The Drosophila circadian clock circuit is a nonhierarchical network of peptidergic oscillators

Crespo-Flores, Sergio L.; Barber, Annika F.

doi:10.1016/j.cois.2022.100944

Cited by 5 publications

(3 citation statements)

References 64 publications

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“…At CT8, the affected pathways were primarily related to the digestive system and endocrine system, accounting for approximately 17.2% and 15.05% of the DEGs respectively (Figure S4C). Barber, 2022;Liang et al, 2017;Selcho et al, 2017) revealed that the relative expression levels (fpkm) of neuropeptide genes Neuropeptide F2 (NPF2) and short neuropeptide F (sNPF) in the mutant was significantly lower at CT24 compared to the WT (Figure 6a,b). Similarly, the relative expression levels of Neuropeptide receptor A1 (Npr A1) and Corazonin (Crz) genes were also significantly reduced at CT24 (Figure 6c,d).…”

Section: Hatching Behavior and Physiological Functions Of Cry1 Defici...mentioning

confidence: 99%

“…The physiology and behavior of most organisms are influenced by circadian rhythms, which are the internal timekeeper—biological clock—formed by organisms adapting to the 24‐h cycle of the Earth's environment (Brady et al, 2021; Crespo‐Flores & Barber, 2022; Mark et al, 2021; Ulgherait et al, 2021). The molecular model of the circadian clock was first proposed in the fruit fly ( Drosophila melanogaster ) and has been extensively studied (Ahmad et al, 2021; Bargiello & Young, 1984; Konopka & Benzer, 1971).…”

Section: Introductionmentioning

confidence: 99%

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The clock gene Cryptochrome 1 is involved in the photoresponse of embryonic hatching behavior in Bombyx mori

Yuan

Luo

Zhao

et al. 2023

Arch Insect Biochem Physiol

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The hatching of insect eggs is a classic circadian behavior rhythm controlled by the biological clock. Its function is considered to impose a daily rhythm on the embryo, allowing it to hatch within a permissible time window. However, the molecular pathways through which the clock affects embryonic hatching behavior remain unclear. Here, we utilized a clock gene Cryptochrome1 (Cry1) knockout mutant to dissect the pathways by which the circadian clock affects embryonic hatching rhythm in the silkworm. In the Cry1 mutant, the embryo hatching rhythm was disrupted. Under the constant light or constant dark incubation conditions, mutant embryos lost their hatching rhythm, while wild‐type embryos hatch exhibiting free‐running rhythm. In the light‐dark cycle (LD), the hatching rhythm of CRY1‐deficient silkworms could not be entrained by the LD photoperiod during the incubation period. The messenger RNA levels and enzymatic activities of Cht and Hel in the mutant embryos were significantly reduced at circadian time 24 (CT24). Transcriptome analysis revealed significant differences in gene expression at CT24 between the Cry1 knockout mutant and the wild‐type, with 2616 differentially expressed genes identified. The enriched Gene Ontology pathway includes enzyme activity, energy availability, and protein translation. Short neuropeptide F signaling was reduced in the CT24 embryonic brain of the mutant, the expression of the neuropeptide PTTH was also reduced and the rhythm was lost, which further affects ecdysteroid signaling. Our results suggested that the silkworm circadian clock affects neuropeptide‐hormone signaling as well as physiological functions related to hatching, which may regulate the hatching rhythm.

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Section: Hatching Behavior and Physiological Functions Of Cry1 Defici...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The clock gene Cryptochrome 1 is involved in the photoresponse of embryonic hatching behavior in Bombyx mori

Yuan

Luo

Zhao

et al. 2023

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

show abstract

“…2 a) (Kaneko and Hall 2000 ). They are divided into the following clusters: small lateral neurons (s-LNvs), large lateral neurons (l-LNvs), 5 th lateral neuron (5 th LN, also known as 5 th s-LNv), dorsal lateral neurons (LNds), lateral posterior neurons (LPNs), anterior dorsal neurons 1 (DN1as), posterior dorsal neurons 1 (DN1ps), dorsal neurons 2 (DN2s), and dorsal neurons 3 (DN3s) (Ahmad et al 2021 ; Crespo-Flores and Barber 2022 ). The s-LNv and l-LNv groups express a neuropeptide, Pigment-dispersing factor (PDF) (Helfrich-Förster 1995 ).…”

Section: Introductionmentioning

confidence: 99%

A four-oscillator model of seasonally adapted morning and evening activities in Drosophila melanogaster

2023

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The fruit fly Drosophila melanogaster exhibits two activity peaks, one in the morning and another in the evening. Because the two peaks change phase depending on the photoperiod they are exposed to, they are convenient for studying responses of the circadian clock to seasonal changes. To explain the phase determination of the two peaks, Drosophila researchers have employed the two-oscillator model, in which two oscillators control the two peaks. The two oscillators reside in different subsets of neurons in the brain, which express clock genes, the so-called clock neurons. However, the mechanism underlying the activity of the two peaks is complex and requires a new model for mechanistic exploration. Here, we hypothesize a four-oscillator model that controls the bimodal rhythms. The four oscillators that reside in different clock neurons regulate activity in the morning and evening and sleep during the midday and at night. In this way, bimodal rhythms are formed by interactions among the four oscillators (two activity and two sleep oscillators), which may judiciously explain the flexible waveform of activity rhythms under different photoperiod conditions. Although still hypothetical, this model would provide a new perspective on the seasonal adaptation of the two activity peaks.

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Integration of photoperiodic and temperature cues by the circadian clock to regulate insect seasonal adaptations

Hidalgo

Chiu

2023

J Comp Physiol A

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Organisms adapt to unfavorable seasonal conditions to survive. These seasonal adaptations rely on the correct interpretation of environmental cues such as photoperiod, and temperature. Genetic studies in several organisms, including the genetic powerhouse Drosophila melanogaster, indicate that circadian clock components, such as period and timeless, are involved in photoperiodic-dependent seasonal adaptations, but our understanding of this process is far from complete. In particular, the role of temperature as a key factor to complement photoperiodic response is not well understood. The development of new sequencing technologies has proven extremely useful in understanding the plastic changes that the clock and other cellular components undergo in different environmental conditions, including changes in gene expression and alternative splicing. This article discusses the integration of photoperiod and temperature for seasonal biology as well as downstream molecular and cellular pathways involved in the regulation of physiological adaptations that occur with changing seasons. We focus our discussion on the current understanding of the involvement of the molecular clock and the circadian clock neuronal circuits in these adaptations in D. melanogaster.

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The Drosophila circadian clock circuit is a nonhierarchical network of peptidergic oscillators

Cited by 5 publications

References 64 publications

The clock gene Cryptochrome 1 is involved in the photoresponse of embryonic hatching behavior in Bombyx mori

The clock gene Cryptochrome 1 is involved in the photoresponse of embryonic hatching behavior in Bombyx mori

A four-oscillator model of seasonally adapted morning and evening activities in Drosophila melanogaster

Integration of photoperiodic and temperature cues by the circadian clock to regulate insect seasonal adaptations

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