The Distribution of Alkaline Phosphatase in Various Tissues

Abstract

1. Further details of the staining of various organs by Gomori's phosphatase technique have been given. 2. A modification of the technique which involves adding sodium alizarin sulphonate to the substrate mixture has been described. 3. A description has been given of the distribution of phosphatase in regenerating bone. 4. It has been found that phosphatase does not appear to be associated with the calcification of the hen's egg. 5. Phosphatase appears to play some part in the secretion of the shell of certain molluscs. 6. The intensity of phosphatase staining of kidney and adrenal was found to be undiminished in scurvy, but the periosteum and regenerating bone were found to stain less intensely than normal in scorbutic animals.

Studies on the fractionation of mucosal homogenates from the small intestine

“…The view of a localization of alkaline phosphatase in more than one subcellular structure is supported by the results of histochemical studies. Ever since Gomori (1941) described a phosphatase activity in the surface of human small intestine, many workers have either confirmed this observation or extended it to other tissues including the epithelial layer of the intestinal mucosa of the mouse (Deane & Dempsey, 1945;Brandes, Zetterqvist & Sheldon, 1956), the guinea pig (Bourne, 1943;Jervis, 1963), the rat (Emmel, 1945;Johnson & Kugler, 1953;Jervis, 1963), the rabbit (Jervis, 1963) and the cat and dog (Johnson & Kugler, 1953). In most of these investigations it was pointed out that alkaline-phosphatase activity was seen in bilaminar layers of the brush border as well as in the apical region of the cytoplasm, which in some instances was referred to as the Golgi zone.…”

Relationship of Human Salivary Alkaline Phosphatase With Abo Blood Group and Secretor Status

“…Although much of the evidence concerning the histochemical localization of salivary alkaline phosphatase comes from other mammals, there is little doubt that the enzyme is, in fact, synthesized by the human salivary glands (dock, Murray and Pincus, 1938;Bourne, 1943;Deane, 1947;Noback and Montagna, 1947;Newman, Feigin, Wolf and Kabat, 1950;Leeson, 1956;Arvy, 1963). However, it is probable that part of the phosphatase activity of saliva is derived from cellular debris and oral microorganisms (dock et al, 1938;Lura, 1947;Chauncey, personal communication).…”

Acid and Alkaline Phosphatases in Normal and Diseased Human Muscle

“…in cases of facio-seapulohum eral or fam ilial d y stro p h y , th ere were a few fine bu t scattered connective tissue fibres w ith alkaline phosphatase ac tiv ity . They were probably new ly form ed fibres since these have been shown (Bourne [1943], Fell and Danielli 119431) to contain the enzym e.…”