The distinctive gastric fluid proteome in gastric cancer reveals a multi-biomarker diagnostic profile

Kon, Oi Lian; Yip, Tai‐Tung; Ho, Meng Fatt; Chan, Weng Hoong; Wong, Wai Keong; Tan, Soo-Yong; Ng, Wai Har; Kam, Siok Yuen; Eng, Alvin Kh; Ho, Patrick A.; Viner, Rosa; Ong, Hock Soo; Kumarasinghe, M. Priyanthi

doi:10.1186/1755-8794-1-54

Cited by 38 publications

(40 citation statements)

References 60 publications

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“…Finally, they concluded that the tissue expression of PGC may represent a powerful tool for the diagnosis of prostate cancer. Another report showed that the PGC is among prominent down-regulated markers in the gastric cancer (Kon et al, 2008). At present, there are many reports in support of the biological importance and proteomic signature of PGC as a biomarker for gastric carcinoma (Melle et al, 2005;Kon et al, 2008).…”

Section: Pgc As a Biomarkermentioning

confidence: 98%

“…Another report showed that the PGC is among prominent down-regulated markers in the gastric cancer (Kon et al, 2008). At present, there are many reports in support of the biological importance and proteomic signature of PGC as a biomarker for gastric carcinoma (Melle et al, 2005;Kon et al, 2008). Chae et al (2008) have recently studied the clinical utility of serum pepsinogen levels as a screening test of atrophic gastritis.…”

Section: Pgc As a Biomarkermentioning

confidence: 99%

“…It has been observed that PGC is present in the benign prostatic enlargement and malignant prostate cells. This finding suggests that prostate is the main source of this enzyme and it may be considered as a biomarker of prostate carcinoma (Melle et al, 2005;Kon et al, 2008;Iijima et al, 2009). Interestingly, the presence of PGC in the seminal fluid clearly indicates its potential role in immunofertility (Szecsi and Lilja, 1993).…”

Section: Introductionmentioning

confidence: 99%

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Progastriscin: Structure, Function, and Its Role in Tumor Progression

Hassan

Toor

Ahmad

2010

Journal of Molecular Cell Biology

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Progastricsin (PGC) is a major seminal plasma protein having aspartyl proteinases-like activity and showing close sequence similarity to pepsins. PGC is also present as zymogen in gastric mucosa. In this article, we have reviewed all important features of PGC. Furthermore, we have compared all features of PGC with those of different aspartyl proteinases. The complete amino acid sequence of PGC reveals that it is composed of 374 residues (gastricsin moiety of 331 residues and the activation segment of 43 residues). The gene of human PGC is located at single locus on chromosome 6, whereas the human pepsinogen genetic locus is polymorphic and codes for at least three distinct polypeptide sequences on chromosome 11. The major useful function of PGC includes production of pro-antimicrobial substance in seminal plasma. The crystal structure of human PGC is known, which shows that it is quite similar to that of porcine pepsinogen. The tertiary structure of PGC is comprised of commonly bilobal structure with a large active-site cleft between the lobes. Two aspartate residues in the center of the cleft, namely Asp32 and Asp215, function as catalytic residues. The sequence and structural features of PGC indicate that it is diverged from its pepsinogen ancestor in the early phase of the evolution of gastric aspartyl proteinases. Our detailed review of PGC structure, function and activation mechanism will also be of interest to cancer biologists as well as gastroenterologists.

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Section: Pgc As a Biomarkermentioning

confidence: 98%

Section: Pgc As a Biomarkermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Progastriscin: Structure, Function, and Its Role in Tumor Progression

Hassan

Toor

Ahmad

2010

Journal of Molecular Cell Biology

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“…2. The gastric fluid protein profiles between patients with gastric disease and healthy individuals have been compared using various techniques, such as ProteinChip arrays with copper (II) IMAC3, weak cation exchange (WCX2 and CM10), strong anion exchange (SAX2), and hydrophobic interaction (H50) [35]. In a recent study, clinical gastroscopy, 2-DE, and MS analysis were applied to achieve distinct gastric fluid protein profiles.…”

Section: Gastric Fluidmentioning

confidence: 99%

“…However, the presence of specific gastric fluid biomarkers can further aid in the evaluation of symptomatic patients, thus leading to a definitive diagnosis. Of particular importance are pepsin-related proteins such as pepsinogen C [35], pepsin A [35], α1-antitrypsin [36], and pepsinogen II [37] that have been shown to have altered expressions in gastric cancer. These findings suggest that the activation of proteolytic enzymes in gastric fluid may play an important factor in gastric carcinogenesis.…”

Section: Gastric Fluidmentioning

confidence: 99%

Discovery of biomarkers for gastric cancer: A proteomics approach

Lin

Huang²,

Juan

2012

Journal of Proteomics

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Dynamic Monitoring of Systemic Biomarkers with Gastric Sensors

et al. 2021

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Continuous monitoring in the intensive care setting has transformed the capacity to rapidly respond with interventions for patients in extremis. Noninvasive monitoring has generally been limited to transdermal or intravascular systems coupled to transducers including oxygen saturation or pressure. Here it is hypothesized that gastric fluid (GF) and gases, accessible through nasogastric (NG) tubes, commonly found in intensive care settings, can provide continuous access to a broad range of biomarkers. A broad characterization of biomarkers in swine GF coupled to time‐matched serum is conducted . The relationship and kinetics of GF‐derived analyte level dynamics is established by correlating these to serum levels in an acute renal failure and an inducible stress model performed in swine. The ability to monitor ketone levels and an inhaled anaesthetic agent (isoflurane) in vivo is demonstrated with novel NG‐compatible sensor systems in swine. Gastric access remains a main stay in the care of the critically ill patient, and here the potential is established to harness this establishes route for analyte evaluation for clinical management.

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The distinctive gastric fluid proteome in gastric cancer reveals a multi-biomarker diagnostic profile

Abstract: Background: Overall gastric cancer survival remains poor mainly because there are no reliable methods for identifying highly curable early stage disease. Multi-protein profiling of gastric fluids, obtained from the anatomic site of pathology, could reveal diagnostic proteomic fingerprints.

Cited by 38 publications

References 60 publications

Progastriscin: Structure, Function, and Its Role in Tumor Progression

Progastriscin: Structure, Function, and Its Role in Tumor Progression

Discovery of biomarkers for gastric cancer: A proteomics approach

Dynamic Monitoring of Systemic Biomarkers with Gastric Sensors

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