The dog 2.0: Lessons learned from the past

Broeckx, Bart J. G.

doi:10.1016/j.theriogenology.2020.01.043

Cited by 17 publications

(15 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this context, according to Broeckx [ 55 ], once reproductive aims covering the specific framework of dog breeds have been defined and the problems for that particular populations have been identified, the approach to pursue and reach possible solutions is similar, as it always stems from the identification of un(desirable) phenotypes and the genotype behind them.…”

Section: Discussionmentioning

confidence: 99%

Does Functionality Condition the Population Structure and Genetic Diversity of Endangered Dog Breeds under Island Territorial Isolation?

Puente

Barro

Giraldo

et al. 2020

Animals

View full text Add to dashboard Cite

Despite the undefinition of the origins of Ca de Rater (CR) and Ca de Bestiar (CB) dogs, references to these endangered autochthonous breeds highlighted their ratting/pet and shepherding/guard skills for centuries. Genealogical historical records were traced back to founders. Founder number in the reference population (146 and 53 for CR and CB, respectively), historical and reference maximum generations traced (eight and seven for CR and CB, respectively), and historical average number of complete generations (1.04 for both breeds) were determined. Structure assessment revealed the existence of subpopulations regarding criteria such as breeders (75 and 17), breeder location (32 and eight), owners (368 and 198), and owner location (73 and 51) for CR and CB, respectively. Average inbreeding (F) within breed subpopulations ranged from 0.27–1.20% for CB breeders and the rest of subpopulation criteria for both breeds, respectively, except for CB owners and owner location. F ranged from 0.27–1.41% for CB historical population and CR current population, respectively. The study of genetic diversity revealed a relatively similar genetic background between subpopulations. Average coancestry between and within breeds suggested a similar evolutionary process. However, Mann–Whitney U test determined significant differences for diversity parameters (F, ΔR, coancestry, nonrandom mating degree, maximum, complete, and equivalent generations, ΔF, and genetic conservation index) between breeds and their functionalities. Conclusively, functionality in dog breeds may determine the genetic diversity evolution of endangered breeds, even when these share the same geographic isolation conditions.

show abstract

Section: Discussionmentioning

confidence: 99%

Does Functionality Condition the Population Structure and Genetic Diversity of Endangered Dog Breeds under Island Territorial Isolation?

Puente

Barro

Giraldo

et al. 2020

Animals

View full text Add to dashboard Cite

show abstract

“…The negative consequences of current breeding strategies are becoming increasingly apparent in terms of population health status, and the welfare of individuals is starting to be discussed [6]. In the previous ten years, the genetic variability of many breeds has been analyzed across countries.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Genetic Diversity in the Czech Spotted Dog

Machová

Kranjčevičová

Vostrý

et al. 2020

Animals

View full text Add to dashboard Cite

Loss off genetic diversity negatively affects most of the modern dog breeds. However, no breed created strictly for laboratory purposes has been analyzed so far. In this paper, we sought to explore by pedigree analysis exactly such a breed—the Czech Spotted Dog (CSD). The pedigree contained a total of 2010 individuals registered since the second half of the 20th century. Parameters such as the mean average relatedness, coefficient of inbreeding, effective population size, effective number of founders, ancestors and founder genomes and loss of genetic diversity—which was calculated based on the reference population and pedigree completeness—were used to assess genetic variability. Compared to the founding population, the reference population lost 38.2% of its genetic diversity, of which 26% is due to random genetic drift and 12.2% is due to the uneven contribution of the founders. The reference population is highly inbred and related. The average inbreeding coefficient is 36.45%, and the mean average relatedness is 74.83%. The effective population size calculated based on the increase of inbreeding coefficient is 10.28. Thus, the Czech Spotted Dog suffered significant losses of genetic diversity that threaten its future existence.

show abstract

“…Unlike genome wide association studies, a small number, or even a single case may be sufficient to identify underlying variant as in this study. If successful, WGS can help identify risk variants early on in the disease emergence process, hence eliminating it before the disease becomes widespread within the population, which is especially important in dogs due to high levels of inbreeding, popular sire effects and relatively fast turnover of progeny [ 45 , 46 ]. A limitation to using WGS as with other sequencing techniques, is the inherent difficulty in the detection of structural variants, for example, transposons, inversions, or large insertions and deletions which our current WGS pipeline cannot identify.…”

Section: Discussionmentioning

confidence: 99%

Identification of a variant in NDP associated with X-linked retinal dysplasia in the English cocker spaniel dog

et al. 2021

View full text Add to dashboard Cite

Purpose Three related male English Cocker Spaniels (ECS) were reported to be congenitally blind. Examination of one of these revealed complete retinal detachment. A presumptive diagnosis of retinal dysplasia (RD) was provided and pedigree analysis was suggestive of an X-linked mode of inheritance. We sought to investigate the genetic basis of RD in this family of ECS. Methods Following whole genome sequencing (WGS) of the one remaining male RD-affected ECS, two distinct investigative approaches were employed: a candidate gene approach and a whole genome approach. In the candidate gene approach, COL9A2, COL9A3, NHEJ1, RS1 and NDP genes were investigated based on their known associations with RD and retinal detachment in dogs and humans. In the whole genome approach, affected WGS was compared with 814 unaffected canids to identify candidate variants, which were filtered based on appropriate segregation and predicted pathogenic effects followed by subsequent investigation of gene function. Candidate variants were tested for appropriate segregation in the ECS family and association with disease was assessed using samples from a total of 180 ECS. Results The same variant in NDP (c.653_654insC, p.Met114Hisfs*16) that was predicted to result in 15 aberrant amino acids before a premature stop in norrin protein, was identified independently by both approaches and was shown to segregate appropriately within the ECS family. Association of this variant with X-linked RD was significant (P = 0.0056). Conclusions For the first time, we report a variant associated with canine X-linked RD. NDP variants are already known to cause X-linked RD, along with other abnormalities, in human Norrie disease. Thus, the dog may serve as a useful large animal model for research.

show abstract

The dog 2.0: Lessons learned from the past

Cited by 17 publications

References 54 publications

Does Functionality Condition the Population Structure and Genetic Diversity of Endangered Dog Breeds under Island Territorial Isolation?

Does Functionality Condition the Population Structure and Genetic Diversity of Endangered Dog Breeds under Island Territorial Isolation?

Analysis of Genetic Diversity in the Czech Spotted Dog

Identification of a variant in NDP associated with X-linked retinal dysplasia in the English cocker spaniel dog

Contact Info

Product

Resources

About