics, [5,11,12,16,17,[19][20][21][22][23][24] but other detection techniques like UV [17] and mass spectrometry [5,13,15,18,25] as well as chemiluminescence detection with CeðIVÞÀRuðbpyÞ 3 2þ ÀHNO 3 have been also reported. [14] Solid-liquid extraction with different combinations of solvents, such as Residual Quinolones in Fish by LC-DAD 2143 phosphate buffer, citric acid, Na 2 EDTA, dichloromethane, and n-hexane are mainly used [5,6,[19][20][21][22][23][24] with purification by solid phase extraction (SPE) using polymeric or silica-based cartridges as well as immobilized metal chelate affinity chromatographic cleanup.…”

Section: Introductionmentioning

confidence: 99%

“…The majority of the methods use fluorescence for the detection of the examined antibiotics, [5,11,12,16,17,[19][20][21][22][23][24] but other detection techniques like UV [17] and mass spectrometry [5,13,15,18,25] as well as chemiluminescence detection with CeðIVÞÀRuðbpyÞ 3 2þ ÀHNO 3 have been also reported. [14] Solid-liquid extraction with different combinations of solvents, such as Residual Quinolones in Fish by LC-DAD 2143 phosphate buffer, citric acid, Na 2 EDTA, dichloromethane, and n-hexane are mainly used [5,6,[19][20][21][22][23][24] with purification by solid phase extraction (SPE) using polymeric or silica-based cartridges as well as immobilized metal chelate affinity chromatographic cleanup. [11,25] The authors in a previous work [25] had reported on the determination of seven quinolone antibiotics in gilthead sea bream using liquid chromatography-tandem mass spectrometry, which is a rather expensive and not always available analytical technique.…”

Section: Introductionmentioning

confidence: 99%

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Development and Validation of an Lc-Dad Method for the Routine Analysis of Residual Quinolones in Fish Edible Tissue and Fish Feed. Application to Farmed Gilthead Sea Bream Following Dietary Administration

Evaggelopoulou

Samanidou

Michaelidis

et al. 2014

Journal of Liquid Chromatography & Related Technologies

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& The authors in a previous work had reported on the determination of seven quinolone antibiotics in gilthead sea bream using liquid chromatography-tandem mass spectrometry, which is a rather expensive and not always available analytical technique. In this work, photodiode array detection is used, so that no sophisticated instrumentation is necessary, following the same sample preparation procedure. A high performance liquid chromatography method for the determination of seven quinolone antibiotics in fish feed and gilthead sea bream (Sparus aurata L.) tissue was developed and validated in terms of selectivity, linearity, accuracy, precision, sensitivity, robustness, and stability, according to European Decision 2002=657=EC. Ciprofloxacin, danofloxacin, enrofloxacin, sarafloxacin, oxolinic acid, nalidixic acid, and flumequine were separated on a Perfectsil ODS-3 (250 mm Â 4 mm, 5 lm) column by gradient elution, using a mobile phase consisting of 0.1% trifluoroacetic acid (pH ¼ 1), acetonitrile, and methanol at 25 C. Analytes were monitored by diode array detector at 255 nm for acidic quinolones (oxolinic acid, nalidix acid, and flumequine) and 275 nm for ciprofloxacin, danofloxacin, enrofloxacin, and sarafloxacin. The method was successfully applied to gilthead sea bream from aquaculture after dietary administration of flumequine and oxolinic acid.

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The disposition of enrofloxacin in seabream (Sparus aurata L.) after single intravenous injection or from medicated feed administration

Cited by 63 publications

References 9 publications

Evolution of drug resistance and minimum inhibitory concentration to enrofloxacin in Tenacibaculum maritimum strains isolated in fish farms

Evolution of drug resistance and minimum inhibitory concentration to enrofloxacin in Tenacibaculum maritimum strains isolated in fish farms

Long Depletion Time of Enrofloxacin in Rainbow Trout ( Oncorhynchus mykiss )

Development and Validation of an Lc-Dad Method for the Routine Analysis of Residual Quinolones in Fish Edible Tissue and Fish Feed. Application to Farmed Gilthead Sea Bream Following Dietary Administration

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