The dynamic brain N-glycome

Lauc, Gordan

doi:10.1007/s10719-022-10055-x

Cited by 27 publications

(21 citation statements)

References 180 publications

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“…MALDI-TOF/TOF-MS/MS analysis revealed 145 distinct monosaccharide compositions which account for a total of 287 distinct N-glycan structures across all adult brain regions and species (Table S3), including those with distinctive S4) were ubiquitously present and their combined abundance disproportionately accounts for approximately 75% of the entire brain N-glycome. This conserved 'core network' (Fig 1B) predominantly consists of oligomannose and neutral fucosylated hybrid/complex structures supporting the assertion that although there is a tremendous amount of structural diversity among brain N-glycans (12), a relatively small number of simple N-glycans comprise the bulk of the brain N-glycome (15).…”

Section: Overview Of the Euarchontoglires Brain N-glycomesupporting

confidence: 59%

“…Asparagine-linked glycosylation (N-glycosylation) is critically involved in many aspects of central nervous system (CNS) biology including membrane excitability, synaptic transmission, neurite outgrowth, and neuronal plasticity ( 10 , 11 ). Recent insights have revealed that the brain N-glycome is dynamic and that its composition changes under various physiological and pathological conditions ( 12 ), yet data regarding its evolutionary trajectory are scarce. Only a single comparative N-glycomics study has investigated inter-species differences in N-glycosylation at the whole N-glycome level though it was limited to two species, mice and humans, and a single brain region ( 13 ).…”

Section: Main Textmentioning

confidence: 99%

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Human-specific features and developmental dynamics of the brain N-glycome

Gudelj

Santpere

Sousa

et al. 2023

Preprint

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Comparative "omics" studies have revealed unique aspects of human neurobiology, yet an evolutionary perspective of the brain N-glycome is lacking. Here, we performed multi-regional characterization of rat, macaque, chimpanzee, and human brain N-glycomes using chromatography and mass spectrometry, then integrated these data with complementary glycotranscriptomic data. We found that in primates the brain N-glycome has evolved more rapidly than the underlying transcriptomic framework, providing a mechanism for generating additional diversity. We show that brain N-glycome evolution in hominids has been characterized by an increase in complexity and α(2-6)-linked N-acetylneuraminic acid along with human-specific cell type expression of key glycogenes. Finally, by comparing the prenatal and adult human brain N-glycome, we identify region-specific neurodevelopmental pathways that lead to distinct spatial N-glycosylation profiles in the mature brain.

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Section: Overview Of the Euarchontoglires Brain N-glycomesupporting

confidence: 59%

Section: Main Textmentioning

confidence: 99%

Human-specific features and developmental dynamics of the brain N-glycome

Gudelj

Santpere

Sousa

et al. 2023

Preprint

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“…Brain N-glycosylation is known to be crucial for neurodevelopmental processes and for normal adult brain functions [47][48][49][50] and aberrant glycosylation often induces neurological disfunctions and is involved in other diseases like brain cancer 51 . The importance of glycosylation and the implications of aberrant glycosylation in this organ has been described by others 31,[52][53][54][55][56] and has recently been reviewed by Klaric et al 57 . Given the wealth of glycome information on this organ, we chose the brain to illustrate the advantages of isomer resolved PGC-LC-MS/MS data set and to demonstrate comparability with other studies.…”

Section: Resultsmentioning

confidence: 87%

Non-targeted isomer-sensitive N-glycome analysis reveals new layers of organ-specific diversity in mice

Helm

Mereiter

Oliveira

et al. 2022

Preprint

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Over 50% of all human proteins are post-translationally modified by glycans, which alter their functions in fundamental biological processes. Mice (mus musculus) represent the most common mammalian model organism to study fundamental biological processes, including glycosylation. Because of isomeric variance, the comprehensive analysis of protein-linked N-glycans is a challenging task. Coupled with high-resolution mass-spectrometry, porous graphitic carbon (PGC)-LC is a powerful and widely used method for isomer-specific glycan analysis. Here we report on a consistent and complete PGC-LC-MS/MS N-glycome dataset of 23 different mouse tissues, critically complementing existing glycan data-repositories. We used PGC-LC to chromatographically separate even closely related N-glycan isomers and an Orbitrap Exploris 480 mass-spectrometer for data-acquisition. Multivariate data analysis revealed tissue specific N-glycome signatures, highlighted organ-intrinsic regulations of glycobiological pathways and confirmed prior glycobiological knowledge, as exemplified by the brain N-glycome. This dataset can be used for fundamental glycobiological research, glycan analytical benchmarking, the development of new mass-spectrometric data analysis tools, glycobiological pathway modelling and simulation, as well as for integrative systems biology.

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“…Given the abundance of interactions with HS among axon guidance molecules and synaptic adhesion molecules as mentioned throughout this review, it is not surprising that HSPGs are involved in regulating major neurodevelopmental events 406‐408 . We cannot do justice to the functional roles of glycans in this review due to space, and therefore refer readers to other articles and reviews on the topic 407‐413 …”

Section: Other Wiring‐related Cell Surface Receptors and Ligandsmentioning

confidence: 99%

“… 406 , 407 , 408 We cannot do justice to the functional roles of glycans in this review due to space, and therefore refer readers to other articles and reviews on the topic. 407 , 408 , 409 , 410 , 411 , 412 , 413 …”

Section: Other Wiring‐related Cell Surface Receptors and Ligandsmentioning

confidence: 99%

Structure and evolution of neuronal wiring receptors and ligands

Cortés

Pak

Özkan

2022

Developmental Dynamics

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One of the fundamental properties of a neuronal circuit is the map of its connections. The cellular and developmental processes that allow for the growth of axons and dendrites, selection of synaptic targets, and formation of functional synapses use neuronal surface receptors and their interactions with other surface receptors, secreted ligands, and matrix molecules. Spatiotemporal regulation of the expression of these receptors and cues allows for specificity in the developmental pathways that wire stereotyped circuits. The families of molecules controlling axon guidance and synapse formation are generally conserved across animals, with some important exceptions, which have consequences for neuronal connectivity. Here, we summarize the distribution of such molecules across multiple taxa, with a focus on model organisms, evolutionary processes that led to the multitude of such molecules, and functional consequences for the diversification or loss of these receptors.

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The dynamic brain N-glycome

Cited by 27 publications

References 180 publications

Human-specific features and developmental dynamics of the brain N-glycome

Human-specific features and developmental dynamics of the brain N-glycome

Non-targeted isomer-sensitive N-glycome analysis reveals new layers of organ-specific diversity in mice

Structure and evolution of neuronal wiring receptors and ligands

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