The distribution of inhibitory and excitatory synapses on single, reconstructed jaw-opening motoneurons in the cat

Shigenaga, Yoshio; Moritani, Masayuki; Oh, Soo Jin; Park, K.P.; Paik, Sang Kyoo; Bae, Jin Young; Kim, H.N.; Ma, Songhua; Park, C.W.; Yoshida, Atsushi; Ottersen, Ole Petter; Bae, Yong Chul

doi:10.1016/j.neuroscience.2005.02.022

Cited by 31 publications

(35 citation statements)

References 58 publications

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“…The staining pattern in the rat JO motoneurons was similar to that in our previous studies of JC and JO motoneurons in the rat and the cat (Bae et al, 1999(Bae et al, , 2002Shigenaga et al, 2005;Paik et al, 2007Paik et al, , 2011. Omission or replacement of the primary sera with normal rabbit serum or preadsorption of the diluted anti-GABA serum with 200 lM GABA-G, the anti-Gly serum with 300 lM Gly-G, and the anti-Glut serum with 300 lM Glut-G abolished the immunostaining.…”

Section: Antibody Characterizationsupporting

confidence: 85%

“…Animals were anesthetized with sodium pentobarbital (40 mg/kg, i.p. ), and the anterior belly of the digastric muscle, which is one of major JO muscle and has been used as a representative JO muscle in a large number of experiments on trigeminal motor system Lund and Olsson, 1983;Bae et al, 2002;Shigenaga et al, 2005), was exposed through a small incision of the overlying skin. In total 2-5 ll horseradish peroxidase (HRP, type IV, 10% in saline; Toyobo, Tokyo, Japan) was carefully injected into the muscle via a 30-G needle glued to a Hamilton microsyringe.…”

Section: Retrograde Labeling Of Jaw-opening Motoneuronsmentioning

confidence: 99%

“…To evaluate the immunoreactivity for Glut, we compared the gold particle density (number of gold particles/lm 2 ) over each bouton in contact with a soma of a HRP-labeled JO motoneuron with the average tissue density in 15-30 randomly selected areas of 2 lm 2 each (total area 30-60 lm 2 ) per section; the areas adjacent to JO motoneurons somata and apposing boutons were selected. Boutons containing gold particles at a density >2.576 SD of the average tissue density (99% confidence level) were considered Glut immunopositive (Shigenaga et al, 2005;Paik et al, 2011). To assess the immunoreactivity for GABA and Gly, the gold particle density of each bouton was compared with that of its postsynaptic somatic compartment.…”

Section: Quantitative Analysismentioning

confidence: 99%

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Development of γ‐aminobutyric acid‐, glycine‐, and glutamate‐immunopositive boutons on rat jaw‐opening motoneurons

Paik

Kwak

Bae

et al. 2012

J of Comparative Neurology

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Inhibitory and excitatory synaptic inputs onto trigeminal motoneurons play an important role in coordinating jaw movements. Previously, we reported that the phenotype of the inhibitory boutons apposing the somata of jaw-closing (JC) motoneurons changes from γ-aminobutyric acid (GABA)-positive (GABA+) to predominantly glycine-positive (Gly+) during development. In the present study, we investigated the development of inhibitory and excitatory boutons apposing antagonistic jaw-opening (JO) motoneurons (anterior digastric motoneurons) at postnatal day 2 (P2), P11, and P31 in the rat. JO motoneurons were retrogradely labeled with horseradish peroxidase. Postembedding immunogold staining with antisera against GABA, Gly, and glutamate (Glut) was performed and followed by quantitative ultrastructural analysis. The size of both small and large JO motoneurons increased during development. The number of excitatory (Glut+) and inhibitory (GABA+, Gly+, and GABA+/Gly+) boutons per JO motoneuron increased significantly from P2 to P11 and then remained unchanged until P31. The time course of inhibitory synapse formation differed between JO and JC motoneurons, whereas that of excitatory synapse formation was similar between the two neuronal populations. The fraction of GABA+ boutons decreased by 86% and the fraction of GABA+/Gly+ boutons increased by 200% from P11 to P31, suggesting a switch from GABA+ to GABA+/Gly+ phenotype. The fraction of Gly+ boutons remained unchanged. These results indicate that inhibitory synapses onto somata of JO motoneurons exhibit a developmental pattern distinct from that of synapses onto JC motoneurons, which may reflect distinctive maturation of oral motor system.

