The distribution and changes of glycoalkaloids in potato tubers under different storage time based on MALDI-TOF mass spectrometry imaging

Deng, Yamei; He, Muyi; Feng, Feng; Feng, Xuesong; Zhang, Yuan; Zhang, Feng

doi:10.1016/j.talanta.2020.121453

Cited by 39 publications

(30 citation statements)

References 39 publications

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“…Alkaloid intake in small quantities can lead to gastrointestinal symptoms, such as abdominal pain, vomiting, and diarrhea, whereas higher alkaloid doses can cause acute poisoning or severe symptoms, such as coma, neurological disorders, cardiac failure, and paralysis (Mensinga et al, 2005 ). Our results showed that ClO 2 could improve the antioxidant capacity and the safety of potato tubers by promoting the accumulation of flavonoids and inhibiting the synthesis of alkaloids, respectively (Deng et al, 2020 ; Wang et al, 2020 ).…”

Section: Discussionmentioning

confidence: 90%

Integrated Analysis of Transcriptome and Metabolome Reveals the Mechanism of Chlorine Dioxide Repressed Potato (Solanum tuberosum L.) Tuber Sprouting

Zheng

Tian

et al. 2022

Front. Plant Sci.

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Sprouting is an irreversible deterioration of potato quality, which not only causes loss in their commercial value but also produces harmful toxins. As a popular disinfectant, ClO2 can inhibit the sprouting of potato tubers. Using transcriptomic and metabolomic approaches to understand the repressive mechanism of ClO2 in potato sprouting is yet to be reported. Sequencing the transcriptome and metabolome of potatoes treated with ClO2 in this study revealed a total of 3,119 differentially expressed genes, with 1,247 and 1,872 genes showing down- and upregulated expression, respectively. The majority of the downregulated genes were associated with plant hormone signal transduction, whereas upregulated differential genes were associated primarily with biological processes, such as phenylpropanoid biosynthesis and the mitogen-activated protein kinase (MAPK) signaling pathway. Metabonomic assays identified a total of 932 metabolites, with 33 and 52 metabolites being down- and upregulated, respectively. Downregulated metabolites were mostly alkaloids, amino acids, and their derivatives, whereas upregulated metabolites were composed mainly of flavonoids and coumarins. Integrated transcriptomic and metabolomic analyses showed that many different metabolites were regulated by several different genes, forming a complex regulatory network. These results provide new insights for understanding the mechanism of ClO2-mediated repression of potato sprouting.

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Section: Discussionmentioning

confidence: 90%

Integrated Analysis of Transcriptome and Metabolome Reveals the Mechanism of Chlorine Dioxide Repressed Potato (Solanum tuberosum L.) Tuber Sprouting

Zheng

Tian

et al. 2022

Front. Plant Sci.

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“…Finally, in potato tubers, toxic glycoalkaloids, e.g., dehydrosolanine, α-solanine, dehydrochaconine, and α-chaconine, were shown to accumulate upon storage in the sprout and the periderm but less in the medulla. The highest levels were detected in the sprout [51].…”

Section: Spatial Localization Of Plant Metabolitesmentioning

confidence: 95%

Unravel the Local Complexity of Biological Environments by MALDI Mass Spectrometry Imaging

Sgobba

Daguerre

Giampà

2021

IJMS

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Classic metabolomic methods have proven to be very useful to study functional biology and variation in the chemical composition of different tissues. However, they do not provide any information in terms of spatial localization within fine structures. Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) does and reaches at best a spatial resolution of 0.25 μm depending on the laser setup, making it a very powerful tool to analyze the local complexity of biological samples at the cellular level. Here, we intend to give an overview of the diversity of the molecules and localizations analyzed using this method as well as to update on the latest adaptations made to circumvent the complexity of samples. MALDI MSI has been widely used in medical sciences and is now developing in research areas as diverse as entomology, microbiology, plant biology, and plant–microbe interactions, the rhizobia symbiosis being the most exhaustively described so far. Those are the fields of interest on which we will focus to demonstrate MALDI MSI strengths in characterizing the spatial distributions of metabolites, lipids, and peptides in relation to biological questions.

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“…Methods based on LC-MS/MS are commonly used for targeted analysis of GAs, while methods using LC-HRMS, mainly with Orbitrap technology, are more frequently used for metabolomic studies and to detect unknown compounds. To reduce interferences of complex matrices efficient sample pretreatment methods including ultrasonic extraction, pressurized liquid extraction and SPE have been used [ 12 , 138 ].…”

Section: Glycoalkaloidsmentioning

confidence: 99%

Overview of Recent Liquid Chromatography Mass Spectrometry-Based Methods for Natural Toxins Detection in Food Products

Girolamo

Lippolis

Pascale

2022

Toxins

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Natural toxins include a wide range of toxic metabolites also occurring in food and products, thus representing a risk for consumer health. In the last few decades, several robust and sensitive analytical methods able to determine their occurrence in food have been developed. Liquid chromatography mass spectrometry is the most powerful tool for the simultaneous detection of these toxins due to its advantages in terms of sensitivity and selectivity. A comprehensive review on the most relevant papers on methods based on liquid chromatography mass spectrometry for the analysis of mycotoxins, alkaloids, marine toxins, glycoalkaloids, cyanogenic glycosides and furocoumarins in food is reported herein. Specifically, a literature search from 2011 to 2021 was carried out, selecting a total of 96 papers. Different approaches to sample preparation, chromatographic separation and detection mode are discussed. Particular attention is given to the analytical performance characteristics obtained in the validation process and the relevant application to real samples.

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The distribution and changes of glycoalkaloids in potato tubers under different storage time based on MALDI-TOF mass spectrometry imaging

Cited by 39 publications

References 39 publications

Integrated Analysis of Transcriptome and Metabolome Reveals the Mechanism of Chlorine Dioxide Repressed Potato (Solanum tuberosum L.) Tuber Sprouting

Integrated Analysis of Transcriptome and Metabolome Reveals the Mechanism of Chlorine Dioxide Repressed Potato (Solanum tuberosum L.) Tuber Sprouting

Unravel the Local Complexity of Biological Environments by MALDI Mass Spectrometry Imaging

Overview of Recent Liquid Chromatography Mass Spectrometry-Based Methods for Natural Toxins Detection in Food Products

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