The Dose–Response Relationships for EROD and GST Induced by Polyaromatic Hydrocarbons in Carassius auratus

Lu, Guanghua; Wang, C.; Zhu, Zhen

doi:10.1007/s00128-008-9622-3

Cited by 67 publications

(32 citation statements)

References 22 publications

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“…In a recent study, a hepatic GST activity has been induced in eel, Anguilla anguilla, after 2 and 46 h waterborne naphthalene exposure (Teles et al 2003). A more recent study carried out by Lu et al (2009) demonstrated that GST induction potential of five PAHs in gold fish, Carassius auratus, increased in the order fluoranthene \ fluorine \ benzo(h)fluoranthene \ benzo(g,h,i) perylene \ indeno(1,2,3-cd)-pyrene. In the present study, only the lowest dose (1 mg/kg bw) of phenanthrene, fluoranthene and chrysene resulted in induction (up to 1.5-fold) of hepatic total GST activities (measured with the CDNB as the substrate) in Nile tilapia where as pyrene had no significant effect on this enzyme.…”

Section: Discussionmentioning

confidence: 95%

“…Hence these organic compounds are called as AhR agonists (Whyte et al 2000;Timme-Laragy et al 2007). In vitro studies with mammalian and piscine cell cultures and a few in vivo studies with some fish species have compared the EROD induction potency of different PAHs (Till et al 1999;Fent and Batscher 2000;Whyte et al 2000;Basu et al 2001;Behrens et al 2001;Willett et al 2001;Bosveld et al 2002;Billard et al 2004;Lu et al 2009). In general PAHs with five-or more rings are good CYP1A inducers but inconsistent information is available on EROD induction potential of PAHs containing two to four fused rings.…”

Section: Discussionmentioning

confidence: 99%

“…Induction of CYP1A activity has been used to infer effects such as cancer related liver lesions and reproductive impairments in fish (Whyte et al 2000). Numerous in vitro studies with mammalian and piscine cell cultures and a few in vivo studies with some fish species indicate that inducibility of EROD varies with the type of PAH (Till et al 1999;Whyte et al 2000;Basu et al 2001;Behrens et al 2001;Willett et al 2001;Bosveld et al 2002;Billard et al 2004;Lu et al 2009). Most of these studies have focused on high molecular weight PAHs with five-or more fused rings.…”

Section: Introductionmentioning

confidence: 98%

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Modulation of ethoxyresorufin O-deethylase and glutathione S-transferase activities in Nile tilapia (Oreochromis niloticus) by polycyclic aromatic hydrocarbons containing two to four rings: implications in biomonitoring aquatic pollution

Pathiratne

Hemachandra

2010

Ecotoxicology

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Despite ubiquity of polycyclic aromatic hydrocarbons (PAHs) in the tropical environments, little information is available concerning responses of tropical fish to PAHs and associated toxicity. In the present study, effects of five PAHs containing two to four aromatic rings on hepatic CYP1A dependent ethoxyresorufin O-deethylase (EROD), glutathione S-transferase (GST) and serum sorbitol dehydrogenase (SDH) activities in Nile tilapia, a potential fish species for biomonitoring pollution in tropical waters, were evaluated. Results showed that EROD activities were induced by the PAHs containing four aromatic rings (pyrene and chrysene) in a dose dependent manner. However PAHs with two to three aromatic rings (naphthalene, phenanthrene and fluoranthene) caused no effect or inhibition of EROD activities depending on the dose and the duration. Fluoranthene was the most potent inhibitor. SDH results demonstrated that high doses of fluoranthene induced hepatic damage. GST activity was induced by the lowest dose of phenanthrene, fluoranthene and chrysene but high doses had no effect. The results indicate that induction of EROD enzyme in Nile tilapia is a useful biomarker of exposure to PAHs such as pyrene and chrysene. However EROD inhibiting PAHs such as fluoranthene in the natural environment may modulate the EROD inducing potential of other PAHs thereby influencing PAH exposure assessments.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Modulation of ethoxyresorufin O-deethylase and glutathione S-transferase activities in Nile tilapia (Oreochromis niloticus) by polycyclic aromatic hydrocarbons containing two to four rings: implications in biomonitoring aquatic pollution

Pathiratne

Hemachandra

2010

Ecotoxicology

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“…Measurements of xenobiotic concentrations are expensive and do not supply the amount of the impact on the biological compartment (Solé et al, 2009;Schirmer et al, 2011). The recent advances in the ecotoxicology field had supplied a number of the biomarkers in organism, which can be used to estimate either exposure to chemicals or resultant effects (Handy et al, 2003;Lu et al, 2009;Ramsdorf et al, 2012). Biomarkers and bioindicators has been extensively used as proxies to determine responses at individual level to stressors, with biomarkers being more species-specific and with higher variability of responses compared to bioindicators (Schulz and MartinsJunior, 2001;Solé et al, 2010;Linde-Arias et al, 2008a, b;Barrilli et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Biomarkers and bioindicators of the environmental condition using a fish species (Pimelodus maculatus Lacepède, 1803) in a tropical reservoir in Southeastern Brazil