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Section: Antibody Characterizationsupporting

confidence: 85%

Section: Retrograde Labeling Of Jaw-opening Motoneuronsmentioning

confidence: 99%

Section: Quantitative Analysismentioning

confidence: 99%

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Development of γ‐aminobutyric acid‐, glycine‐, and glutamate‐immunopositive boutons on rat jaw‐opening motoneurons

Paik

Kwak

Bae

et al. 2012

J of Comparative Neurology

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“…Apparently, MMNs and DMNs receive GABAergic inputs that are weaker than glycinergic inputs from the SupV. The number of glycine-immunoreactive boutons synapsing on MMNs and DMNs in cats is close to twice the number of GABA-immunoreactive boutons (Bae et al 1999;Shigenaga et al 2005). The SupV may be the origin of glycinergic and GABAergic axon terminals at MMNs and DMNs.…”

Section: Glycinergic and Gabaergic Inputs From The Supv To The Movmentioning

confidence: 95%

Synaptic Transmission From the Supratrigeminal Region to Jaw-Closing and Jaw-Opening Motoneurons in Developing Rats

Nakamura

Inoue²,

Nakajima³

et al. 2008

Journal of Neurophysiology

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“…These results indicate that the input-output gain of some SupV premotor neurons decreases during postnatal development. Jaw-closing motoneurons have large and well-developed dendrites, which receive ascending and descending inputs 33,34 . Thus, it is possible that dendritic properties of the jaw-closing motoneurons also change with increasing postnatal age, and we investigated this hypothesis during early development in rats 35 .…”

Section: Postnatal Development Of Synaptic Transmission From Premotormentioning

confidence: 99%

Central Neural Mechanisms Involved in the Control of Jaw Movement during Postnatal Development

Nakamura

Nagata

Nonaka

et al. 2017

Showa Univ J Med Sci

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: Mechanisms responsible for controlling masticatory muscle activity during suckling and mastication initiate in the brain. Premotor neurons situated in the brainstem transmit masticatory motor commands to the jaw-closing and jaw-opening motoneurons, which in turn activate the masticatory muscles. The premotor neurons receive inputs from multiple sources such as the orofacial sensory organs, the cerebral cortex, and the central pattern generator in the brainstem. These neural circuits might be important for controlling jaw movement, although their properties in this regard are likely to be altered in postnatal development as the oral motor behavior of mammals shifts significantly from suckling to chewing during the early postnatal period. This timing of jaw movement development mimics that of the orofacial musculoskeletal apparatus. The current article reviews ndings on local neural circuits of trigeminal motoneurons and premotor neurons, during early development in rats. We describe findings from electrophysiological recordings, optical imaging with voltage-sensitive dyes, calcium imaging, and laser photolysis of caged glutamate. We also review research into the subtypes of premotor neurons in the supratrigeminal region SupV that differ in their electrophysiological properties and possess different morphological characteristics. We further describe how trigeminal motoneurons receive glutamatergic, glycinergic, and GABAergic inputs from the SupV and the reticular formation dorsal to the facial nucleus. Finally, we discuss how these synaptic and intrinsic membrane properties change in postnatal development. Taken together, the ndings reviewed herein suggest that these neural circuits are important in diverse oral motor behaviors, and that their postnatal changes are involved in the transition from suckling to mastication functions.

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The distribution of inhibitory and excitatory synapses on single, reconstructed jaw-opening motoneurons in the cat

Cited by 31 publications

References 58 publications

Development of γ‐aminobutyric acid‐, glycine‐, and glutamate‐immunopositive boutons on rat jaw‐opening motoneurons

Development of γ‐aminobutyric acid‐, glycine‐, and glutamate‐immunopositive boutons on rat jaw‐opening motoneurons

Synaptic Transmission From the Supratrigeminal Region to Jaw-Closing and Jaw-Opening Motoneurons in Developing Rats

Central Neural Mechanisms Involved in the Control of Jaw Movement during Postnatal Development

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