The DLVO theory in microbial adhesion

Hermansson, Malte

doi:10.1016/s0927-7765(99)00029-6

Cited by 862 publications

(570 citation statements)

References 78 publications

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“…DLVO theory describes microbial adhesion as a balance between attractive Lifshitz-Van der Waals and repulsive or attractive electrostatic forces (Hermansson, 1999). Using this theory the microorganisms can be calculated to be located in the so-called secondary interaction minimum, at approximately 3 nm from the surface under the current conditions (Bos et al, 1999;Rutter, 1980).…”

Section: Methodsmentioning

confidence: 99%

“…Biofilm formation on biomaterial implant surfaces and subsequent infectious complications are a frequent reason for failure of many biomedical devices, such as total hip arthroplasties, in-dwelling voice prostheses and vascular and urinary catheters (Gristina, 1987). While the mechanism of bacterial adhesion to the biomaterial surface has not been fully elucidated, interactions between biomaterial surfaces and bacteria have been reported to include non-specific and specific interactions, such as electrostatic and Lifshitz-Van der Waals forces, hydrophobic interactions and a variety of specific receptor-adhesin interactions (Hermansson, 1999;Busscher & Weerkamp, 1987).…”

Section: Introductionmentioning

confidence: 99%

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Inhibition of adhesion of yeasts and bacteria by poly(ethylene oxide)-brushes on glass in a parallel plate flow chamber

et al. 2003

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Poly(ethylene oxide) (PEO)-brushes are generally recognized as protein-repellent surfaces, and although a role in discouraging microbial adhesion has been established for some strains and species, no study exists on the effects of PEO-brushes on a large variety of bacterial and yeast strains. In this paper, a PEO-brush has been covalently attached to glass and silica by reaction in a polymer melt. Subsequently, the presence of a PEO-brush was demonstrated using contact angle measurements, X-ray photoelectron spectroscopy and ellipsometry. For five bacterial (Staphylococcus epidermidis, Staphylococcus aureus, Streptococcus salivarius, Escherichia coli and Pseudomonas aeruginosa) and two yeast strains (Candida albicans and Candida tropicalis), adhesion to PEO-brushes was compared with adhesion to bare glass in a parallel plate flow chamber. The initial deposition rates of Sta. epidermidis, Sta. aureus and Str. salivarius to glass were relatively high, between 2400 and 2600 cm "2 s "1 , while E. coli and P. aeruginosa deposited much more slowly. The initial deposition rates of the yeasts to glass were 144 and 444 cm "2 s "1 for C. albicans GB 1/2 and C. tropicalis GB 9/9, respectively. Coating of the glass surface with a PEO-brush yielded more than 98 % reduction in bacterial adhesion, although for the more hydrophobic P. aeruginosa a smaller reduction was observed. For both yeast species adhesion suppression was less effective than for the bacteria and here too the more hydrophobic C. tropicalis showed less reduction than the more hydrophilic C. albicans. The PEO-brush had a thickness of 22 nm in water, as inferred from ellipsometry. Assuming that on bare glass the adhered micro-organisms are positioned only a few nanometers away from the surface and that the brush keeps them at a distance of 22 nm, it is calculated that the brush yields a sevenfold attenuation of the Lifshitz-Van der Waals attraction to the surface between the micro-organisms and the surface. Decreased Lifshitz-van der Waals attraction may be responsible for the suppression of the microbial adhesion observed.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inhibition of adhesion of yeasts and bacteria by poly(ethylene oxide)-brushes on glass in a parallel plate flow chamber

et al. 2003

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“…In order to compare our results with the previous work [36], a resolution of~0.2 nm is required and, on this scale, one cannot usefully represent ionic and molecular electric charge distributions by a continuum -the charge density is neither uniform nor static. This rendered DLVO theory inadequate [39,40]. Although monovalent ions in solution might be represented by a continuum characterized by a Debye screening length, κ À 1 , this approach cannot be used to describe divalent cations because the latter can take part in bridging between negatively-charged molecular moieties [41].…”

