The dopamine response in mouse neuroblastoma cells is mediated by serotonin 5HT3 receptors

Neijt, H.C.; Vijverberg, Henk P.M.; Bercken, Joep van den

doi:10.1016/0014-2999(86)90374-2

Cited by 56 publications

(31 citation statements)

References 9 publications

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“…No attempt was made to characterize in detail the 5-HT receptor mediating the inward current response to 5-HT in NIE-115 cells, since there is already substantial evidence that this effect is due to the activation of the 5-HT3 receptor subtype (Neijt et al, 1986;. The blockade by GR 38032F of 5-HT-induced currents described here adds to this evidence.…”

Section: Discussionmentioning

confidence: 86%

“…Consistent with these reports, it has been proposed that a simultaneous increase in membrane conductance to Na+ and K+ mediates both the 5-HT-evoked depolarization and dopamine-induced inward current in NIE-115 cells (Peters & Usherwood, 1983;Kato & Narahashi, 1982). The latter response has recently been attributed to 5-HT3 rather than dopamine receptor activation (Peters & Usherwood, 1984;Neijt et al, 1986).…”

Section: Discussionmentioning

confidence: 99%

“…Similar depolarizing responses to 5-HT have been observed in several neuronal clonal cell lines which are amenable to advanced electrophysiological techniques. In murine NIE-115 neuroblastoma cells, 5-HT increases membrane conductance to monovalent cations, generating a depolarizing response under current-clamp conditions (Guharay & Usherwood, 1981;Peters & Usherwood, 1983), and an inward current when the membrane is voltage-clamped at negative holding potentials ( (Neijt et al, 1986). Both the 5-HT-induced depolarization and inward current are antagonized by the selective 5-HT3 receptor antagonists MDL 72222 and ICS 205-930, but are unaffected by antagonists acting at '5-HT1-like' or 5-HT2 sites .…”

Section: Introductionmentioning

confidence: 99%

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The properties of 5‐HT₃ receptors in clonal cell lines studied by patch‐clamp techniques

Lambert

Peters

Hales

et al. 1989

British J Pharmacology

131

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1 The characteristics of transmembrane currents evoked by 5-hydroxytryptamine (5-HT) in the neuroblastoma x Chinese hamster brain cell line On outside-out membrane patches excised from both NCB-20 and NlE-115 cells, 5-HT induced small inward currents which could not be clearly resolved into discrete single channel events. Such responses were: (i) reversibly antagonized by GR 38032F (1 nM) (ii) reversed in sign at 0 mV, and (iii) subject to desensitization. 6 Fluctuation analysis of inward currents evoked by 5-HT (1 M) in NlE-1 15 cells suggests that 5-HT gates a channel with a conductance of approximately 310fS. Such a relatively small conductance could readily explain why the response of outside-out membrane patches to 5-HT cannot at present be resolved into clear single channel events.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The properties of 5‐HT₃ receptors in clonal cell lines studied by patch‐clamp techniques

Lambert

Peters

Hales

et al. 1989

British J Pharmacology

131

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“…In mouse NIE-115 neuroblastoma cells, 5-HT3 receptors have been investigated in detail. From these cells the functional characteristics of the 5-HT3 receptor-mediated macroscopic whole-cell ion current (Neijt et al, 1986;Lambert et al, 1989) and microscopic single channel events Hooft & Vijverberg, 1995), as well as the ligand-binding profile of the recognition site (Hoyer & Neijt, 1988;Lummis et al, 1990) and the primary sequence of the cloned 5-HT3 receptor subunit (5-HT3R-A) and a splice variant (5-HT3R-A,) have been reported (Hope et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Selection of distinct conformational states of the 5‐HT₃ receptor by full and partial agonists

