The distribution and prognostic implications of steroid receptors in endometrial carcinomas

Utaaker, Edle; Iversen, Ole Erik; Skaarland, E

doi:10.1016/s0090-8258(87)80013-6

Cited by 30 publications

(10 citation statements)

References 23 publications

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“…Comparisons of reports should, be made with caution, however, because criteria and control of aneuploidy, as well as technique and cut-off values for the receptor analyses, may differ. In our experience, steroid receptors were more often detectable in endometrial carcinomas and found at higher values than in ovarian carcinomas (6,13). To predict the course of the disease, we chose a dividing point of receptor values of 30 fmol mglprotein.…”

Section: Discussionmentioning

confidence: 99%

“…Receptor or ploidy status did not influence treatment decisions. The cytosol estradiol (ER) and progesterone receptors (PR) were measured by the single saturation dose (SSD) technique as described in detail elsewhere (6,13). The detection limits for the assays were calculated on the basis of the variation in non-specific binding (95% confidence interval), and found to be 5 fmol/mg protein for ER and 10 fmol/rng protein for PR.…”

Section: Dna Ploidymentioning

confidence: 99%

“…I NTRO D U CTI 0 N Steroid receptor status in tumor tissue has been shown to be of value in predicting both the course of the disease and the response to endocrine treatment (1)(2)(3)(4)(5)(6) in patients with breast and endometrial carcinoma. In a recent study of endometrial carcinoma by Creasman et al (l), the prognostic information given by receptor status for disease-free survival was found to be independent of previously known clinical and pathological factors.…”

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confidence: 99%

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DNA ploidy and steroid receptors as predictors of disease course in patients with endometrial carcinoma

Iversen

Utaaker

Skaarland

1988

Acta Obstet Gynecol Scand

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Prognostic factors for outcome of malignant disease should be based on objective assessments whenever possible, so that the results may be reproduced. In a prospective study, tumor samples from 75 patients were subjected to flow cytometric DNA analysis. Samples were also taken from 61 patients for estradiol and progesterone receptor measurements. The course of the disease was analysed with regard to ploidy and receptor status. Receptor status was significantly correlated with ploidy, as diploid tumors were more often receptor-positive or receptor-rich (greater than or equal to 30 fmol/mg protein). Mortality and recurrence rates were highest among patients with aneuploid or receptor-poor tumors. Ploidy, receptor status, histological grade, surgical stage, and myometrial invasion were found to be of significant prognostic value. By multivariate analysis, ploidy was indicated to be the best predictor, followed by surgical stage. DNA and receptor measurements are recommended in research on endometrial carcinoma, and may become useful in routine clinical work.

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Section: Discussionmentioning

confidence: 99%

Section: Dna Ploidymentioning

confidence: 99%

mentioning

confidence: 99%

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DNA ploidy and steroid receptors as predictors of disease course in patients with endometrial carcinoma

Iversen

Utaaker

Skaarland

1988

Acta Obstet Gynecol Scand

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“…Several studies, mainly biochemical, have established an inverse correlation between ER and PR contents and the histologic differentiation of endometrial adenocarcinoma, showing that poorly differentiated adenocarcinomas are more often receptor "negative" (Creasman et al 198.5, Budwit-Novotny et al 1986, Geisinger et al 1986, Liao et al 1986, Mutch et al 1987Ehrlich et al 1988, Segreti et al 1989, Cargangiu et al 1990, Kleine et al 1990, Nyholm et al 1990) and when receptor "positive", they display lower amounts of ER and PR than do moderately and well-differentiated tumors, differences between the latter two often being less pronounced (Kauppila et al 1982a, Creasman et al 1985, Quinn et al, 198.5a, Liao et al 1986, Utaaker et al 1987Chambers et al 1988, Palmer et al 1988, Kleine et al 1990, Nyholm et al 1992 (Table 11).…”

Section: Receptor Correlations With Histologic Gradementioning

confidence: 99%

Estrogen and progesterone receptors in endometrial cancer Clinicopathological correlations and prognostic significance

Nyholm¹

1996

APMIS

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“…Human endometrium expresses ER and PR, and both are present in carcinomas, whether assessed by biochemical or immunohistochemical assays (Evans et al 1975, Carcangiu et al 1990, Chambers et al 1990). ER and PR content in endometrial carcinoma are important prognostic indicators for survival, which correlate with histological stage and grade (McCarty et al 1979, Martin et al 1979, Hunter et al 1980, Kauppila et al 1980, 1982, Ehrlich et al 1981, Martin et al 1983, Vihko et al 1983, Mortel et al 1984, Creasman et al 1985, Geisinger et al 1986, Liao et al 1986, Utaaker et al 1987, Chambers et al 1988. It therefore seems possible that the response to hormonal therapy may be related to tumour receptor content.…”

Section: Introductionmentioning

confidence: 99%

Multiple isoforms of the oestrogen receptor in endometrial cancer

Marsigliante¹,

Muscella²,

Ciardo³

et al. 1995

Journal of Molecular Endocrinology

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We evaluated the presence and variability of oestrogen receptor (ER) isoforms in endometrial cancer by using [3H]oestradiol-labelled ERs and the H222 monoclonal antibody obtained from the Abbott enzyme immunoassay kit. Using isoelectric focusing (IEF), endometrial ER was shown to be composed of four different species, with pI values of 6.1, 6.3, 6.6 and 6.8, indistinguishable from the isoforms found in normal rat uterus, and human breast and larynx carcinomas. The isoforms at pI 6.3, 6.6 and 6.8, all sedimenting at 4S by sucrose gradient fractionation, showed, on two-dimensional SDS electrophoresis, relative masses of 50, 70 and 65 kDa respectively, equal to the masses previously found in breast cancer. These isoforms did not alter their pI values during IEF fractionation performed in a linear gradient of urea, while the pI 6.1, sedimenting at 8S, generated a new isoform at about 9 mol/l urea with pI 7.2 and a relative mass of 65 kDa. The urea-dissociated isoform (pI 7.2) was able approximately to double the antibody binding with respect to the nondissociated oligomer, which suggested that some epitopes are 'masked', i.e. not accessible to the antibodies when ER is present in its complexed form. The evidence thus suggested that the oligomer at pI 6.1 contained a single 65 kDa ER form which, as a monomer, focused at pI 7.2. The variability in the ER isoform profile found in endometrial cancer was similar to the variability previously reported in breast and larynx carcinomas. The balance between these isoforms could be a dynamic parameter involved in the functionality of this receptor and consequently in cell transformation.

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The distribution and prognostic implications of steroid receptors in endometrial carcinomas

Cited by 30 publications

References 23 publications

DNA ploidy and steroid receptors as predictors of disease course in patients with endometrial carcinoma

DNA ploidy and steroid receptors as predictors of disease course in patients with endometrial carcinoma

Estrogen and progesterone receptors in endometrial cancer Clinicopathological correlations and prognostic significance

Multiple isoforms of the oestrogen receptor in endometrial cancer

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