Section: Mathematical Models and Computer Simulation Theory And Mathementioning

confidence: 99%

Interaction of protamine with gram‐negative bacteria membranes: possible alternative mechanisms of internalization in Escherichia coli, Salmonella typhimurium and Pseudomonas aeruginosa

Pink

Hasan

Quinn

et al. 2014

Journal of Peptide Science

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This study was concerned with the interaction between the cationic antimicrobial peptide, protamine (Ptm) and the cytoplasmic membranes of the gram-negative bacteria Escherichia coli, Salmonella typhimurium and Pseudomonas aeruginosa. The objective of the study was to explain the observed paradox of internalization without permanent disruption of the cell envelope. We carried out Monte Carlo computer simulation of Ptm in an aqueous environment in the presence of~100 mM NaCl and model membranes consisting of either (65:35) or (75:25) PE:PG molar ratios. The (75:25) model, representative of the gram-negative cytoplasmic membrane, showed that the Ptm center of mass remained at least 7 nm from the membrane surface leading to the prediction that Ptm would not internalize via disruption of the inner membrane.By using immunoelectron microscopy of Ptm-treated cells, we showed that Ptm internalization to the cytoplasm took place rapidly in the presence or absence of the outer envelope. Ultrastructural examination revealed no obvious morphological changes to cells that were treated with subinhibitory or bactericidal levels of Ptm. Reconstituted phospholipid bilayers were constructed and were unperturbed by Ptm treatment over a wide range of concentrations and applied transmembrane voltages. We conclude that in the cases of the cell envelopes of E. coli, S. typhimurium and P. aeruginosa, Ptm internalized by means independent of the phospholipid bilayer, most likely mediated by one or more membrane proteins such as cation-selective barrel-like proteins. Work is currently underway to test this hypothesis.

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“…2 Consequently, a variety of different surface modifications has been developed, based on both physical and chemical techniques, to discourage microbial adhesion. The wettability of biomaterial surfaces, for instance, can be increased by plasma treatment, but this effect is most often only transient 3 and effective inhibition of microbial adhesion is absent after several months.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Surface-Grafted Polyacrylamide Brushes and Their Inhibition of Microbial Adhesion

et al. 2007

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A method is presented to prevent microbial adhesion to solid surfaces exploiting the unique properties of polymer brushes. Polyacrylamide (PAAm) brushes were grown from silicon wafers by atom transfer radical polymerization (ATRP) using a three-step reaction procedure consisting of immobilization of a coupling agent γ-aminopropyltriethoxysilane, anchoring of an ATRP initiator 4-(chloromethyl)benzoyl chloride, and controlled radical polymerization of acrylamide. The surfaces were characterized by X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, ellipsometry, and contact-angle measurements. The calculated grafting density pointed to the presence of a dense and homogeneous polymer brush. Initial deposition rates, adhesion after 4 h, and detachment of two bacterial strains (Staphylococcus aureus ATCC 12600 and Streptococcus salivarius GB 24/9) and one yeast strain (Candida albicans GB 1/2) to both PAAm-coated and untreated silicon surfaces were investigated in a parallel plate flow chamber. A high reduction (70−92%) in microbial adhesion to the surface-grafted PAAm brush was observed, as compared with untreated silicon surfaces. Application of the proposed grafting method to silicone rubbers may offer great potential to prevent biomaterials-centered infection of implants.

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The DLVO theory in microbial adhesion

Cited by 862 publications

References 78 publications

Inhibition of adhesion of yeasts and bacteria by poly(ethylene oxide)-brushes on glass in a parallel plate flow chamber

Inhibition of adhesion of yeasts and bacteria by poly(ethylene oxide)-brushes on glass in a parallel plate flow chamber

Interaction of protamine with gram‐negative bacteria membranes: possible alternative mechanisms of internalization in Escherichia coli, Salmonella typhimurium and Pseudomonas aeruginosa

Synthesis and Characterization of Surface-Grafted Polyacrylamide Brushes and Their Inhibition of Microbial Adhesion

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