The Distribution of Alkaline Phosphatase in Normal and Pathologic Human Skin

Kopf, Alfred W.

doi:10.1001/archderm.1957.01550130003001

Cited by 85 publications

(22 citation statements)

References 122 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…(6) Alkaline phosphatase (A1P) Contrary to K opf [7] and Braun-Falco [2] but in agreement with Wolff [11] and Kint [6], none of our cases showed an intratum oral AlPase activity, except for the walls of the superficial capillaries present in the tumor strands. The distribution of APe-ase activity did not show a constant pattern: in some cases the highest activity was found in the outer parts of the tumors, whereas in other cases the enzyme activity was noted mainly in the central parts.…”

supporting

confidence: 89%

Histochemical Observations in Skin Cancer, Keratoacanthoma, and Some Other Tumors of the Skin

Suurmond¹

1968

Dermatology

View full text Add to dashboard Cite

supporting

confidence: 89%

Histochemical Observations in Skin Cancer, Keratoacanthoma, and Some Other Tumors of the Skin

Suurmond¹

1968

Dermatology

View full text Add to dashboard Cite

“…Previous reports indicated strong alkaline phosphatase presence in DP cells throughout the hair follicle cycle and expression in the lower portions of the DS of anagen-stage hair follicles has been claimed by some (Johnson et al, 1945;Butcher, 1951;Hardy, 1952;Kopf, 1957;Braun-Falco, 1958;Handjiski et al, 1994). This consistent expression of alkaline phosphatase in DP cells has been utilized as a simple and reliable method to distinguish dermisderived cells of the DP from other hair follicle structures throughout the hair follicle cycle .…”

Section: Resultsmentioning

confidence: 87%

Cultured Peribulbar Dermal Sheath Cells Can Induce Hair Follicle Development and Contribute to the Dermal Sheath and Dermal Papilla

McElwee

Kissling

Wenzel

et al. 2003

Journal of Investigative Dermatology

224

218

View full text Add to dashboard Cite

Green fluorescent protein (GFP)-expressing wild-type, and nontransgenic mouse vibrissa follicle cells were cultured and implanted to mouse ears and footpads. Dermal papiller (DP)-derived cells and cells from the peribulbar dermal sheath "cup" (DSC) induced new hair follicles in both implanted ears and footpads, while nonbulbar dermal sheath cells did not. Confocal microscopy revealed that GFP-expressing DP and DSC cells induced hair growth associated with the formation of DP exclusively comprised of fluorescent cells. In mouse ears, but not footpads, fluorescent DP and DSC cells could also be identified in DP along with nonfluorescent cells. DSC cells were characterized in vivo and in vitro by low alkaline phosphatase activity in contrast to high alkaline phosphatase in DP cells. The results indicate transplanted DP and DSC cells were equally capable of DP formation and hair follicle induction. This suggests the DP and peribulbar DSC may be functionally similar. In addition to observing papillae exclusively composed of GFP-expressing cells, DP and DSC cells may also have combined with resident cells to form papillae composed of implanted GFP-expressing cells and host-derived non-GFP-expressing cells. Alkaline phosphatase expression may be utilized as a simple marker to identify hair follicle mesenchyme derived cells with hair follicle inductive abilities.

show abstract

“…They have been studied by cytochemical and the electron microscopic techniques in salivary glands (24,(40)(41)(42)(44)(45)(46); in lacrimal glands (25,26,40); in mammary glands (5,11,15,22,42,44); in the prostate (38); in the Harderian gland (4, 25); and in apocrine) (14,16,19,48) and eccrine (8,13,18,30,32,33 sweat glands. All of these investigators agree that the myoepithelial cells lie on the epithelial side of the basement membrane, and most authors note the similarity between filaments in the cytoplasm of smooth muscle and myoepithelium.…”

Section: Introductionmentioning

confidence: 99%

Fine Structure of the Myoepithelium of the Eccrine Sweat Glands of Man

Ellis

1965

The Journal of Cell Biology

115

View full text Add to dashboard Cite

A B S T R A C TThe secretory coils of glutaraldehyde-osmium tetroxide-fixed and Epon-Araldite-embedded eccrine sweat glands from the palms of young men were studied with the electron microscope. The myoepithclial cells lie on the epithelial side of the basement membrane and abut other epithelial elements directly. The irregularly serrated base of the cell has dense thickenings along the plasma membranc which alternate with zones bcaring pits; the smooth apical surface lacks dense thickenings, is studded with pits, and conjoined to secretory cells by cccasional desmosomes. Masses of myofilaments, 50 A in diameter, fill most of the cell and are associated with irregular dense zones. In cross-section the arrangement of the myofilaments seems identical with that of the I band of striated muscle, and the dense zone has typical Z band structure. A few microtubules and cytoplasmic cores bearing profiles of the endoplasmic reticulum, filamentous mitochondria, and glycogen granules penetrate the fibrillar masses and run parallel to the oriented myofilaments. In the perinuclear zone, Golgi membranes, rough-and smooth-surfaced elements of the endoplasmic reticulum, mitochondria, glycogen, microtubules, lipid, pigment, and dense granules are variable components in the cytoplasm. The interrelationships of the myoepithelial cells with the secretory cells suggest that the former may act as regulators, controlling the flow of metabolites to the secretory epithelium. I N T R O D U C T I O NMyoepithelial cells are common components of many vertebrate glands that take their origin from the embryonic ectoderm. They have been studied by cytochemical and the electron microscopic techniques in salivary glands (24,(40)(41)(42)(44)(45)(46); in lacrimal glands (25,26,40); in mammary glands (5,11,15,22,42,44); in the prostate (38); in the Harderian gland (4, 25); and in apocrine) (14,16,19,48) and eccrine (8,13,18,30,32,33 sweat glands. All of these investigators agree that the myoepithelial cells lie on the epithelial side of the basement membrane, and most authors note the similarity between filaments in the cytoplasm of smooth muscle and myoepithelium. The literature shows that although the myoepithelial cells of various glands may differ in size and shape, they are essentially similar in their fine structure. In some glands the contractile function of the myoepithelial elements has been clearly demonstrated (16,27,46) and the similarities in fine structure between the myoepithelial elements of the different glands suggest that they all are contractile cells.In studies seeking the elements basic to all intracellular contractile systems, the organization of every type of contractile cell should be considered. In this respect, striated muscle has been investigated intensively and some progress is now being made in elucidating the structure and function of smooth muscle (21, 34), but for lack of information or from oversight the myoepithelial cell has often 551

show abstract

The Distribution of Alkaline Phosphatase in Normal and Pathologic Human Skin

Cited by 85 publications

References 122 publications

Histochemical Observations in Skin Cancer, Keratoacanthoma, and Some Other Tumors of the Skin

Histochemical Observations in Skin Cancer, Keratoacanthoma, and Some Other Tumors of the Skin

Cultured Peribulbar Dermal Sheath Cells Can Induce Hair Follicle Development and Contribute to the Dermal Sheath and Dermal Papilla

Fine Structure of the Myoepithelium of the Eccrine Sweat Glands of Man

Contact Info

Product

Resources

About