The Drosophila Claudin Kune-kune Is Required for Septate Junction Organization and Tracheal Tube Size Control

Nelson, Kevin S.; Furuse, Mikio; Beitel, Greg J.

doi:10.1534/genetics.110.114959

Cited by 102 publications

(115 citation statements)

References 40 publications

Supporting

Mentioning

110

Contrasting

Order By: Relevance

“…Although tight junction structures exist in some lower invertebrates and chordates, the equivalent structure in many invertebrate epithelia is located basal to the adherens junction, and is known as septate junction (e.g., insects), or the diffusion barrier may be part of adherens junctions (e.g., C. elegans) 171,172 . The septate junction in Drosophila contains claudin-like molecules that are important for barrier function [173][174][175][176][177] . C. elegans also expresses claudin-like molecules, and at least two of them are important for barrier formation; however, they are associated with adherens junctions 178 .…”

Section: Box 1 -Evolutionary Conservation Of Tight Junction Functionsmentioning

confidence: 99%

Tight junctions: from simple barriers to multifunctional molecular gates

Zihni

Mills

Matter

et al. 2016

Nat Rev Mol Cell Biol

1,145

925

View full text Add to dashboard Cite

Main body of text: 5983 words 2Epithelia and endothelia separate different tissue compartments and protect multicellular organisms form the outside world. This requires the formation of tight junctions, selective gates that control paracellular diffusion of ions and solutes. Tight junctions also form the border between the apical and basolateral plasma membrane domains and are linked to the machinery that controls apicobasal polarization. Additionally, signalling networks that guide diverse cell behaviours and functions are connected to tight junctions, transmitting information to and from the cytoskeleton, nucleus and different cell adhesion complexes. Here, we discuss recent advances in our understanding of the molecular architecture and cellular functions of tight junctions.Microscopists in the 19 th century described the paracellular space between neighbouring cells within an epithelial sheet to be sealed by a "terminal bar", a structure later resolved by electron microscopy into a composite of distinct cell-cell junctions that is now called the epithelial junctional complex and is formed by tight junctions, adherens junctions and desmosomes 1,2 . As the former two junctions are more tightly associated and often reside at the apical end of the lateral membrane, they are often referred to as the apical junctional complex (however, in endothelia, tight junctions and adherens junctions can be intercalated) (Fig. 1). Tight junctions are essential for barrier formation, and their primary physiological role is to function as paracellular gates that restrict diffusion on the basis of size and charge. Selective paracellular diffusion is an essential process for the maintenance of homoeostasis in organs and tissues. Tight junctions have long been the most enigmatic of all adhesion complexes and eluded a detailed molecular and functional analysis due to their complex architecture. Recent years have witnessed the identification of a large array of components associated with tight junctions implicating these junctions in an unexpected range of different functions, thereby challenging the traditional model, in which tight junctions are considered a simple diffusion barrier formed by a rigid molecular complex. In line with these various functions, mutations in genes encoding tight junction proteins have been linked to a range of inherited human diseases. Additionally, tight junction components are known to be targeted by a number of pathogenic bacteria and viruses, which hijack tight junction proteins to enter and infect cells, or target junctional signalling mechanisms to cross tissue barriers. Although tight junctions are a vertebrate junction, many of their components and functions are evolutionarily conserved (Box 1).The main purpose of this review is to examine the recent advances in the unravelling of the molecular architecture of tight junctions and understanding their functions. We will discuss recent exciting insights into how tight junctions function as signalling platforms that guide cell behaviour and differen...

show abstract

Section: Box 1 -Evolutionary Conservation Of Tight Junction Functionsmentioning

confidence: 99%

Tight junctions: from simple barriers to multifunctional molecular gates

Zihni

Mills

Matter

et al. 2016

Nat Rev Mol Cell Biol

1,145

925

View full text Add to dashboard Cite

show abstract

“…These cells remain in place until adult stages, and the septate junctions established remain stable over a very long time. Work on the tracheal system and the developing ectoderm has revealed a large number of genes required for the establishment of septate junctions (Fehon et al, 1994;Baumgartner et al, 1996;Behr et al, 2003;Genova and Fehon, 2003;Paul et al, 2003;Schulte et al, 2003;Faivre-Sarrailh et al, 2004;Llimargas et al, 2004;Wu et al, , 2007Banerjee et al, 2006a;Furuse and Tsukita, 2006;Hijazi et al, 2009;Nelson et al, 2010;Nilton et al, 2010). To date, ϳ18 cell-surface proteins have been described that are all structurally associated with septate junctions.…”

Section: Discussionmentioning

confidence: 99%

The CD59 Family Member Leaky/Coiled Is Required for the Establishment of the Blood–Brain Barrier inDrosophila

Syed¹,

Krudewig²,

Engelen³

et al. 2011

J. Neurosci.

View full text Add to dashboard Cite

The blood-brain barrier of Drosophila is established by the subperineurial glial cells that encase the CNS and PNS. The subperineurial glial cells are thin, highly interdigitated cells with epithelial character. The establishment of extensive septate junctions between these cells is crucial for the prevention of uncontrolled paracellular leakage of ions and solutes from the hemolymph into the nervous system. In the absence of septate junctions, macromolecules such as fluorescently labeled dextran can easily cross the blood-brain barrier. To identify additional components of the blood-brain barrier, we followed a genetic approach and injected Texas-Red-conjugated dextran into the hemolymph of embryos homozygous for chromosomal deficiencies. In this way, we identified the 153-aa-large protein Coiled, a new member of the Ly6 (leukocyte antigen 6) family, as being crucially required for septate junction formation and blood-brain barrier integrity. In coiled mutants, the normal distribution of septate junction markers such as NeurexinIV, Coracle, or Discs large is disturbed. EM analyses demonstrated that Coiled is required for the formation of septate junctions. We further show that Coiled is expressed by the subsperineurial glial cells in which it is anchored to the cell membrane via a glycosylphosphatidylinositol anchor and mediates adhesive properties. Clonal rescue studies indicate that the presence of Coiled is required symmetrically on both cells engaged in septate junction formation.

show abstract

“…Also has functional similarity because it localizes to and is required for the function of paracellular barrier junctions (Wu, et al, 2004). Kune Kune, this protein localizes to septate junctions and is required for junction organization and paracellular barrier function, but not for apical-basal polarity (Nelson, et al, 2010). In C. elegans g e n o m e d a t a b a s e i d e n t i f i e d f o u r c l a u d i nrelated, 20-kDa integral membrane proteins (CLC-1 to -4), which showed sequence similarity to the vertebrate claudins.…”

Section: Claudin Evolutionmentioning

confidence: 99%