The Drug-Loaded Electrospun Poly(ε-Caprolactone) Mats for Therapeutic Application

Abstract

Diclofenac sodium salt (DSS)-loaded electrospun nanofiber mats on the base of poly(ε-caprolactone) (PCL) were investigated as biocompatible nanofibrous mats for medical applications with the ability to inhibit bacterial infections. The paper presents the characteristics of fibrous mats made by electrospinning and determines the effect of medicament on the fiber morphology, chemical, mechanical and thermal properties, as well as wettability. PCL and DSS-loaded PCL nanofibrous mats were characterized using scanning electron microscopy, transmission electron microscopy, attenuated total reflectance-Fourier transform infrared spectrometry, dynamic mechanical analysis, and contact angle measurements. Electron paramagnetic resonance measurements confirmed the lifetime of DSS before and after application of high voltage during the electrospinning process. In vitro biocompatibility was studied, and it was proved to be of good viability with ~92% of the diploid human cells culture line composed of lung fibroblast (MRC 5) after 48 h of incubation. Moreover, the significant activity of DSS-loaded nanofibers against cancer cells, Ca Ski and HeLa, was established as well. It was shown that 12.5% (m/V) is the minimal concentration for antibacterial activity when more than 99% of Escherichia coli (Gram-negative) and 99% of Staphylococcus aureus (Gram-positive) have been exterminated.

Cefazolin Loaded Oxidized Regenerated Cellulose/Polycaprolactone Bilayered Composite for Use as Potential Antibacterial Dural Substitute

“…Cefazolin loaded PCL was weaker and less ductile after cefazolin loading since the tensile strength and strain at break decreased with increasing cefazolin concentration, while the tensile modulus was relatively unchanged ( Figure 3 b,d,f). This was consistent with the previous findings for other types of drug loaded PCL [ 35 , 36 , 37 , 38 ]. In contrast, tensile strength of cefazolin loaded ORC/PCL bilayered composite was unaffected, but the increase in tensile modulus and the decrease in strain at break with increasing cefazolin concentration were observed instead ( Figure 3 a,e).…”

Electrospun Nisin-Loaded Poly(ε-caprolactone)-Based Active Food Packaging

“…PCL has already been electrospun from benign or greener solvents. However, a small average diameter, smoothness, and uniform fibers could not be achieved [29][30][31][32].…”

“…However, due to the low boiling point and high vapor pressure of DCM, un-uniform and irregular electrospun fibers were observed [39,41,42]. Therefore, a sufficient ratio of the binary solvent system such as DCM/DMF could solve the issue [29]. The viscosities of the prepared solutions and the fibers' average diameters of all fibrous membranes are listed in Table 2.…”

Polymer-Drug Anti-Thrombogenic and Hemocompatible Coatings as Surface Modifications

“…These polymers can be designed to degrade at different rates depending on the desired time. Poly( L -lactide) (PLLA), poly( D , L -lactide) (PDLLA), poly(lactide- co -glycolide) (PLGA), polycaprolactone (PCL) [ 66 ], and polycarbonates have been extensively investigated as possible materials for the development of BRSs [ 67 , 68 ].…”