The drosophila sine oculis locus encodes a homeodomain-containing protein required for the development of the entire visual system

Cheyette, Benjamin N.R.; Green, Patricia J.; Martin, K.; Garren, Hideki; Hartenstein, Volker; Zipursky, S. Lawrence

doi:10.1016/0896-6273(94)90308-5

Cited by 494 publications

(435 citation statements)

References 52 publications

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“…The Six1 gene is homologous to Drosophila sine oculis (so) gene, an early regulator for Drosophila eye formation (Cheyette et al, 1994;Serikaku and O'Tousa, 1994). In Drosophila, so functions synergistically with the fly Pax6 gene eyeless (ey), eyes absent (eya) and dachshund (dac) to regulate the eye morphogenesis (reviewed by Treisman, 1999).…”

Section: Introductionmentioning

confidence: 99%

Six1is required for the early organogenesis of mammalian kidney

et al. 2003

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The murine Six gene family, homologous to Drosophila sine oculis(so) which encodes a homeodomain transcription factor, is composed of six members (Six1-6). Among the six members, only the Six2gene has been previously shown to be expressed early in kidney development,but its function is unknown. We have recently found that the Six1gene is also expressed in the kidney. In the developing kidney, Six1is expressed in the uninduced metanephric mesenchyme at E10.5 and in the induced mesenchyme around the ureteric bud at E11.5. At E17.5 to P0, Six1 expression became restricted to a subpopulation of collecting tubule epithelial cells. To study its in vivo function, we have recently generated Six1 mutant mice. Loss of Six1 leads to a failure of ureteric bud invasion into the mesenchyme and subsequent apoptosis of the mesenchyme. These results indicate that Six1 plays an essential role in early kidney development. In Six1-/- kidney development, we have found that Pax2, Six2 and Sall1expression was markedly reduced in the metanephric mesenchyme at E10.5,indicating that Six1 is required for the expression of these genes in the metanephric mesenchyme. In contrast, Eya1 expression was unaffected in Six1-/- metanephric mesenchyme at E10.5,indicating that Eya1 may function upstream of Six1. Moreover, our results show that both Eya1 and Six1expression in the metanephric mesenchyme is preserved in Pax2-/- embryos at E10.5, further indicating that Pax2 functions downstream of Eya1 and Six1 in the metanephric mesenchyme. Thus, the epistatic relationship between Pax, Eya and Six genes in the metanephric mesenchyme during early kidney development is distinct from a genetic pathway elucidated in the Drosophila eye imaginal disc. Finally, our results show that Eya1 and Six1genetically interact during mammalian kidney development, because most compound heterozygous embryos show hypoplastic kidneys. These analyses establish a role for Six1 in the initial inductive step for metanephric development.

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Section: Introductionmentioning

confidence: 99%

Six1is required for the early organogenesis of mammalian kidney

et al. 2003

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“…Genetic evidence strongly suggests that the RD factors are required, directly or indirectly, for transcriptional activation of ato. Several strong mutant alleles of the RD genes display 'eyeless' phenotypes in the adult and lack expression of ato in the L3 eye disc (Bonini et al, 1993;Cheyette et al, 1994;Jarman et al, 1995). Hence, we chose to investigate how the 3Јato 348 -␤gal construct responded to loss or gain of RD network function and assayed reporter gene expression in: (1) discs lacking the activity of the RD factors So or Eya; (2) discs in which Ey, or So and Eya, are ectopically expressed and induce ectopic eyes; and (3) eya or so mutant discs in which Ey is ectopically expressed but can not induce ectopic eyes.…”

Section: The Core Region Is Regulated By Rd Factorsmentioning

confidence: 99%

“…Other fly lines used: so 1 ; eya 2 ; UAS-ey; UAS-so; UAS-eya (Cheyette et al, 1994;Pignoni et al, 1997);and P{GAL4-dpp.blk1}40C.6 (FBti0002123).…”

Section: Research Articlementioning

confidence: 99%

Direct control of neurogenesis by selector factors in the fly eye:regulation ofatonalby Ey and So

et al. 2006

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During eye development, the selector factors of the Eyeless/Pax6 or Retinal Determination (RD) network control specification of organ-type whereas the bHLH-type proneural factor Atonal drives neurogenesis. Although significant progress has been made in dissecting the acquisition of 'eye identity' at the transcriptional level, the molecular mechanisms underlying the progression from neuronal progenitor to differentiating neuron remain unclear. A recently proposed model for the integration of organ specification and neurogenesis hypothesizes that atonal expression in the eye is RD-network-independent and that Eyeless works in parallel or downstream of atonal to modify the neurogenetic program. We show here that distinct cis-regulatory elements control atonal expression specifically in the eye and that the RD factors Eyeless and Sine oculis function as direct regulators. We find that these transcription factors interact in vitro and provide indirect evidence that this interaction may be required in vivo. The subordination of neurogenesis to the RD pathway in the eye provides a direct mechanism for the coordination of neurogenesis and tissue specification during sensory organ formation.

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“…Le premier membre de la famille Six a été isolé chez la drosophile, et baptisé sine oculis (so) car l'absence de ce gène dans les disques imaginaux oculaires entraîne un développement anormal de l'oeil [5,6]. So participe à l'organogenèse oculaire en collaboration avec les gènes eyeless (ey), eyes absent (eya) et dachshund (dac).…”

Section: Sine Oculisunclassified

Redéploiement des gènes Six au cours de l’évolution

Laclef

Maire

2004

Med Sci (Paris)

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The drosophila sine oculis locus encodes a homeodomain-containing protein required for the development of the entire visual system

Cited by 494 publications

References 52 publications

Six1is required for the early organogenesis of mammalian kidney

Six1is required for the early organogenesis of mammalian kidney

Direct control of neurogenesis by selector factors in the fly eye:regulation ofatonalby Ey and So

Redéploiement des gènes Six au cours de l’évolution

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