The distribution and density of monocarboxylate transporter 2 in cerebral cortex, hippocampus and cerebellum of wild‐type mice

Abstract

Monocarboxylates cannot cross the blood‐brain barrier freely to participate in brain energy metabolism. Specific monocarboxylate transporters (MCTs) are needed to cross cellular membranes. Monocarboxylate transporter 2 (MCT2) is a major monocarboxylate transporter encoded by the SLC16A7 gene. Recent studies reported that neurodegenerative diseases of the CNS, such as Alzheimer's disease (AD) and Parkinson's disease (PD), were related to energy metabolic impairment. MCT2 also plays an important role in energy metabolism in the CNS. To provide experimental evidence for future research on the role of MCT2 in the pathological process of CNS degenerative diseases, the distribution and density of MCT2 in different subregions of wild‐type mouse brain was examined using immunohistochemistry, western blot and immunogold post‐embedding electron microscopic techniques. The amount of MCT2 was higher in cerebellum than in cortex and hippocampus on western blots, and there was no statistical difference between cortex and hippocampus. Immunohistochemistry assay revealed the highest density of MCT2 in the CA3 of the hippocampus. The granular cell layer of the cerebellum contained more MCT2 than the molecular layer. The MCT2 density on the end feet of astrocytes of molecular layer was lower than in hippocampus, but the postsynaptic densities (PSDs) of asymmetric synapses in the molecular layer exhibited a high density using immunogold post‐embedding electron microscopic techniques.

MCT2 overexpression promotes recovery of cognitive function by increasing mitochondrial biogenesis in a rat model of stroke

“…Furthermore, cognitive deficits may be due, in part, to impaired energy metabolism in the brain. Our finding that MCT2 expression was reduced in the rat brain after stroke is consistent with a previous study that found MCT2 expression was decreased in patients with AD (Pang et al 2020 ). In our study, reduced MCT2 expression was accompanied by an increase in brain lactate levels.…”

Distribution of Cadherin in the Parahippocampal Area of Developing Domestic Chicken Embryos

“…To investigate the possibility by using cadherins as markers to explore structural homology, we compared the expression of cadherin mRNAs in the hippocampal formation and parahippocampal areas of chicken and mice. Since the structure of the chicken telencephalon is relatively mature at 16 embryonic days, we chose the E18 chicken embryo and adult mouse brain sections He Zhou, et al [31,32]. The V-shaped region was proposed to be homologous to the dentate gyrus, the DM region (approximately corresponding to the APHm and APHi) was proposed to be homologous to Ammon' s horn and the subiculum in mammals, and the DL region (approximately corresponding to the APHl) was proposed to be homologous to the entorhinal cortex [10,12].…”

Treadmill exercise improves brain energy metabolism, motor, and cognitive functions in Hypoxic Ischemic Encephalopathy mice model

“…By severing unilateral or bilateral common carotid arteries, the normal blood supply to the brain is affected. Whether there is a connection between brain injury and energy metabolism and whether it can improve the energy supply of brain cells by promoting the utilization of lactic acid in the brain need to be con rmed in this experiment [17,18]. Based on the above assumptions, we hypothesized that the normal glucose energy metabolism is blocked, and more lactic acid is used as the energy substrate in the HIE mice model.…”