The Diversity of Cultivable Hydrocarbon-Degrading Bacteria Isolated From Crude Oil Contaminated Soil and Sludge From Arzew Refinery in Algeria

Abstract

The use of autochtonious bacterial strains is a valuable bioremediation strategy for cleaning the environment from hydrocarbon pollutants. The isolation, selection and identification of hydrocarbon-degrading bacteria is therefore crucial for obtaining the most promising strains for decontaminate a specific site. In this study, two different media, a minimal medium supplemented with petroleum and with oil refinery sludge as sole carbon source, were used for the isolation of native hydrocarbon-degrading bacterial strains from crude oil contaminated soils and oil refinery sludges which allowed isolation of fifty-eight strains.The evalution of diversity of twenty-two bacterials isolates reveled a dominance of the phylum Proteobacteria (20/22 strains), with a unique class of Alphaproteobacteria, the two remaining strains belong to the phylum Actinobacteria. Partial 16S rRNA gene sequencing performed on isolates showed high level of identity with known sequences. Strains were affiliated to Sinorhizobium, Promicromonospora, Novosphingobium, Georgenia, Ancylobacter, Roseomonas, Hansschlegelia and Tistrella genera. Research for the genes that encoding for degradation enzymes in isolated genera genome data deposited in Genbank reveled the presence of degradation gene in three species Sinorhizobium meliloti, Novosphingobium panipatenseand Tistrella mobilis.

Current Concentrations of Zn, Cu, and As in Piggery Wastewater Compromise Nutrient Removals in Microalgae–Bacteria Photobioreactors Due to Altered Microbial Communities

“…Among them, the genus Tistrella stood out for its abundance in the R1-control (more than 40% during phase II) but also for its decrease in the presence of Zn and Cu without recovery during phase III. Information on this genus is scarce, although some authors have indicated the potential of some strains, isolated from soils and wastewater, to produce and degrade polycyclic aromatic hydrocarbons [ 45 , 46 ]. More recently, SEM analysis showed Tistrella sp.…”