The distribution of chromatin in budding yeast cells

Lindegren, Carl C.; Williams, Marion A.; McClary, Dan O.

doi:10.1007/bf02538308

Cited by 37 publications

(23 citation statements)

References 24 publications

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“…In our previous studies (1-3) of vegetatively dividing cells of Saccharomyces cerevisiae, we sought to exploit the superior resolution obtainable with electron microscopy of ultrathin sections. The results were difficult to reconcile with earlier reports (4,5), based on light microscopy, which had pictured classical mitotic figures and distinct chromosome structures. Rather, we concluded that vegetative nuclear division is characterized by (a) the absence of distinct chromosomes of the type seen in higher organisms, (b) the persistence of the nuclear mere-*Present address: Department of Bacteriology, Tokushima University Medical School, Tokushima, Japan.…”

Section: Introductioncontrasting

confidence: 95%

Studies on the Fine Structure of Microorganisms

Hashimoto

Conti

Naylor

1960

The Journal of Cell Biology

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The fine structure of cells of Saccharomyces cerevisiae engaged in the formation of ascospores was studied in electron micrograpbs of ultrathin sections. Although the mode of the first reduction division could not be clearly determined, the second nuclear division appeared to proceed in a manner similar to that observed previously during vegetative division. That is, division by constriction of the existing nucleus occurs without dissolution of the nuclear membrane and without involvement of discrete chromosomes. Variously shaped areas of low electron density were discerned within the nucleoplasm; these had not been previously seen in the vegetative nucleus. The significance of this nuclear differentiation and its possible similarity to nuclear structures reported in bacteria and an imperfect fungus are discussed.The cytoplasmic membrane appears first in the developing ascospore. The formation of an outer coat and an inner coat then follows. The cytoplasmic vacuole was observed not to be incorporated into the spore. An unusual intracytoplasmic membrane was observed in the spore and appeared to be at least temporarily continuous with the nuclear membrane. I N T R O D U C T I O NDisagreement has existed and still persists on the pattern of nuclear division in both vegetative and sporulating yeast cells. In our previous studies (1-3) of vegetatively dividing cells of Saccharomyces cerevisiae, we sought to exploit the superior resolution obtainable with electron microscopy of ultrathin sections. The results were difficult to reconcile with earlier reports (4, 5), based on light microscopy, which had pictured classical mitotic figures and distinct chromosome structures. Rather, we concluded that vegetative nuclear division is characterized by (a) the absence of distinct chromosomes of the type seen in higher organisms, (b) the persistence of the nuclear mere-*Present address:

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Section: Introductioncontrasting

confidence: 95%

Studies on the Fine Structure of Microorganisms

Hashimoto

Conti

Naylor

1960

The Journal of Cell Biology

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“…It is a generally accepted view that the vacuole is not an integral part of the nuclear apparatus of yeasts as had been proposed by Lindegren et al (1956). Previous studies by Hashimoto et al (1958 and, Naylor (1959, 1960a, and 1960b), Vitols et al (1961), and Thyagarajan et al (1962) have led to a similar conclusion.…”

Section: Structure Of C Albic Ans 101mentioning

confidence: 67%

STUDIES ON THE FINE STRUCTURE OF CANDIDA ALBICANS, WITH SPECIAL REFERENCE TO INTRACYTOPLASMIC MEMBRANE SYSTEM

Takagi

Nagata

1962

Japanese Journal of Microbiology

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“…The nucleus in resting ascospores. A (listinet morphological entity with the vegetative cell of S. cerevisiae has been referred to as the nucleus by some workers (Hawthorne, 1955; Agar and Douglas, 1957) and as the spindle by Lindegren et al (1956). A similar structure (N) in all figures will be referred to as the nucleus in this presentation.…”

Section: Resultsmentioning

confidence: 99%