The Distribution of Heptose and 2-Keto-3-deoxy-octonate in Bacteroidaceae

Hofstad, Tor

doi:10.1099/00221287-85-2-314

Cited by 70 publications

(25 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Desiccated LPS rapidly increased in weight (by approximately 15 %) when the sample was exposed to the air. Heptose was not detected by the cysteine-sulphuric acid method (Dische, 1953), as modified by Wright & Rebers (1972), or with aniline hydrogen phthalate after separating the sugars by paper chromatography (Hofstad, 1974). The same LPS composition was obtained when the bacteria were grown slowly in 0.5 or 2-5 1 batches in rotating flasks.…”

Section: Analysis Of Iipopolysaccharidementioning

confidence: 65%

Properties of Phage-receptor Lipopolysaccharide from Pseudomonas morsprunorum

Quirk

Sletten

Hignett

1976

Journal of General Microbiology

View full text Add to dashboard Cite

S U M M A R YLipopolysaccharide (LPS) of Pseudomonas morsprunorum was extracted with hot phenol and purified by repeated centrifuging followed by either block electrophoresis or gel filtration. LPS from a virulent isolate exhbited specific phage inactivation (PIso = 0.05 ,ug LPS ml-l), whereas LPS from an avirulent phageresistant mutant did not. LPS was considered pure when a single band was detected following sodium dodecyl sulphate-cellulose acetate electrophoresis (PH 7-4). It was not phytotoxic when inoculated into cherry leaves at concentrations up to I mgml-l, but produced weak chlorosis in bean and tobacco at 2 mg ml-l : no visible symptoms appeared after treatment with lower concentrations. The chemical composition of the LPS was partly determined. I N T R O D U C T I O NThere is a strong correlation between phage sensitivity and host specificity in the English isolates of Pseudomonas morsprunorum, the cause of cherry canker (Crosse & Garrett, 1970). Isolates from cherry are sensitive to A7 and related phages (Crosse & Garrett, 1963) and infect cherry trees (Prunus avium) through the leaf scars. Almost all plum strains are insensitive to these phages and are non-virulent through cherry leaf scars. Plum strains adsorb phage A7 as readily as do cherry strains, suggesting that phage-receptor material in the bacterial wall is not involved in the host specificity of the strains (Garrett, Crosse & Sletten, I 974).Cherry-strain mutants resistant to these phages show marked attenuation of virulence. Their resistance is due to an inability to adsorb the phage, indicating a change in the receptor-site structure (Garrett et al., 1974). Hence, receptor-site material may determine the virulence of P. morsprunorum as plum and cherry isolates adsorb the phage and are both virulent on their respective hosts.Lipopolysaccharide (LPS) of Gram-negative bacterial walls has frequently been reported as phage-receptor material (e.g. Jazwinski, Lindberg & Kornberg, 1975) and also as an endotoxin (e.g. Hawiger, Hawiger & Timmon, 1975). In view of this, lipopolysaccharide was extracted from P. morsprunorum to investigate its role in pathogenicity and as receptorsite material for phage A7 and related phages. pH 71 to which one drop of antifoam (polypropylene glycol 2000; Shell Research, Sittingbourne, Kent) was added before autoclaving. The cultures were stirred and aerated at 18 "C. Air was supplied at 500 cm3 min-l via a sintered-glass gas distribution tube (porosity 3). The purity of the cultures was tested by the Gram-stain method and by subculturing on nutrient sucrose agar (NSA; Crosse, 1959). The optimum temperature for growth of c28 is normally 25 "C but when grown in broth with efficient aeration and stirring at this temperature, cells grew to 4 to 5 times their normal length. These cells quickly reverted to the normal size when aeration was reduced and were not elongated when grown at 18 "C. Cells were harvested at the late-exponential phase using a continuous-flow centrifuge at 4 "C, and washed twice with cold distilled ...

show abstract

Section: Analysis Of Iipopolysaccharidementioning

confidence: 65%

Properties of Phage-receptor Lipopolysaccharide from Pseudomonas morsprunorum

Quirk

Sletten

Hignett

1976

Journal of General Microbiology

View full text Add to dashboard Cite

show abstract

“…Cell wall peptidoglycan may contain either lysine or diaminopimelic acid. No heptose or 2-keto-3-deoxyoctulosonic acid is detected (9). Cells contain sphingolipids (25) and have superoxide dismutase activity (8).…”

