The Distribution of Phosphatidylcholine Species in Superficial-Type Pharyngeal Carcinoma

Abstract

Objectives. Superficial-type pharyngeal squamous cell carcinoma (STPSCC) is defined as carcinoma in situ or microinvasive squamous cell carcinoma without invasion to the muscular layer. An exploration of the biological characteristics of STPSCC could uncover the invasion mechanism of this carcinoma. Phosphatidylcholine (PC) in combination with fatty acids is considered to play an important role in cell motility. Imaging mass spectrometry (IMS) is especially suitable for phospholipid analysis because this technique can distinguish even fatty acid compositions. Study Design. IMS analysis of frozen human specimens. Methods. IMS analysis was conducted to elucidate the distribution of PC species in STPSCC tissues. STPSCC tissue sections from five patients were analyzed, and we identified the signals that showed significant increases in the subepithelial invasive region relative to the superficial region. Results. Three kinds of PC species containing arachidonic acid, that is, PC (16:0/20:4), PC (18:1/20:4), and PC (18:0/20:4), were increased in the subepithelial invasive region. Conclusion. These results may be associated with the invasion mechanism of hypopharyngeal carcinoma.

Short communication: Distribution of phospholipids in parotid cancer by matrix-assisted laser desorption/ionization imaging mass spectrometry

“…This suggests that parotid cancer tissue has a markedly different phospholipid composition compared to that of non-tumor tissue. Alterations in the lipid composition of tissues have been reported in several cancers, including breast cancer [ 18 , 21 ], prostate cancer [ 17 , 19 ], lung cancer [ 22 ], kidney cancer [ 23 ], pharyngeal cancer [ 24 ], and oral cancer [ 20 , 25 , 26 ]. Furthermore, it has been suggested that these specific lipid profiles vary from among carcinomas, and may be potential diagnostic, prognostic, and predictive biomarkers.…”

Transferosomes as nanocarriers for drugs across the skin: Quality by design from lab to industrial scale

“…Most transferosomes contain phosphatidylcholine (C18) as the lipid fraction (usually above 70%) as it is the most abundant lipid component of the cell membrane, and hence, it is highly tolerated for the skin, decreasing the risk of undesirable effects, such as hypersensitivity reactions (Ishikawa et al, 2017). Soya phosphatidylcholine (>95 % purity) is a GRAS (Generally Regarded As Safe) excipient and actually it has been used in suspensions, emulsions, mixed micelles, solid dispersions and drug-phospholipid complexes with a good safety profile (Rajan et al, 2011;van Hoogevest and Wendel, 2014).…”