The DNA puff gene BhC4-1 of Bradysia hygida is specifically transcribed in early prepupal salivary glands of Drosophila melanogaster

Monesi, Nádia; Jacobs-­Lorena, Marcelo; Paçó-Larson, Maria Luísa

doi:10.1007/s004120050342

Cited by 43 publications

(57 citation statements)

References 38 publications

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“…DNA was extracted following phenol/chloroform methodology (Monesi et al, 1998) and quantified on 0.8% agarose gels. For all samples, DNA concentration was diluted to 10 ng/µl for further analysis.…”

Section: Pool C (M3+m4+m5+m6)mentioning

confidence: 99%

Individual contributions to pooled-milt fertilizations of silver catfish Rhamdia quelen

Ribolli

Zaniboni‐Filho

2009

Neotrop. ichthyol.

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Supplementary stocking of fish in natural environments is a way to mitigate or compensate for the changes imposed on wild populations by river damming. Since little is known about the genetic composition of the supplementary stocks obtained by pooled-milt fertilization, the aim of this study was to determine the individual contributions of male jundiá Rhamdia quelen to offspring. Sperm from four males were mixed using equal volume of sperm from each of the males to fertilize eggs from only one female, kept in three blend with six males and three females. The proportions of larvae sired by the different males were quantified using five polymorphic DNA microsatellite loci. Analysis of these loci allowed paternal determination of 84% of the progeny, at a 0.972 combined exclusion probability. Broodstock milt had good fertilizing capacity when used alone, but when pooled the fertilizing capacities, its fertilizing possibility varied from 4 to 65%. Results show that milt pools favor gametes of some males over others, thus reducing the progeny's genetic variability.A estocagem de suplementação de peixes em ambientes naturais é uma das maneiras de mitigar ou compensar as alterações impostas pelo represamento dos rios às populações selvagens. Pouco se conhece sobre a composição genética dos estoques repovoadores obtidos através do manejo reprodutivo com fertilizações com o uso de pool de sêmen, de maneira que este trabalho teve como objetivo verificar a contribuição individual de machos de jundiá (Rhamdia quelen) na progênie. O mesmo volume de sêmen de quatro machos foi misturado para fertilizar os ovócitos de uma única fêmea, sendo mantidos em três combinações com seis machos e três fêmeas. A proporção de larvas geradas de diferentes machos foi quantificada através de cinco marcadores microssatélites. A análise dos cinco locus permitiu a determinação da paternidade de 84% da progênie, com 0,972 de probabilidade de exclusão combinada. O sêmen dos reprodutores apresentou boa capacidade de fertilização quando utilizados separadamente, porém, quando utilizados em pool apresentaram capacidade de fertilização entre 4 a 65%. Os resultados revelam que o pool de sêmen favorece alguns machos sobre outros, reduzindo a variabilidade genética da progênie.

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Section: Pool C (M3+m4+m5+m6)mentioning

confidence: 99%

Individual contributions to pooled-milt fertilizations of silver catfish Rhamdia quelen

Ribolli

Zaniboni‐Filho

2009

Neotrop. ichthyol.

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“…Extraction and quantification of the DNA genome DNA extraction was carried out using the phenol/chloroform method (Monesi et al, 1998). Samples of muscle tissue, of approximately 100 mg, were ground in liquid nitrogen.…”

Section: Molecular Variability In Brycon Cf Pesumentioning

confidence: 99%

Molecular variability in Brycon cf. pesu Muller and Troschel, 1845 (Characiformes: Characidae) from the Araguaia-Tocantins Basin

Panarari-Antunes

Prioli

Prioli³

et al. 2008

Genet. Mol. Res.

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AbSTRACT. Brycon pesu is a small-sized fish distributed throughout the Amazon and Orinoco Basins and other coastal basins of northeastern South America. Brycon cf. pesu specimens from the Araguaia-Tocantins Basin are currently separated into two morphotypes, Brycon sp1 and Brycon sp2, owing to different coloration of their anal fin. Brycon sp2 has a reddish margin stripe on the anal fin which morphologically distinguishes it from Brycon sp1. In the present research, nuclear and mitochondrial markers were used to test the hypothesis that the Brycon sp1 and Brycon sp2 morphotypes are distinct species. Specimens from the two morphotypes were collected from the Lajeado Hydroelectric Plant and the Palmas River in the AraguaiaTocantins Basin. Thirty-five loci obtained by the amplification of five inter-simple sequence repeat primers were analyzed but no species-specific bands were detected. Electrophoretic profiles obtained from 5S rDNA non-transcribed spacer amplification failed to show any differentiation in morphotypes. These results were corroborated by nucleotide sequence analysis of the mtDNA control region, in which 24 polymorphic nucleotide sites, representing a polymorphism rate of only 5%, were detected. The low rates of polymorphism detected by inter-simple sequence repeat, non-transcribed spacer and mtDNA D-loop markers strongly reject the hypothesis that the two morphotypes Brycon sp1 and Brycon sp2 represent distinct species within Brycon cf. pesu. Further studies are needed to obtain conclusive data on the notion that the coloration of the anal fin is an intraspecific polymorphism, possibly related to environmental factors.

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“…The availability of a transformation system and mutant collections, coupled to genomic information in the model system Drosophila melanogaster, prompted us to perform functional assays of DNA puff genes in a transgenic context. In transgenesis, BhC4-1 expression in the salivary glands is a late response to the increase in ecdysone titers at the onset of metamorphosis (Basso et al, 2002;Monesi et al, 1998). Genetic interaction experiments have demonstrated that the products of the ecdysoneinduced early genes, BR-C, E74, and E75 participate to differing extents in the induction of BhC4-1-lacZ in the salivary gland (Basso et al, 2006).…”

mentioning

confidence: 99%

Functional and bioinformatics analyses reveal conservation of cis‐regulatory elements between sciaridae and drosophilidae

Lecci

Malta

Flausino

et al. 2008

Genesis

Self Cite

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Summary: The sciarid DNA puff C4 BhC4-1 gene is amplified and transcribed in salivary glands at the end of the larval stage. In transgenic Drosophila, the BhC4-1 promoter drives transcription in prepupal salivary glands and in the ring gland of late embryos. A bioinformatics analysis has identified 162 sequences similar to distinct regions of the BhC4-1 proximal promoter, which are predominantly located either in 5 0 or 3 0 regions or introns in the Drosophila melanogaster genome. A significant number of the identified sequences are found in the regulatory regions of Drosophila genes that are expressed in the salivary gland. Functional assays in Drosophila reveal that the BhC4-1 proximal promoter contains both a 129 bp (2186/258) salivary gland enhancer and a 67 bp

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The DNA puff gene BhC4-1 of Bradysia hygida is specifically transcribed in early prepupal salivary glands of Drosophila melanogaster

Cited by 43 publications

References 38 publications

Individual contributions to pooled-milt fertilizations of silver catfish Rhamdia quelen

Individual contributions to pooled-milt fertilizations of silver catfish Rhamdia quelen

Molecular variability in Brycon cf. pesu Muller and Troschel, 1845 (Characiformes: Characidae) from the Araguaia-Tocantins Basin

Functional and bioinformatics analyses reveal conservation of cis‐regulatory elements between sciaridae and drosophilidae

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