The E-Screen Assay: A Comparison of Different MCF7 Cell Stocks

Villalobos, Mercedes; Olea, Nicolás; Brotons, Jose Antonio; Olea-Serrano, Maria Fatima; Almodóvar, José Mariano Ruiz de; Pedraza, Vicente

doi:10.2307/3432398

Cited by 78 publications

(86 citation statements)

References 5 publications

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“…As such, this assay can be influenced by growth factors in addition to estrogen. Villalobos et al 16) investigated four different sublines of MCF-7 cells in an E-screen assay and found that MCF-7 BUS cells were more sensitive than MCF-7 cells from the American Type Culture Collection. Thus, it might be possible to obtain different results using different sublines of MCF-7 cells.…”

Section: Discussionmentioning

confidence: 99%

Cytocompatibility of a Tissue Conditioner Containing Vinyl Ester as a Plasticizer

et al. 2007

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In the current study, we examined the cytocompatibility of eight vinyl esters as candidate plasticizers for producing phthalate-and ethanol-free tissue conditioners. We measured the estrogenic activity and cytotoxicity of vinyl esters in human fibroblasts and keratinocytes using an E-screen assay and a mitochondrial dye conversion assay, respectively. We also assessed the cytotoxicity of three prototype materials and commercially available tissue conditioners on human fibroblasts grown in collagen gels. Finally, we measured the effects of these materials on the expression of cytokines in three-dimensional cultures by reverse transcriptase-polymerase chain reaction and enzyme-linked immunosorbent assays. None of the tested vinyl esters had estrogenic activity. Vinyl octanoate and vinyl pivalate were the least cytotoxic of the eight tested vinyl esters. In the same vein, a prototype tissue conditioner containing vinyl octanoate had equivalent or weaker cytotoxicity and induction of cytokine expression than conventional materials.

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Section: Discussionmentioning

confidence: 99%

Cytocompatibility of a Tissue Conditioner Containing Vinyl Ester as a Plasticizer

et al. 2007

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“…Very recently the design, synthesis and biological evaluation of a series of 2-and 6-disubstituted (RS)-9-(2,3-dihydro-1,4-benzoxathiin-3-ylmethyl)-9H-purine derivatives 70-80 were described [ Figure 5, Table 6. Antiproliferative activities against the MCF-7 cell line for 5-FU (Villalobos et al, 1995), and the six-membered alkylated purine derivatives.…”

Section: Synthesis and Anticancer Activity Of (Rs)-9-(23-dihydro-14mentioning

confidence: 99%

Benzo-Fused Seven- and Six-Membered Derivatives Linked to Pyrimidines or Purines Induce Apoptosis of Human Metastatic Breast Cancer MCF-7 Cells In Vitro

Campos¹,

Núñez²,

Conejo‐García³

et al. 2011

Breast Cancer - Current and Alternative Therapeutic Modalities

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“…Trypan blue dye exclusion was used to determine cell viability. The same experiment was performed using the sulphorodamine-B (SRB) colorimetric assay as described previously (Villalobos et al, 1995), with a Titertek Multiscan apparatus (Flow, Irvine, CA, USA) at 492 nm. We evaluated linearity of the colorimetric results with cell number for each MCF-7 cell stock before each cell growth experiment.…”

Section: Cell Proliferation Assaysmentioning

confidence: 99%

Inhibition of growth and induction of apoptosis in human breast cancer by transfection of gef gene

et al. 2003

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The gef gene has cell-killing functions in Escherichia coli. To evaluate the feasibility of using this gene as a new strategy for cancer therapy, we transfected it in MCF-7 cells derived from breast cancer (MCF-7TG). The gef gene was cloned in a pMAMneo vector under the control of a mouse mammary tumour virus promoter, inducible by dexamethasone (Dex), and was transfected with liposomes. After selection and induction, expression of the gef gene was confirmed by reverse transcription -polymerase chain reactions (RT -PCR) and Western blot. Cell viability was determined with a haemocytometre and the sulphorodamine B colorimetric assay, and the cell cycle was studied by propidium iodide (PI) staining. Annexin V-FITC and PI assays were used to evaluate apoptosis, which was confirmed by electron microscopy. In comparison with MCF-7 parental cells and MCF-7 cells transfected with an empty vector, MCF-7TG cells induced with Dex showed a significant decrease in proliferation rate, which was associated with evidence of apoptosis. Morphological findings confirmed apoptosis and showed a typical pattern of mitochondrial dilation. Furthermore, the cell cycle was characterised by premature progression from G 1 to S phase and G 2 delay. Our results show that the gef gene was able to decrease proliferation in a breast cancer cell line, and induce apoptosis. These findings suggest that the gef gene is a potential candidate for tumour therapy.

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The E-Screen Assay: A Comparison of Different MCF7 Cell Stocks

Cited by 78 publications

References 5 publications

Cytocompatibility of a Tissue Conditioner Containing Vinyl Ester as a Plasticizer

Cytocompatibility of a Tissue Conditioner Containing Vinyl Ester as a Plasticizer

Benzo-Fused Seven- and Six-Membered Derivatives Linked to Pyrimidines or Purines Induce Apoptosis of Human Metastatic Breast Cancer MCF-7 Cells In Vitro

Inhibition of growth and induction of apoptosis in human breast cancer by transfection of gef gene

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