Hooft

Vijverberg

1996

British J Pharmacology

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2 The concentration-dependent activation and desensitization of the ion currents evoked by the agonists yield the potency order: mCPBG > 5-HTQ % 5-HT > > tryptamine > dopamine, and the efficacy order: 5-HT mCPBG 5-HTQ> > dopamine tryptamine. Thus, 5-HT, 5-HTQ and mCPBG are full agonists, whereas dopamine and tryptamine are partial agonists at the 5-HT3 receptor. 3 Full and partial agonists cause complete cross-desensitization and activate single channels with similar conductances and open lifetimes. This shows that full and partial agonists act on the same population of 5-HT3 receptors. 4 The time course of recovery from desensitization depends on the agonist used. Recovery from partial agonist-induced desensitization is single exponential, whereas the desensitization induced by full agonists recovers with sigmoid kinetics, suggesting at least 3 steps between 4 states. 5 During the process of recovery from cross-desensitization, the full agonists activate a larger fraction of the 5-HT3 receptors than the partial agonists, irrespective of the agonist used to induce desensitization. 6 It is concluded that full and partial agonists induce distinct desensitized states and, during recovery from desensitization, recognize distinct conformations of unoccupied 5-HT3 receptors. This conformational selection is likely to account for the different efficacies of full and partial 5-HT3, receptor agonists.

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“…The presence of NSE mRNA and the absence of GFAP, NF and PLP mRNAs in IMR32 cells irrespective of differentiation suggests that these cells are committed to a neural cell pathway. Neuronally-differentiated neuroblastoma cells have been shown to contain neurotransmitter receptors [12,24] and enzymes [12,13,41]. They produce a number of neurotransmitters such as dopamine, noradrenaline and serotonin [13], and contain noradrenergic uptake systems [30].…”

Section: Discussionmentioning

confidence: 99%

Neural differentiation of the human neuroblastoma cell line IMR32 induces production of a thyrotropin-releasing hormone-like peptide

Rondeel¹,

Klootwijk

Linkels

et al. 1994

Brain Research

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The human neuroblastoma cell line IMR32 produces and secretes substantial amounts of TRH-immunoreactivity (TRH-IR) as measured with radioimmunoassay (RIA) using the nonspecific antiserum 4319. It was found that synthesis of TRH-IR is dependent on neural differentiation: under serum-free conditions these cells exhibit neural characteristics as defined by morphological and biochemical standards. After culture for 2-5 days in serum-free medium cells grew large neural processes and expressed neuron-specific markers whereas glial-specific markers were absent. TRH-IR became detectable after 4-8 days serum-free conditions. Northern blot and chromatographic analysis, however, failed to detect proTRH mRNA and authentic TRH in these cells. Moreover, TRH-IR was undetectable in the RIA using TRH-specific antiserum 8880. TRH-IR produced by differentiated cells was retained on a QAE Sephadex A-25 anion-exchange column and thus negatively charged. HPLC analysis showed coelution with the synthetic peptide pGlu-Glu-ProNH e. Study of the mechanisms regulating production of this novel peptide in these cells should further elucidate the role differentiation plays in the synthesis of neuropeptides.

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The dopamine response in mouse neuroblastoma cells is mediated by serotonin 5HT3 receptors

Cited by 56 publications

References 9 publications

The properties of 5‐HT₃ receptors in clonal cell lines studied by patch‐clamp techniques

The properties of 5‐HT₃ receptors in clonal cell lines studied by patch‐clamp techniques

Selection of distinct conformational states of the 5‐HT₃ receptor by full and partial agonists

Neural differentiation of the human neuroblastoma cell line IMR32 induces production of a thyrotropin-releasing hormone-like peptide

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The dopamine response in mouse neuroblastoma cells is mediated by serotonin 5HT3 receptors

Cited by 56 publications

References 9 publications

The properties of 5‐HT3 receptors in clonal cell lines studied by patch‐clamp techniques

The properties of 5‐HT3 receptors in clonal cell lines studied by patch‐clamp techniques

Selection of distinct conformational states of the 5‐HT3 receptor by full and partial agonists

Neural differentiation of the human neuroblastoma cell line IMR32 induces production of a thyrotropin-releasing hormone-like peptide

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The properties of 5‐HT₃ receptors in clonal cell lines studied by patch‐clamp techniques

The properties of 5‐HT₃ receptors in clonal cell lines studied by patch‐clamp techniques

Selection of distinct conformational states of the 5‐HT₃ receptor by full and partial agonists