The diversity of hydrogen-producing bacteria and methanogens within an in situ coal seam

Su, Xinming; Zhao, Weizhong; Xia, Daping

doi:10.1186/s13068-018-1237-2

Cited by 108 publications

(66 citation statements)

References 71 publications

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“…Methanogenesis is commonly associated with sulfate-poor biodegraded petroleum reservoirs 51 and initial steps of anaerobic utilization of organic matter (fermentation) involve hydrolysis followed by bacterial acetogenesis that converts volatile fatty acids into acetic acid, H 2 and CO 2 52 . Alternatively, H 2 is produced by aromatization of compounds present in the seep oil 51 .…”

Section: Discussionmentioning

confidence: 99%

Timing and origin of natural gas accumulation in the Siljan impact structure, Sweden

et al. 2019

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Fractured rocks of impact craters may be suitable hosts for deep microbial communities on Earth and potentially other terrestrial planets, yet direct evidence remains elusive. Here, we present a study of the largest crater of Europe, the Devonian Siljan structure, showing that impact structures can be important unexplored hosts for long-term deep microbial activity. Secondary carbonate minerals dated to 80 ± 5 to 22 ± 3 million years, and thus postdating the impact by more than 300 million years, have isotopic signatures revealing both microbial methanogenesis and anaerobic oxidation of methane in the bedrock. Hydrocarbons mobilized from matured shale source rocks were utilized by subsurface microorganisms, leading to accumulation of microbial methane mixed with a thermogenic and possibly a minor abiotic gas fraction beneath a sedimentary cap rock at the crater rim. These new insights into crater hosted gas accumulation and microbial activity have implications for understanding the astrobiological consequences of impacts.

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Section: Discussionmentioning

confidence: 99%

Timing and origin of natural gas accumulation in the Siljan impact structure, Sweden

et al. 2019

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“…The majority of the previous studies on indigenous microbial community structures of coal and formation water were focused on biogenic or thermogenic methane-containing coal mines [21, 23, 44, 45]. This study provides direct evidence that there are diverse potential microbial coal degraders and methanogens inhabiting opencast coal mine environment, and microbial communities cultured from the grassland soil covering the coal or surrounding the coal mine can efficiently convert the coal into methane with a maximum yield rate reached 74.67 μmol/g coal, which is quite consistent with the methanogenic rate from lignite in the laboratory [20].…”

Section: Discussionmentioning

confidence: 99%

“…The dominant bacterial phyla include Spirochetes , Firmicutes , Proteobacteria and Bacteroidetes [20]. Furthermore, these dominant bacterial phyla and methanogens have been often detected in both biogenic and non-biogenic coalbed methane fields [21]. We et al reported that the microbial communities in thermogenic gas coal mines exhibited potential for biogenic methanogenesis [22].…”

Section: Introductionmentioning

confidence: 99%

Bioconversion of coal to methane by microbial communities from soil and from an opencast mine in the Xilingol grassland of northeast China

