The diversity and abundance of bacterial and fungal communities in the rhizosphere of Cathaya argyrophylla are affected by soil physicochemical properties

Abstract

Cathaya argyrophylla is an ancient Pinaceae species endemic to China that is listed on the IUCN Red List. Although C. argyrophylla is an ectomycorrhizal plant, the relationship between its rhizospheric soil microbial community and soil properties related to the natural habitat remains unknown. High-throughput sequencing of bacterial 16S rRNA genes and fungal ITS region sequences was used to survey the C. argyrophylla soil community at four natural spatially distributed points in Hunan Province, China, and functional profiles were predicted using PICRUSt2 and FUNGuild. The dominant bacterial phyla included Proteobacteria, Acidobacteria, Actinobacteria, and Chloroflexi, and the dominant genus was Acidothermus. The dominant fungal phyla were Basidiomycota and Ascomycota, while Russula was the dominant genus. Soil properties were the main factors leading to changes in rhizosphere soil bacterial and fungal communities, with nitrogen being the main driver of changes in soil microbial communities. The metabolic capacities of the microbial communities were predicted to identify differences in their functional profiles, including amino acid transport and metabolism, energy production and conversion, and the presence of fungi, including saprotrophs and symbiotrophs. These findings illuminate the soil microbial ecology of C. argyrophylla, and provide a scientific basis for screening rhizosphere microorganisms that are suitable for vegetation restoration and reconstruction for this important threatened species.

The Composition and Function of the Rhizosphere Bacterial Community of Paeonia lactiflora Varies with the Cultivar

“…The results of this study showed that Actinobacteria, Proteobacteria, Acidobacteria, Bacteroidetes, Firmicutes, Verrucomicrobia, Planctomycetes and Chloroflexi were the dominant phyla in the microbiomes of P. lactiflora cultivars, among which the relative abundance of Actinobacteria, Proteobacteria and Acidobacteria was much higher than that of other phyla. This finding has also been confirmed in Cathaya argyrophylla and Chinese fir [48,49]. We also noted that the rhizosphere bacterial communities associated with P. lactiflora exhibited high diversity and abundance.…”

Exploring the Impact of Coconut Peat and Vermiculite on the Rhizosphere Microbiome of Pre-Basic Seed Potatoes under Soilless Cultivation Conditions

“…Ascomycota and Basidiomycota , the predominant fungal phyla, are present in all groups, accounting for over 90% of the microbial communities [ 187 , 207 , 208 , 209 ]. Demonstrating strong adaptability, these phyla are widely distributed across various environments, such as soil, rocks, and organic matter, thus dominating microbial communities.…”

Interkingdom multi-omics analysis reveals the effects of nitrogen application on growth and rhizosphere microbial community of Tartary buckwheat

“…Using 20 ng/μL of the DNA extracted from the rhizosphere soil of YQ1, YQ2, XQ2 and FH as PCR template. The bacterial 16s rRNA V3-V4 region specific primers (338F: 5′-ACTCCTCCTACGGGAGCAGCAGCAMUR-3′; 806R: 5′-GGACTACHVGGGTWTCTAAT-3′) were used for PCR amplification ( Xie et al, 2023 ). The reaction system was as follows: Q5 high-fidelity DNA polymerase (0.25 μL), 5 × reaction buffer (5 μL), 5 × high GC buffer (5 μL), dNTP (10 mM, 2 μL), template DNA (2 μL), forward primer (10 μM, 1 μL), reverse primer (10 μM, 1 μL) and ddH 2 O (8.75 μL).…”

“…A total of 20 ng/μL of DNA extracted from YQ1, YQ2, XQ2 and FH rhizosphere soil was also used as template for PCR amplification of fungal ITS sequence. The fungal ITS1 region primers (ITS5-1737-F: 5′-GGAAGTAAAAGTCGTAACAAGG-3′; ITS2-2043-R: 5′-GCTGCGTTCTTCATCGATGC-3′) were selected for amplification and sequencing ( Xie et al, 2023 ). The PCR reaction system was the same as the above 16S rDNA high-throughput sequencing.…”