The Dna Content of Spermatozoa From Different Strains of Mice

Moore, G. P. M.; Beatty, R. A.

doi:10.1530/jrf.0.0380473

Cited by 6 publications

(2 citation statements)

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“…Feulgen staining in Series 1 of human material was conducted as for the rabbit. Feulgen preparations for Series 2 of human material were made by a procedure based on those of Böhm, Sprenger, Schlüter & Sandritter (1968) and Moore & Beatty (1974). Thirty air-dried smears, prepared from a single ejaculate, were fixed for 90 min in a methanol : formalin : acetic acid mixture (85:10:5 by vol.…”

Section: Preparationsmentioning

confidence: 99%

The Recognition and Incidence of Haploid and Polyploid Spermatozoa in Man, Rabbit and Mouse

Chrothers¹,

Beatty²

1975

Reproduction

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The existence of polyploid mammalian spermatozoa has been inferred from studies of Feulgen-DNA absorption. Rabbit spermatozoa fell into two discrete groups with mean absorptions close to a 1:2 ratio (inferred to be haploids and diploids respectively) ; simple visual appraisal of the size of the head or nucleus gave an identical classification. The incidences of ploidy classes were 98\m=.\94%haploid, 1\m=.\06%diploid, 0\m=.\00%higher than diploid (N= 3010; from DNA measurements and visual appraisal of the size in a rabbit chosen to have a high incidence of diploids) and, correspondingly, 99\m=.\691%,0\m=.\308%,0\m=.\001% (N=138001; from sixty-nine unselected rabbits, scored by visual appraisal of the size of the sperm head). In man also, virtually discrete groups with absorptions close to a 1:2 ratio existed and were inferred to be haploids and diploids respectively. A few human spermatozoa were found with absorptions corresponding to a ploidy of three and/or four. Visual appraisal of the size of the human sperm nucleus as Small, Medium or Large was only a partial guide to ploidy. All Small human spermatozoa measured for DNA absorption were found to be haploid. About two-thirds of Medium human spermatozoa were found, however, to be haploid, and some Large spermatozoa were haploid or diploid. The incidences of ploidy classes in the human were 99\m=.\37%haploid, 0\m=.\56%diploid, 0\m=.\07%higher than diploid (N=5554; with consistency between duplicate slides and between two subjects; from DNA measurements and visual appraisal of nuclear size). The estimated incidence of diploid human spermatozoa is consistent with the known incidence of triploid fetuses. In a mouse with a putatively high incidence of diploids, all 1000 DNA measurements were nevertheless within the haploid range, with one diploid encountered outside the main sampling.

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Section: Preparationsmentioning

confidence: 99%

The Recognition and Incidence of Haploid and Polyploid Spermatozoa in Man, Rabbit and Mouse

Chrothers¹,

Beatty²

1975

Reproduction

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“…In comparison with domestic fowl, where Mirsky and Ris [1949] and Vendrely et al [1956] determined DNA content per sperm as 1.26 pg and 1.1 pg, respectively, that of the turkey is distinctly higher. Moore and Beatty [1974] found that the variation of sperm DNA content in males within strains was not significant, but strain means differed significantly from each other. Similarly, we found significant differences of DNA content among maternal and paternal lines.…”

Section: Discussionmentioning

confidence: 84%

DNA content of spermatozoa in different genetic lines of turkeys

Lamošová

Baumgartner

1988

Gamete Research

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The variability of DNA content in turkey spermatozoa of four different lines and its correlation with body weight and sperm concentration were studied. In lines selected for lower body weight the DNA content was 2.034 and 2.036 pg per spermatozoon. In lines selected for higher body weight the DNA content was 2.267 and 2.370 pg per spermatozoon. Sperm concentration in 1 mm3 of semen, however, was higher in lines with a lower body weight (6.08-6.21 million) in comparison with lines selected for higher body weight (5.46-5.67 million). The correlations between the DNA content and sperm concentration were negative (r ranged from -.457 to -.860).

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