The Distribution of Mucins, Carcinoembryonic Antigen, and Mucus-Associated Antigens in Endocervical and Endometrial Adenocarcinomas

Maes, Gert; Fleuren, Gert Jan; Bara, Jacques; Nap, Marius

doi:10.1097/00004347-198805000-00002

Cited by 22 publications

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“…1). As 45M1 reacts strongly with the mucin from both rat, mouse and human [24], our results suggest that the 45M1 epitope is associated with such a sequence alone or that it is discontinuous and built up of parts found in both this sequence and more C‐terminal ones.…”

Section: Resultsmentioning

confidence: 84%

Mapping of the 45M1 epitope to the C‐terminal cysteine‐rich part of the human MUC5AC mucin

2007

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Mucins are large glycoproteins protecting mucosal surfaces throughout the body. Their expressions are tissue‐specific, but in disease states such as cystic fibrosis, inflammation and cancer, this specificity can be disturbed. MUC5AC is normally expressed in the mucous cells of the epithelia lining the stomach and the trachea, where it constitutes a major component of the gastric and respiratory mucus. A number of mAbs have been raised against the gastric M1 antigen, an early marker for colonic carcinogenesis. Several of these mAbs recognize epitopes present on MUC5AC, suggesting that MUC5AC is the antigen. However, some of the mAbs raised against the gastric M1 antigen are widely used as antibodies against MUC5AC, despite the fact that their specificity for MUC5AC has not been clearly shown. In this study, we have tested the reactivity of the latter antibodies against a recombinantly expressed C‐terminal cysteine‐rich part of human MUC5AC. We demonstrate for the first time that the widely used mAb 45M1, as well as 2‐12M1 and 166M1, are true antibodies against MUC5AC, with epitopes located in the C‐terminal cysteine‐rich part of the mucin.

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Section: Resultsmentioning

confidence: 84%

Mapping of the 45M1 epitope to the C‐terminal cysteine‐rich part of the human MUC5AC mucin

2007

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“…The earliest identified immunophenotypic difference between cervical and endometrial adenocarcinoma was the increased frequency and intensity of CEA immunoreactivity in cervical adenocarcinomas [5], an observation since confirmed in multiple studies [4,7,15,17]. These studies have given a wide range of results, with 50-100% of cervical adenocarcinomas reported to be CEA positive, compared with 0-50% of endometrial adenocarcinoma studies [4,5,7,15,17].…”

Section: Discussionmentioning

confidence: 99%

Immunoprofile of cervical and endometrial adenocarcinomas using a tissue microarray

et al. 2003

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Adenocarcinomas of the uterine cervix show a wide range of morphological features, and can be confused with endometrial adenocarcinoma in biopsy or curetting specimens. The objective of this study was to use tissue microarray technology to evaluate the immunoprofile of a large set of uterine adenocarcinomas with an extended panel of antibodies, comparing the profile of primary cervical and endometrial adenocarcinomas. A tissue microarray was constructed using paraffin-embedded, formalin-fixed tissues from 141 hysterectomy specimens. Duplicate 0.6-mm cores were obtained from 57 cervical adenocarcinomas (16 in situ and 41 invasive) and 84 endometrial adenocarcinomas. Tissue array sections were immunostained with 21 commercially available antibodies [B72.3, CD 99, carcinoembryonic antigen (CEA), c-kit, pancytokeratin, CK 5/ 6, CK 7, CK8/18, CK19, CK 20, CK 22, EMA, estrogen receptor (ER), KP-1, melan-A, p53, PLAP, S-100, synaptophysin, TTF-1, and vimentin] utilizing the avidin-biotin (ABC) technique. Hierarchical clustering analysis of the tumors was done based on the immunostaining results. Only ER (P<0.001), CEA (P=0.04), vimentin (P<0.001), and CK 8/18 (P=0.002) showed a significantly different frequency of positivity in endometrial relative to cervical adenocarcinomas. ER, vimentin, and CK 8/18 were more likely to be expressed in endometrial adenocarcinomas, while cervical adenocarcinomas more fre-quently expressed CEA. We were able to identify immunoprofiles that were highly specific for endocervical adenocarcinoma (ER , vimentin , CK 8/18 , CEA + ) or endometrial adenocarcinoma (ER + , vimentin + , CK 8/18 + , CEA ), but most tumors showed an intermediate, nonspecific immunophenotype. Hierarchical clustering analysis was useful in the interpretation of these intermediate immunophenotypes. Papillary serous adenocarcinoma of the endometrium was less likely to express vimentin (P=0.002) than endometrioid carcinoma of the endometrium.

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Endometrial Carcinoma

Mazur

Kurman

2005

Diagnosis of Endometrial Biopsies and Curettings

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The Distribution of Mucins, Carcinoembryonic Antigen, and Mucus-Associated Antigens in Endocervical and Endometrial Adenocarcinomas

Cited by 22 publications

References 0 publications

Mapping of the 45M1 epitope to the C‐terminal cysteine‐rich part of the human MUC5AC mucin

Mapping of the 45M1 epitope to the C‐terminal cysteine‐rich part of the human MUC5AC mucin

Immunoprofile of cervical and endometrial adenocarcinomas using a tissue microarray

Endometrial Carcinoma

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