The early extra petals1 Mutant Uncovers a Role for MicroRNA miR164c in Regulating Petal Number in Arabidopsis

Baker, Catherine; Sieber, Patrick; Wellmer, Frank; Meyerowitz, Elliot M.

doi:10.1016/j.cub.2005.02.017

Cited by 328 publications

(319 citation statements)

References 51 publications

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“…In inflorescences, miR164 targets CUC1/2 to regulate the establishment and maintenance of the shoot apical and axillary meristems and floral boundary formation (Laufs et al 2004; Mallory et al 2004; Baker et al 2005), whereas in root miR164 targets NAC1 to downregulate auxin signals for lateral root development (Guo et al 2005). Our sRNA sequencing and northern blot results confirmed that miR164 can be induced by V592-infection.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide profiling of sRNAs in the Verticillium dahliae-infected Arabidopsis roots

et al. 2018

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Small RNAs (sRNAs, including small interfering RNAs [siRNAs] and micro RNAs [miRNAs]) are key mediators of RNA silencing (or RNA interference), which play important roles in plant development and response to biotic and abiotic stimulation. Verticillium wilt is a plant vascular disease caused by the soil-borne fungal pathogens, such as Verticillium dahliae. We previously reported that V. dahliae infection increased two plant endogenous miRNAs that were exported to fungal cell to silence virulence genes. To investigate plant sRNAs in genome-wide response to V. dahliae infection, in this study, we constructed two sRNA libraries from Arabidopsis roots with and without V. dahliae infection, respectively. In total, 31 conserved miRNAs were found to be differentially expressed during the early stage of infection with V. dahliae using sRNA sequencing. Among these, the expression levels of miR160, miR164, miR166, miR167, miR390 and miR156h were confirmed by northern blot. Reverse transcription quantitative real time polymerase chain reaction results showed that the induction of miRNAs (miR160, miR164, miR166 and miR167) upon V. dahliae infection downregulated the expression of their targeted genes (ARF10, NAC1, PHV and ARF6), respectively. In addition, we identified specific phased siRNAs generated from distinct regions of two libraries. Profiling of these miRNAs and sRNAs lay the foundation for further understanding and utilising the host-induced gene silencing strategy to control plant vascular pathogens.

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Section: Discussionmentioning

confidence: 99%

Genome-wide profiling of sRNAs in the Verticillium dahliae-infected Arabidopsis roots

et al. 2018

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“…In animals, transcription studies of let-7 in C. elegans demonstrated that the temporal regulatory element (TRE) located at about 1200 nt upstream from the mature let-7 coding sequence was required for temporal upregulation of let-7 [45]. Recently, studies of the At-miR164 family showed that At-MIR164a and AtMIR164b participate in lateral root development [46], whereas At-MIR164c was demonstrated to control petal number in early flowers [47], indicating the nonredundant expression of the three members.…”

Section: Discussionmentioning

confidence: 99%

Duplication and expression analysis of multicopy miRNA gene family members in Arabidopsis and rice

Jiang

Yin

et al. 2006

Cell Res

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To understand the expansion of multicopy microRNA (miRNA) families in plants, we localized the reported miRNA genes from Arabidopsis and rice to their chromosomes, respectively, and observed that 37% of 117 miRNA genes from Arabidopsis and 35% of 173 miRNA genes from rice were segmental duplications in the genome. In order to characterize whether the expression diversification has occurred among plant multicopy miRNA family members, we designed PCR primers targeting 48 predicted miRNA precursors from 10 families in Arabidopsis and rice. Results from RT-PCR data suggest that the transcribed precursors of members within the same miRNA family were present at different expression levels. In addition, although miR160 and miR162 sequences were conserved in Arabidopsis and rice, we found that the expression patterns of these genes differed between the two species. These data suggested that expression diversification has occurred in multicopy miRNA families, increasing our understanding of the expression regulation of miRNAs in plants.

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“…Recent studies have uncovered the role of microRNAs (miRNAs), ~21-nucleotide noncoding RNAs, as regulators of floral patterning gene expression in Arabidopsis flower development (Aukerman and Sakai 2003;Schmid et al 2003;Achard et al 2004;Chen 2004;Mallory et al 2004a;Baker et al 2005;Millar and Gubler 2005). Plant miRNAs regulate cognate protein coding RNAs through either transcript cleavage or translational inhibition or both (reviewed in Bartel and Bartel 2003;Carrington and Ambros 2003;Bartel 2004;Dugas and Bartel 2004;He and Hannon 2004;Mallory and Vaucheret 2004;Kidner and Martienssen 2005;Chen 2005;Valencia-Sanchez et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Floral patterning defects induced by Arabidopsis APETALA2 and microRNA172 expression in Nicotiana benthamiana

et al. 2006

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Floral patterning and morphogenesis are controlled by many transcription factors including floral homeotic proteins, by which floral organ identity is determined. Recent studies have uncovered widespread regulation of transcription factors by microRNAs (miRNAs), ~21-nucleotide noncoding RNAs that regulate protein-coding RNAs through transcript cleavage and/or translational inhibition. The regulation of the floral homeotic gene APETALA2 (AP2) by miR172 is crucial for normal Arabidopsis flower development and is likely to be conserved across plant species. Here we probe the activity of the AP2/miR172 regulatory circuit in a heterologous Solanaceae species, Nicotiana benthamiana. We generated transgenic N. benthamiana lines expressing Arabidopsis wild type AP2 (35S∷AP2), miR172-resistant AP2 mutant (35S∷AP2m3) and MIR172a-1 (35S∷MIR172) under the control of the cauliflower mosaic virus 35S promoter. 35S∷AP2m3 plants accumulated high levels of AP2 mRNA and protein and exhibited floral patterning defects that included proliferation of numerous petals, stamens and carpels indicating loss of floral determinacy. On the other hand, nearly all 35S∷AP2 plants accumulated barely detectable levels of AP2 mRNA or protein and were essentially non-phenotypic. Overall, the data indicated that expression of the wild type Arabidopsis AP2 transgene was repressed at the mRNA level by an endogenous N. benthamiana miR172 homologue that could be detected using Arabidopsis miR172 probe. Interestingly, 35S∷MIR172 plants had sepal-to-petal transformations and/or more sepals and petals, suggesting interference with N. benthamiana normal floral homeotic gene function in perianth organs. Our studies uncover the potential utility of the Arabidopsis AP2/miR172 system as a tool for manipulation of floral architecture and flowering time in non-model plants.

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The early extra petals1 Mutant Uncovers a Role for MicroRNA miR164c in Regulating Petal Number in Arabidopsis

Abstract: The data presented in this study indicate that closely related miRNA family members that are predicted to target the same set of genes can have different functions during development, possibly because of nonoverlapping expression patterns.

Cited by 328 publications

References 51 publications

Genome-wide profiling of sRNAs in the Verticillium dahliae-infected Arabidopsis roots

Genome-wide profiling of sRNAs in the Verticillium dahliae-infected Arabidopsis roots

Duplication and expression analysis of multicopy miRNA gene family members in Arabidopsis and rice

Floral patterning defects induced by Arabidopsis APETALA2 and microRNA172 expression in Nicotiana benthamiana

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