The Dual Function of Sugar Carriers: Transport and Sugar Sensing

Lalonde, Sylvie; Boles, Eckhard; Hellmann, Hanjo; Barker, Laurence; Patrick, John W.; Frommer, Wolf B; Ward, John M.

doi:10.2307/3870894

Cited by 150 publications

(197 citation statements)

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“…It is well known that sucrose supply increases the longevity of many cut flowers, since sucrose can act as a source of nutrition for tissues approaching carbohydrate starvation, flower opening and subsequent water relations (Kuiper et al, 1995), similar finding were obtained by Lalonde et al (1999);Nichols (1973); Ichimura (Ichimura, 1998) and Downs (1988).…”

Section: Discussionsupporting

confidence: 63%

Vase Life Extension of Rose Cut Flowers (Rosa Hybirida) as Influenced by Silver Nitrate and Sucrose Pulsing

Elgimabi¹

2011

American Journal of Agricultural and Biological Sciences

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Problem statement: This experiment was carried out in the laboratory of the Department of the Biology, Taif University, Saudi Arabia. Approach: The study was conducted to investigated the silver nitrate (AgNO 3 ) and sucrose at different concentration on rose cut flowers longevity, which determined on the basis of wilting, chlorophyll retention and carbohydrate degradation .The treatments were, water control, 20, 30 or 50 ppm AgNO 3 and sucrose at 1,2or 3% w/v. Results: The results showed, flower vase life was prolonged by all AgNO 3 treatments. The best concentration was 30 ppm .The effect was further improved when AgNO 3 was combined with 3% sucrose, which recorded the best vase life compared to other concentrations of sucrose .The per cent of wilting was minimized as result of using this combined treatment However the per cent of wilting increased with the increase in concentrations of AgNO 3 and complete wilting occurred after 10, 8 and 7 days when treated with 20, 30 and 50 ppm of AgNO 3 , respectively, while sucrose shortened the period to reach wilting. Also AgNO 3 at 30 ppm retarded the chlorophyll as well as carbohydrate degradation during the postharvest life. The experiments were repeated three times with three replicates and a completely randomized design had been used. The experiments extended from February, 2006-September, 2008. The difference between means was performed using Duncan multiple range test at 0.05 levels. Conclusion: A significant improvement in vase life of rose cut flowers was occurred when treated with 30 ppm (AgNO 3 ) more effects when combined with 3% (w/v) sucrose, so we recommended that to use the previous treatments with this concentrations for a long vase life and commercial productions.

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Section: Discussionsupporting

confidence: 63%

Vase Life Extension of Rose Cut Flowers (Rosa Hybirida) as Influenced by Silver Nitrate and Sucrose Pulsing

Elgimabi¹

2011

American Journal of Agricultural and Biological Sciences

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“…For example, in Saccharomyces cerevisiae, MEP2 is a member of the ammonium permeases and serves as both a high-affinity ammonium transporter and a sensor (Lorenz and Heitman, 1998). A member of the sugar transporter family has also been proposed to have a dual function as a transporter and a sensor in yeast and plants (reviewed in Lalonde et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

The Chlamydomonas reinhardtii Nar1 Gene Encodes a Chloroplast Membrane Protein Involved in Nitrite Transport

2000

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A key step for nitrate assimilation in photosynthetic eukaryotes occurs within chloroplasts, where nitrite is reduced to ammonium, which is incorporated into carbon skeletons. The Nar1 gene from Chlamydomonas reinhardtii is clustered with five other genes for nitrate assimilation, all of them regulated by nitrate. Sequence analysis of genomic DNA and cDNA of Nar1 and comparative studies of strains having or lacking Nar1 have been performed. The deduced amino acid sequence indicates that Nar1 encodes a chloroplast membrane protein with substantial identity to putative formate and nitrite transporters in bacteria. Use of antibodies against NAR1 has corroborated its location in the plastidic membrane. Characterization of strains having or lacking this gene suggests that NAR1 is involved in nitrite transport in plastids, which is critical for cell survival under limiting nitrate conditions, and controls the amount of nitrate incorporated by the cells under limiting CO 2 conditions.

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“…In general, ethanol fermentation under aerobic conditions occurs mainly due to high glycolytic fluxes and/or limited oxygen availability, where the excess pyruvate that cannot be oxidized is fermented to regenerate the NAD + lost in glycolysis. It should be noted that glycolytic fluxes are regulated by the hexokinase activity in plants, which is determined by the supernatant hexose concentrations (Lalonde et al, 1999). Therefore, it is possible that the excessive hexose concentrations resulting from the Suc breakdown via SUS/invertase may have led to higher activity of hexokinase enzyme, thereby resulting in an overflow metabolism via the glycolytic pathway.…”

Section: Glycolysismentioning

confidence: 99%

Elucidating Rice Cell Metabolism under Flooding and Drought Stresses Using Flux-Based Modeling and Analysis

et al. 2013

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Rice (Oryza sativa) is one of the major food crops in world agriculture, especially in Asia. However, the possibility of subsequent occurrence of flood and drought is a major constraint to its production. Thus, the unique behavior of rice toward flooding and drought stresses has required special attention to understand its metabolic adaptations. However, despite several decades of research investigations, the cellular metabolism of rice remains largely unclear. In this study, in order to elucidate the physiological characteristics in response to such abiotic stresses, we reconstructed what is to our knowledge the first metabolic/regulatory network model of rice, representing two tissue types: germinating seeds and photorespiring leaves. The phenotypic behavior and metabolic states simulated by the model are highly consistent with our suspension culture experiments as well as previous reports. The in silico simulation results of seed-derived rice cells indicated (1) the characteristic metabolic utilization of glycolysis and ethanolic fermentation based on oxygen availability and (2) the efficient sucrose breakdown through sucrose synthase instead of invertase. Similarly, flux analysis on photorespiring leaf cells elucidated the crucial role of plastid-cytosol and mitochondrion-cytosol malate transporters in recycling the ammonia liberated during photorespiration and in exporting the excess redox cofactors, respectively. The model simulations also unraveled the essential role of mitochondrial respiration during drought stress. In the future, the combination of experimental and in silico analyses can serve as a promising approach to understand the complex metabolism of rice and potentially help in identifying engineering targets for improving its productivity as well as enabling stress tolerance.

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The Dual Function of Sugar Carriers: Transport and Sugar Sensing

Cited by 150 publications

References 0 publications

Vase Life Extension of Rose Cut Flowers (Rosa Hybirida) as Influenced by Silver Nitrate and Sucrose Pulsing

Vase Life Extension of Rose Cut Flowers (Rosa Hybirida) as Influenced by Silver Nitrate and Sucrose Pulsing

The Chlamydomonas reinhardtii Nar1 Gene Encodes a Chloroplast Membrane Protein Involved in Nitrite Transport

Elucidating Rice Cell Metabolism under Flooding and Drought Stresses Using Flux-Based Modeling and Analysis

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