Section: Resultsmentioning

confidence: 99%

Description of Bacteroides loescheii sp. nov. and Emendation of the Descriptions of Bacteroides melaninogenicus (Oliver and Wherry) Roy and Kelly 1939 and Bacteroides denticola Shah and Collins 1981

Holdeman¹,

Johnson²

1982

International Journal of Systematic Bacteriology

View full text Add to dashboard Cite

“…LPS of B. gingivalis, B. intermedius and B..fragilis are basically similar and possess no significant quantities of heptose and KDO (Hofstad, 1974;Johne & Bryn, 1986;Koga et al, 1985;Mansheim et al, 1978). All the LPS preparations from the Bacteroides species used in this study possess various quantities of hexose, glucosamine, fatty acid and phosphorus, but no detectable heptose or KDO (results not shown).…”

Section: Discussionmentioning

confidence: 99%

“…The chemical composition and some biological properties of the LPS from oral and non-oral Bacteroides species have been reported by several groups, demonstrating that both blackpigmented and non-black-pigmented Bacteroides species possess rather unusual LPS in terms of their chemical compositions and biological activities (Hofstad, 1974;Mansheim et al, 1978 ;Koga et al, 1985). However, no comparative examination of immunobiological properties of the various LPS from different species and strains of black-pigment forming Bacteroides of oral origin has been attempted.…”

Section: T F U J I W a R A A N D Othersmentioning

confidence: 99%

Serological Properties and Immunobiological Activities of Lipopolysaccharides from Black-pigmented and Related Oral Bacteroides Species

Fujiwara

Nishihara²,

Kinoshita³

et al. 1988

Microbiology

View full text Add to dashboard Cite

Lipopolysaccharides (LPS) from five species of oral Bacteroides, B. gingivalis strains 38 1 and ATCC 33277, B. oralis ATCC 33269, B. 1oescheiiATCC 15930, B. intermedius ATCC 2561 1 and B. corporis ATCC 33547, were extracted from whole cells by the phenol/water procedure, and subsequently purified by treatment with nuclease and ultracentrifugation. The LPS were composed of hexoses, glucosamine, fatty acids and phosphorus. Heptose and 2-keto-3-deoxyoctonate were not detected. The LPS preparations from B. gingivalis strains 381 and ATCC 33277 presented very similar SDS-polyacrylamide gel electrophoresis patterns when stained with ammoniacal silver. They produced a fused precipitin band against an antiserum to B. gingivalis 381 LPS in immunodiffusion tests. Antisera raised against the LPS from B. loescheii and B. intermedius reacted with the LPS prepared from all the oral Bacteroides strains except those of B. gingivalis. All the LPS preparations were mitogenic for spleen cells of BALB/c (nulnu) mice, but not for thymus cells from C3H/HeN mice. The LPS induced marked mitogenic responses and polyclonal B cell activation for spleen cells of not only C3H/HeN (LPS responder) mice, but also C3H/HeJ (LPS nonresponder) mice. The mitogenic responses were not suppressed significantly upon addition of polymyxin B to the reaction mixture. These LPS also enhanced interleukin-1 production by murine peritoneal macrophages and mouse cell line 5744

show abstract

The Distribution of Heptose and 2-Keto-3-deoxy-octonate in Bacteroidaceae

Cited by 70 publications

References 25 publications

Properties of Phage-receptor Lipopolysaccharide from Pseudomonas morsprunorum

Properties of Phage-receptor Lipopolysaccharide from Pseudomonas morsprunorum

Description of Bacteroides loescheii sp. nov. and Emendation of the Descriptions of Bacteroides melaninogenicus (Oliver and Wherry) Roy and Kelly 1939 and Bacteroides denticola Shah and Collins 1981

Serological Properties and Immunobiological Activities of Lipopolysaccharides from Black-pigmented and Related Oral Bacteroides Species

Contact Info

Product

Resources

About