The dynamic process of apoptosis analyzed by flow cytometry using Annexin-V/propidium iodide and a modified in situ end labeling technique

Span, L.F.R.; Pennings, A.H.M.; Vierwinden, G.; Boezeman, J.B.M.; Raymakers, R.A.P.; Witte, T. de

doi:10.1002/cyto.10028.abs

Cited by 12 publications

(16 citation statements)

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“…2F). Since phosphatidylserine translocation precedes oligo‐nucleosomal DNA‐fragmentation, after which nuclear membrane leakage occurs [27], it is evident that early apoptotic events are not involved in the nucleo‐cytoplasmatic translocation of PML. In summary, these results substantiate the observation that incoming HIV‐1 PICs trigger the accumulation of nuclear PML in speckled cytoplasmic structures, as previously shown [2].…”

Section: Resultsmentioning

confidence: 99%

Ini1/hSNF5 is dispensable for retrovirus‐induced cytoplasmic accumulation of PML and does not interfere with integration

Boese¹,

Sommer²,

Gaussin³

et al. 2004

FEBS Letters

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Retroviral infection triggers the cytoplasmic translocation of two Crm1-dependent shuttle factors, namely the Ini1 (integrase interactor 1, hSNF5) and the promyelocytic leukemia (PML) protein. Blocking nuclear export of shuttle factors by leptomycin B increases the efficiency of retroviral integration, suggesting that some may mediate antiviral activity. While PML was shown to counteract proviral establishment, it remained unclear whether Ini1, a protein implicated in various processes during human immunodeficiency virus replication, has the same potential. Employing RNA interference-mediated knockdown of Ini1, we show here that the simultaneous accumulation of both proteins in the cytoplasm likely reflects two non-interdependent phenomena. Furthermore, Ini1 does not interfere with retroviral integration, as cells lacking Ini1 show no increased infection susceptibility.

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Section: Resultsmentioning

confidence: 99%

Ini1/hSNF5 is dispensable for retrovirus‐induced cytoplasmic accumulation of PML and does not interfere with integration

Boese¹,

Sommer²,

Gaussin³

et al. 2004

FEBS Letters

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“…The results of CCK-8 assay correlated closely with the number of living cells according to the amount of an orange colored product (formazan) generated by dehydrogenases. However, Annexin V detects phosphatidylserine (PS) transposition on the outer plasma membrane which occurs early in the apoptotic process and precedes nuclear condensation, loss of membrane integrity, and cell shrinkage 20 . Therefore, there is only a correlation between CCK-8 and Annexin V assays, but the results may differ.…”

Section: Discussionmentioning

confidence: 99%

Ropivacaine- and bupivacaine-induced death of rabbit annulus fibrosus cells in vitro : involvement of the mitochondrial apoptotic pathway

Cai

Xia

Yang

et al. 2015

Osteoarthritis and Cartilage

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“…The annexin V assay detects early apoptosis. It has been suggested that cells that bind annexin V may at times not progress towards end-stage apoptosis but revert to a normal state [41,42]. We evaluated astrocyte apoptosis at 1, 5 and 10 days postinitiation of stimulation with L-OspA.…”

Section: Discussionmentioning

confidence: 99%

Pathogenesis of Lyme neuroborreliosis: Borrelia burgdorferi lipoproteins induce both proliferation and apoptosis in rhesus monkey astrocytes

Ramesh¹,

Alvarez²,

Roberts³

et al. 2003

Eur J Immunol

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Brain invasion by Borrelia burgdorferi, the agent of Lyme disease, results in an inflammatory and neurodegenerative disorder called neuroborreliosis. In humans, neuroborreliosis has been correlated with enhanced concentration of glial fibrillary acidic protein in the cerebrospinal fluid, a sign of astrogliosis. Rhesus monkeys infected by us with B. burgdorferi showed evidence of astrogliosis, namely astrocyte proliferation and apoptosis. We formulated the hypothesis that astrogliosis could be caused by spirochetal lipoproteins. We established primary cultures of rhesus monkey astrocytes and stimulated the cells with recombinant lipidated outer surface protein A (L-OspA), a model B. burgdorferi lipoprotein, and tripalmitoyl-S-glyceryl-Cys-Ser-Lys 4 -OH (Pam 3 Cys), a synthetic lipopeptide that mimics the structure of the lipoprotein lipid moiety. L-OspA elicited not only astrocyte proliferation but also apoptosis, two features observed during astrogliosis. Astrocytes produced both IL-6 and TNF- § in response to L-OspA and Pam 3 Cys. Proliferation induced by L-OspA was diminished in the presence of an excess of anti-IL-6 antibody, and apoptosis induced by this lipoprotein was completely suppressed with anti-TNF- § antibody. Hence, IL-6 contributes to, and TNF- § determines, astrocyte proliferation and apoptosis, respectively, as elicited by lipoproteins. Our results provide proof of the principle that spirochetal lipoproteins could be key virulence factors in Lyme neuroborreliosis, and that astrogliosis might contribute to neuroborreliosis pathogenesis.

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The dynamic process of apoptosis analyzed by flow cytometry using Annexin-V/propidium iodide and a modified in situ end labeling technique

Cited by 12 publications

References 0 publications

Ini1/hSNF5 is dispensable for retrovirus‐induced cytoplasmic accumulation of PML and does not interfere with integration

Ini1/hSNF5 is dispensable for retrovirus‐induced cytoplasmic accumulation of PML and does not interfere with integration

Ropivacaine- and bupivacaine-induced death of rabbit annulus fibrosus cells in vitro : involvement of the mitochondrial apoptotic pathway

Pathogenesis of Lyme neuroborreliosis: Borrelia burgdorferi lipoproteins induce both proliferation and apoptosis in rhesus monkey astrocytes

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