Wang

Cui

et al. 2019

Biotechnol Biofuels

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Background The Xilingol grassland ecosystem has abundant superficial coal reserves. Opencast coal mining and burning of coal for electricity have caused a series of environmental challenges. Biogenic generation of methane from coal possesses the potential to improve economic and environmental outcomes of clean coal utilization. However, whether the microbes inhabiting the grassland soil have the functional potential to convert coal into biomethane is still unclear. Results Microbial communities in an opencast coal mine and in grassland soil covering and surrounding this mine and their biomethane production potential were investigated by Hiseq sequencing and anaerobic cultivation. The microbial communities in covering soil showed high similarity to those in the surrounding soil, according to the pairwise weighted UniFrac distances matrix. The majority of bacterial communities in coal and soil samples belonged to the phyla Firmicutes, Bacteroidetes, Actinobacteria and Proteobacteria. The dominant bacterial genera in grassland soil included Gaiella, Solirubrobacter, Sphingomonas and Streptomyces; whereas, the most abundant genus in coal was Pseudarthrobacter. In soil, hydrogenotrophic Methanobacterium was the dominant methanogen, and this methanogen, along with acetoclastic Methanosarcina and methylotrophic Methanomassiliicoccus, was detected in coal. Network-like Venn diagram showed that an average of 28.7% of microbial communities in the samples belonged to shared genera, indicating that there is considerable microbial overlap between coal and soil samples. Potential degraders and methanogens in the soil efficiently stimulated methane formation from coal samples by the culturing-based approach. The maximum biogenic methane yields from coal degradation by the microbial community cultured from grassland soil reached 22.4 μmol after 28 day. Conclusion The potential microbial coal degraders and methanogenic archaea in grassland soil were highly diverse. Significant amounts of biomethane were generated from coal by the addition of grassland soil microbial communities. The unique species present in grassland soil may contribute to efficient methanogenic coal bioconversion. This discovery not only contributes to a better understanding of global microbial biodiversity in coal mine environments, but also makes a contribution to our knowledge of the synthetic microbiology with regard to effective methanogenic microbial consortia for coal degradation.

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“…For instance, strains of Enterobacter Raoultella and Klebsiella have been associated with H 2 and biogas formation (through the generation of acetate), in bioreactors fed with wastewater or GLYC [38,39,40]. The presence of Enterobacter and Citrobacter in biogas formation has also been reported elsewhere [41,42].…”

Section: Resultsmentioning

confidence: 95%

Characterisation and microbial community analysis of lipid utilising microorganisms for biogas formation

et al. 2019

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In the anaerobic process, fat-oil-grease (FOG) is hydrolysed to long-chain fatty acids (LCFAs) and glycerol (GLYC), which are then used as substrates to produce biogas. The increase in FOG and LCFAs inhibits methanogenesis, and so far, most work investigating this inhibition has been carried out when FOG or LCFAs were used as co-substrates. In the current work, the inhibition of methanogenesis by FOG, LCFAs and GLYC was investigated when used as sole substrates. To gain more insight on the dynamics of this process, the change of microbial community was analysed using 16S rRNA gene amplicon sequencing. The results indicate that, as the concentrations of cooking olive oil (CO, which represents FOG) and LCFAs increase, methanogenesis is inhibited. For instance, at 0.01 g. L-1 of FOG, the rate of biogas formation was around 8 ml.L-1.day-1, and this decreased to <4 ml.L-1.day-1 at 40 g.L-1. Similar results were observed with the use of LCFAs. However, GLYC concentrations up to 100g.L-1 did not affect the rate of biogas formation. Acidic pH, temperature > = 45°C and NaCl > 3% led to a significant decrease in the rate of biogas formation. Microbial community analyses were carried out from samples from 3 different bioreactors (CO, OLEI and GLYC), on day 1, 5 and 15. In each bioreactor, microbial communities were dominated by Proteobacteria, Firmicutes and Bacteroidetes phyla. The most important families were Enterobacteriaceae, Pseudomonadaceae and Shewanellaceae (Proteobacteria phylum), Clostridiacea and Ruminococcaceae (Firmicutes) and Porphyromonadaceae and Bacteroidaceae (Bacteroidetes). In CO bioreactor, Proteobacteria bacteria decreased over time, while those of OLEI and GLYC bioreactors increased. A more pronounced increase in Bacteroidetes and Firmicutes were observed in CO bioreactor. The methanogenic archaea Methanobacteriaceae and Methanocorpusculaceae were identified. This analysis has shown that a set of microbial population is selected as a function of the substrate.

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The diversity of hydrogen-producing bacteria and methanogens within an in situ coal seam

Cited by 108 publications

References 71 publications

Timing and origin of natural gas accumulation in the Siljan impact structure, Sweden

Timing and origin of natural gas accumulation in the Siljan impact structure, Sweden

Bioconversion of coal to methane by microbial communities from soil and from an opencast mine in the Xilingol grassland of northeast China

Characterisation and microbial community analysis of lipid utilising microorganisms for biogas formation

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