The dynamic nature of exocytosis from large secretory vesicles. A view from electrochemistry and imaging

Borges, Ricardo; Gu, Chaoyi; Machado, José David; Ewing, Andrew G.

doi:10.1016/j.ceca.2023.102699

Cited by 13 publications

(3 citation statements)

References 89 publications

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“…Single-cell amperometry is used to detect exocytotic events and vesicular content at a single cell where the oxidation/reduction of a biomolecule at the electrode surface results in a transient spike in measured current . Ewing and co-workers have made substantial contributions to understanding vesicle dynamics, such as formation and exocytosis, by carefully piercing a cell membrane and rupturing vesicles to release stored biomolecules. − …”

Section: Single-cell Electroanalysismentioning

confidence: 99%

Recent Developments in Single-Entity Electrochemistry

Zhang,

Wahab,

Jallow

et al. 2024

Anal. Chem.

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rich approaches like SEE, is akin to swimming against the tide. A recent review from Long and co-workers gave persuasive views of new ways to think about data treatment in SEE, and interested readers are encouraged to consider the points raised. 268 Ultimately, SEE is well-positioned to advance further as measurement techniques improve in hardware, data treatment, and theory and as programming and the Internet of Things expand. We expect that interest in SEE will flourish as our science evolves.

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Section: Single-cell Electroanalysismentioning

confidence: 99%

Recent Developments in Single-Entity Electrochemistry

Zhang,

Wahab,

Jallow

et al. 2024

Anal. Chem.

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“…IVIEC, developed in 2015, enables direct quantification of the total neurotransmitter content within individual vesicles inside single cells . The combination of the two techniques shows partial release as the dominant mode of exocytosis. − Transmission electron microscopy (TEM) is a technique applied to visualize subcellular ultrastructures. Large dense-core vesicles (LDCVs) are one type of secretory vesicles that enclose a core of protein aggregates, which binds neurotransmitters and other small molecules .…”

Section: Introductionmentioning

confidence: 99%

Omega-3 and -6 Fatty Acids Alter the Membrane Lipid Composition and Vesicle Size to Regulate Exocytosis and Storage of Catecholamines

Gu,

Philipsen,

Ewing

2024

ACS Chem. Neurosci.

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The two essential fatty acids, alpha-linolenic acid and linoleic acid, and the higher unsaturated fatty acids synthesized from them are critical for the development and maintenance of normal brain functions. Deficiencies of these fatty acids have been shown to cause damage to the neuronal development, cognition, and locomotor function. We combined electrochemistry and imaging techniques to examine the effects of the two essential fatty acids on catecholamine release dynamics and the vesicle content as well as on the cell membrane phospholipid composition to understand how they impact exocytosis and by extension neurotransmission at the single-cell level. Incubation of either of the two fatty acids reduces the size of secretory vesicles and enables the incorporation of more double bonds into the cell membrane structure, resulting in higher membrane flexibility. This subsequently affects proteins regulating the dynamics of the exocytotic fusion pore and thereby affects exocytosis. Our data suggest a possible pathway whereby the two essential fatty acids affect the membrane structure to impact exocytosis and provide a potential treatment for diseases and impairments related to catecholamine signaling.

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“…Upon the completion of exocytosis, synaptic vesicles are recovered via endocytosis and replenished with neurotransmitters (4). Alternatively, vesicles are locally recycled (kiss-and-run) or refilled with neurotransmitters without undocking (kiss-and-stay) [14][15][16]. Upon the completion of exocytosis, synaptic vesicles are recovered via endocytosis and replenished with neurotransmitters (4).…”

Section: Introductionmentioning

confidence: 99%

Enhancing the Study of Quantal Exocytotic Events: Combining Diamond Multi-Electrode Arrays with Amperometric PEak Analysis (APE) an Automated Analysis Code

Tomagra,

Re,

Varzi

et al. 2023

Biosensors

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MicroGraphited-Diamond-Multi Electrode Arrays (μG-D-MEAs) can be successfully used to reveal, in real time, quantal exocytotic events occurring from many individual neurosecretory cells and/or from many neurons within a network. As μG-D-MEAs arrays are patterned with up to 16 sensing microelectrodes, each of them recording large amounts of data revealing the exocytotic activity, the aim of this work was to support an adequate analysis code to speed up the signal detection. The cutting-edge technology of microGraphited-Diamond-Multi Electrode Arrays (μG-D-MEAs) has been implemented with an automated analysis code (APE, Amperometric Peak Analysis) developed using Matlab R2022a software to provide easy and accurate detection of amperometric spike parameters, including the analysis of the pre-spike foot that sometimes precedes the complete fusion pore dilatation. Data have been acquired from cultured PC12 cells, either collecting events during spontaneous exocytosis or after L-DOPA incubation. Validation of the APE code was performed by comparing the acquired spike parameters with those obtained using Quanta Analysis (Igor macro) by Mosharov et al.

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The dynamic nature of exocytosis from large secretory vesicles. A view from electrochemistry and imaging

Cited by 13 publications

References 89 publications

Recent Developments in Single-Entity Electrochemistry

Recent Developments in Single-Entity Electrochemistry

Omega-3 and -6 Fatty Acids Alter the Membrane Lipid Composition and Vesicle Size to Regulate Exocytosis and Storage of Catecholamines

Enhancing the Study of Quantal Exocytotic Events: Combining Diamond Multi-Electrode Arrays with Amperometric PEak Analysis (APE) an Automated Analysis Code

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