The dnaJ gene as a novel phylogenetic marker for identification of Vibrio species

An Improved Detection and Quantification Method for the Coral Pathogen Vibrio coralliilyticus

“…Due to the relative homogeneity of the 16S rRNA gene in Vibrio species, alternative gene targets for identification have been investigated and indeed the dnaJ gene has been suggested as a promising candidate [34], [68]. Pollock et al (2010) previously designed a real-time PCR assay with TaqMan fluorescent probes for the dnaJ gene to detect V. coralliilyticus in environmental samples which was successful in specifically detecting twelve of thirteen tested V. coralliilyticus isolates.…”

Oyster mortality

“…francensis are translucent and measure around 1mm after 48 hours (Tison and Seidler, 1983;Garnier et al, 2008). Two molecular tools have been developed, based on amplification using real-time PCR of a DNAj gene (GenBank # AB263018) coding for a heat shock protein (Nhung et al, 2007). The first is a multiplex real-time PCR permitting the detection of both V. aestuarianus and V. splendidus species from isolated bacteria of culture agar medium (http://www.eurl-mollusc.eu/SOPs).…”

Multiplex PCR for the detection and differentiation of Vibrio parahaemolyticus strains using the groEL, tdh and trh genes

“…Among the medically important Vibrio species, Vibrio parahaemolyticus is recognised as the leading cause of human gastroenteritis associated with seafood consumption [1,2], and is an important seafood-borne pathogen globally [3]. It is a Gramnegative, facultative halophilic bacterium common in aquatic environments worldwide, including estuaries and seawater, and is frequently isolated from zooplankton, coastal fish, and shellfish [4].…”