et al. 2017

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The Funil Reservoir receives a large amount of xenobiotics from the Paraíba do Sul River (PSR) from large number of industries and municipalities in the watershed. This study aimed to assess environmental quality along the longitudinal profile of the Paraíba do Sul River-Funil Reservoir system, by using biomarkers and bioindicators in a selected fish species. The raised hypothesis is that Funil Reservoir acts as a filter for the xenobiotics of the PSR waters, improving river water quality downstream the dam. Two biomarkers, the ethoxyresorufin-O-deethylase activity (EROD), measured as fluorimetricly in S9 hepatic fraction, and the micronuclei frequency (MN), observed in erythrocytes of the cytoplasm, and three bioindicators, the hepatosomatic index (HSI), gonadosomatic index (GSI) and condition factor (CF) were used in Pimelodus maculatus, a fish species widely distributed in the system. Four zones were searched through a longitudinal gradient: 1, river upstream from the reservoir; 2, upper reservoir; 3, lower reservoir; 4, river downstream of the reservoir. EROD activity and HSI and GSI had significant differences among the zones (P<0.05). The upper reservoir had the lowest EROD activity and HSI, whereas the river downstream of the reservoir had the highest EROD and lowest GSI. The river upstream from the reservoir showed the highest HSI and GSI. It is suggested that the lowest environmental condition occur at the river downstream of the reservoir, where it seems to occur more influence of xenobiotics, which could be associated with hydroelectric plant operation. The hypothesis that Funil reservoir acts as a filter decanting pollution from the Paraíba do Sul River waters was rejected. These results are novel information on this subject for a native fish species and could be useful for future comparisons with other environments.Keywords: biomonitoring, EROD, fishes, pollutants, reservoirs. Biomarcadores e bioindicadores da condição ambiental usando uma espécie de peixe (Pimelodus maculatus

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“…Several studies have evaluated the effects of ABC transporters in fish exposed to environmental pollutants (Christine Paetzold et al, 2009;Diaz de Cerio et al, 2012). Phase I (e.g., Cyp450) and II (e.g., Gst) detoxification enzymes catalyze the reactions of xenobiotic metabolism, and the induction of phase I and II enzymes by environmental pollutants (e.g., PAHs) has been widely studied (Kopecka-Pilarczyk and Correia, 2009;Lu et al, 2009;Yeager et al, 2009). Previous studies indicated that ABC transporters could be regulated in a coordinated manner with phase I and II enzymes (Bard, 2000;Leslie et al, 2005;Xu et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Basal and benzo[a]pyrene-induced expression profile of phase I and II enzymes and ABC transporter mRNA in the early life stage of Chinese rare minnows (Gobiocypris rarus)

Yuan

Zha

et al. 2014

Ecotoxicology and Environmental Safety

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a b s t r a c t ATP-binding cassette (ABC) transporters together with phase I and II detoxification enzymes have been considered as included in a cellular detoxification system. Previous studies have highlighted the involvement of aryl hydrocarbon receptor (AHR) and Cyp1a in PAH-induced embryo toxicity. However, the response of other xenobiotic enzymes/transporters in PAH-mediated embryo toxicity is not fully characterized. In the present study, rare minnow embryos were exposed to 10 and 100 mg/L BaP within 4 h post-fertilization (hpf) up to 168 hpf. RNA was extracted at 24, 48, 96, and 168 hpf. The basal and BaP-induced expression of phase I enzyme genes (cyp1a, 1b1, and 1c1), phase II enzyme gene (gstm and ugt1a), and ABC transporter genes (abcb1, abcc1, abcc2, and abcg2) mRNA was determined using realtime PCR. Severe developmental defects (e.g., spinal deformities, pericardial and yolk-sac edema) were observed in the BaP treated groups. The basal expression showed that gstm was most strongly expressed, followed by abcb1, ugt1a, and abcc2, whereas cyp1a, 1b1, 1c1, and abcg2 showed weak expression. BaP significantly induced the mRNA expression of three CYP1s (cyp1a, 1b1, and 1c1) (p o0.05) and the ABC transporters (abcc1, abcc2, and abcg2) in a dose-dependent manner. However, the mRNA expression of Phase II enzymes (gstm, ugt1a) for the BaP treatments showed no significant difference with that of the controls. Furthermore, distinct induced patterns of these genes were observed during different exposure periods. Simultaneous up-regulation of the cyp and ABC transporter gene transcripts suggests that a possible involvement and cooperation in the detoxification process could provide protection against the BaP toxicity of rare minnows at the early life stage.

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The Dose–Response Relationships for EROD and GST Induced by Polyaromatic Hydrocarbons in Carassius auratus

Cited by 67 publications

References 22 publications

Modulation of ethoxyresorufin O-deethylase and glutathione S-transferase activities in Nile tilapia (Oreochromis niloticus) by polycyclic aromatic hydrocarbons containing two to four rings: implications in biomonitoring aquatic pollution

Modulation of ethoxyresorufin O-deethylase and glutathione S-transferase activities in Nile tilapia (Oreochromis niloticus) by polycyclic aromatic hydrocarbons containing two to four rings: implications in biomonitoring aquatic pollution

Biomarkers and bioindicators of the environmental condition using a fish species (Pimelodus maculatus Lacepède, 1803) in a tropical reservoir in Southeastern Brazil

Basal and benzo[a]pyrene-induced expression profile of phase I and II enzymes and ABC transporter mRNA in the early life stage of Chinese rare minnows (Gobiocypris rarus)

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