The Drosophila homology of the mouse mammary oncogene int-1 is identical to the segment polarity gene wingless

Rijsewijk, F.A.M.; Schuermann, Marcus; Wagenaar, Els; Parren, Paul W.H.I.; Weigel, Detlef; Nusse, Roel

doi:10.1016/0092-8674(87)90038-9

Cited by 868 publications

(412 citation statements)

References 56 publications

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“…Since hh, arm and fu have phenotypes similar to wg with respect to their effects on en expression, it is tempting to speculate that some of these genes may encode other components involved in the transduction of the wg signal. The existence of genes in the mouse that are homologous to en 34 and wg 32 suggests that the circuitry proposed here for the control of en by wg may be phylogenetically conserved.…”

Section: Cell Communicationmentioning

confidence: 86%

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Two-tiered regulation of spatially patterned engrailed gene expression during Drosophila embryogenesis

DiNardo

Sher

Heemskerk-Jongens

et al. 1988

Nature

382

189

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A regulatory cascade, initiated during the syncytial stage of embryogenesis, culminates in the striped pattern of engrailed gene expression at the cellular blastoderm stage. The early regulatory genes, for example the pair-rule genes, are expressed transiently and as their products decay a distinct regulatory programme involving segment polarity genes takes over. This late programme maintains and perhaps modifies the striped pattern of engrailed expression through interactions that may involve cell communication.An early step in the generation of the Drosophila body pattern is the determination of the segmental subdivisions of the embryo. This occurs quite rapidly. By the cellular blastoderm stage (2-2.5 h after fertilization) the segmental outlines of the embryo, although not morphologically visible, are represented by the precise expression patterns of the segmentation (or segment polarity) genes, such as engrailed 1,2 and wingless 3 . The subsequent activity of genes such as these directs the morphological events leading to segmentation 4,5 .The segmentation genes are expressed in their localized patterns as a consequence of an early regulatory cascade. Three gene classes (maternal genes 6 , and the zygotically acting gap and pair-rule genes 4 ) act in sequence, each establishing the more refined spatial expression pattern of the next 7-9 . This takes place in a syncytial cytoplasm and is thought to rely on the diffusion and interaction of regulatory products encoded by these genes. The subdivision of the embryonic field by this cascade is rapid, occurring during the first thirteen nuclear divisions. After the thirteenth division, the nuclei are cellularized and the cellular blastoderm is formed.One outcome of this regulatory hierarchy is the establishment of engrailed (en) expression at the cellular blastoderm stage in 14 single-cell-wide stripes transecting the antero-posterior axis of the embryo 1,2,10,22 . Previous work has shown that the activity of separate sets of pair-rule genes controls establishment of en expression in even-and in odd-numbered stripes 11,12 . The data presented here suggests further that the separate control of even-and odd-numbered stripes is reflected in the organization of the cis-regulatory sequences at en.During the post-cellular blastoderm phase of development the expression pattern of en is maintained and modified 1,10 . It is unlikely that the pair-rule genes are involved in the postblastoderm regulation of en, since the pair-rule genes characterized to date are only expressed transiently in their pair-rule blastoderm patterns [13][14][15][16]31 . Here we address the mechanism by which the en gene is regulated in later embryonic development.

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Section: Cell Communicationmentioning

confidence: 86%

“…6). Similarities noted between the predicted Drosophila wg protein and the product of the mammalian oncogene int-1 have led to the suggestion that the wg product acts as a signal in cell communication 32 . This suggestion is consistent with genetic analysis showing that wg is not cell-autonomous 17,33 , 44 .…”

Section: Cell Communicationmentioning

confidence: 92%

Two-tiered regulation of spatially patterned engrailed gene expression during Drosophila embryogenesis

DiNardo

Sher

Heemskerk-Jongens

et al. 1988

Nature

382

189

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“…Probes used were: the 2 kbp ApaI/EcoRI fragment of the dpp E55 cDNA (Padgett et al, 1987), the 2.9 kbp lac2 fragment of pMC1871 (Casadaban et al, 1983), the 2 kbp EcoRVI HindIII fragment of wingless cDNA from pcDNA2 (Rijsewijk et al, 1987), the 1.1 kbp of twist cDNA (kindly provided by Dr. Chris Rushlow), and a 900 bp HindIII fragment plus the 800 bp HindIII-EcoRI fragment of the 5' P-element sequence from pPAl. Embryo preparation, hybridizations, and staining were as described (Tautz and Pfeiff le, 1989) except hybridizations were extended to 24-48 h at 48°C and post-hybridization washes were as follows: 12-24 h at 48°C in hybridization buffer with many changes followed by 6-12 h in PBS.…”

Section: Whole Mount In Situs Using Digoxigenin Labeled Probesmentioning

confidence: 99%

Embryonic expression patterns of the drosophila decapentaplegic gene: Separate regulatory elements control blastoderm expression and lateral ectodermal expression

Jackson

Hoffmann

1994

Developmental Dynamics

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Patterns of decapentaplegic (dpp) transcripts derived from the intact gene were compared to the patterns of transcripts generated by partial dpp transgenes in Drosophila embryos. Sequences closest to the dpp coding regions, the dpp hin region, were sufficient to express ZacZ-tagged mRNA in patterns indistinguishable from the patterns of endogenous dpp expression in the dorsal and terminal cells at the blastoderm stage, in the dorsal ectoderm during germ band elongation, and in narrow stripes of ectodermal cells along the dorsal edge of the ectoderm and at the boundary between the lateral and ventral neurogenic regions during germ band shortening. The latter pattern of expression responded to the segment polarity genes naked and wingless. However, these dpp sequences were not sufficient to drive ZucZ-tagged mRNA expression in other cells normally expressing dpp, including cells in the gnathal segments, the clypeolabrum, the foregut, the midgut visceral mesoderm, and the hindgut. Two separate regulatory regions were found in the dpp hin region. A 479 bp region upstream of the promoter was necessary for the segmented pattern of expression in the lateral ectoderm and for expression in the midgut endoderm. Cis-acting elements in the 2 kbp second intron directed expression in the dorsal and terminal regions of the blastoderm, acted on a heterologous promoter, the P-element promoter, and responded to pattern information derived from the maternal effect dorsal/ventral patterning genes. 0 1994 Wiley-Liss, Inc.

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“…Targeted disruption of the Wnt-1 gene revealed an essential role in development, as mouse embryos had severe defects in their midbrain and cerebellum (6)(7)(8). Wingless (Wg), the Drosophila homolog of Wnt-1, was independently identified as a segment polarity gene (9). Gene targeting of other Wnt genes demonstrated additional important roles for these molecules in kidney tubulogenesis and limb bud development (10,11).…”

mentioning

confidence: 99%

Purification and molecular cloning of a secreted, Frizzled-related antagonist of Wnt action

Kelley

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

384

306

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The Drosophila homology of the mouse mammary oncogene int-1 is identical to the segment polarity gene wingless

Cited by 868 publications

References 56 publications

Two-tiered regulation of spatially patterned engrailed gene expression during Drosophila embryogenesis

Two-tiered regulation of spatially patterned engrailed gene expression during Drosophila embryogenesis

Embryonic expression patterns of the drosophila decapentaplegic gene: Separate regulatory elements control blastoderm expression and lateral ectodermal expression

Purification and molecular cloning of a secreted, Frizzled-related antagonist of Wnt action

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