The Ecdysone Receptor Coactivator Taiman Links Yorkie to Transcriptional Control of Germline Stem Cell Factors in Somatic Tissue

Zhang, Can; Robinson, Brian; Xu, Wenjian; Liu, Yang; Yao, Bing; Zhao, Heya; Byun, Phil K.; Jin, Peng; Veraksa, Alexey; Moberg, Kenneth H.

doi:10.1016/j.devcel.2015.05.010

Cited by 64 publications

(88 citation statements)

References 78 publications

(104 reference statements)

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“…Interestingly, nine of the genes down regulated by Hr4 also appear to be repressed by Tai according to our analysis, which suggests these two regulators could act as partners during border cell migration. Recent studies have also linked Tai with Yorkie signaling (Zhang et al, 2015), which is also active in border cell migration (Lin et al, 2014; Lucas et al, 2013), so it will be interesting to elucidate all of the pathways in which Tai acts.…”

Section: Discussionmentioning

confidence: 99%

A hormonal cue promotes timely follicle cell migration by modulating transcription profiles

Manning

Sheth

Bridges

et al. 2017

Mechanisms of Development

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Cell migration is essential during animal development. In the Drosophila ovary, the steroid hormone ecdysone coordinates nutrient sensing, growth, and the timing of morphogenesis events including border cell migration. To identify downstream effectors of ecdysone signaling, we profiled gene expression in wild-type follicle cells compared to cells expressing a dominant negative Ecdysone receptor or its coactivator Taiman. Of approximately 400 genes that showed differences in expression, we validated 16 candidate genes for expression in border and centripetal cells, and demonstrated that seven responded to ectopic ecdysone activation by changing their transcriptional levels. We found a requirement for seven putative targets in effective cell migration, including two other nuclear hormone receptors, a calcyphosine-encoding gene, and a prolyl hydroxylase. Thus, we identified multiple new genetic regulators modulated at the level of transcription that allow cells to interpret information from the environment and coordinate cell migration in vivo.

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Section: Discussionmentioning

confidence: 99%

A hormonal cue promotes timely follicle cell migration by modulating transcription profiles

Manning

Sheth

Bridges

et al. 2017

Mechanisms of Development

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“…Finally, ecdysone signaling impinges on another critical growth, survival and proliferation pathway in the wing, the Hippo signaling pathway (Saucedo and Edgar, 2007). An EcR co-activator Taiman (Tai) binds to the downstream Hippo pathway transcription factor Yorkie, and is also required for normal proliferation in the larval wing pouch (Zhang et al, 2015). Thus, in the larval stages where wing cells are largely asynchronously proliferating, ecdysone signaling is required to promote proliferation and growth.…”

Section: Introductionmentioning

confidence: 99%

Ecdysone signaling induces two phases of cell cycle exit inDrosophilacells

et al. 2016

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During development, cell proliferation and differentiation must be tightly coordinated to ensure proper tissue morphogenesis. Because steroid hormones are central regulators of developmental timing, understanding the links between steroid hormone signaling and cell proliferation is crucial to understanding the molecular basis of morphogenesis. Here we examined the mechanism by which the steroid hormone ecdysone regulates the cell cycle in Drosophila. We find that a cell cycle arrest induced by ecdysone in Drosophila cell culture is analogous to a G2 cell cycle arrest observed in the early pupa wing. We show that in the wing, ecdysone signaling at the larva-to-puparium transition induces Broad which in turn represses the cdc25c phosphatase String. The repression of String generates a temporary G2 arrest that synchronizes the cell cycle in the wing epithelium during early pupa wing elongation and flattening. As ecdysone levels decline after the larva-to-puparium pulse during early metamorphosis, Broad expression plummets, allowing String to become re-activated, which promotes rapid G2/M progression and a subsequent synchronized final cell cycle in the wing. In this manner, pulses of ecdysone can both synchronize the final cell cycle and promote the coordinated acquisition of terminal differentiation characteristics in the wing.

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“…In the nucleus, Yki normally cooperates with other transcription factors to promote the expression of genes that enhance growth (e.g., cyclin E) and survival (e.g., DIAP-1 and bantam). A causal role for the altered expression of SWH pathway targets in l(3) mbt mutant tumors is supported by studies in which mutation of bantam or yki or enforced expression of Ex suppressed tumorigenesis (Richter et al 2011;Blanchard et al 2014;Zhang et al 2015).…”

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confidence: 68%

“…This may be attributable to the alteration of the SWH pathway, as Piwi and Ago3 become expressed in hyperactive Yki wing disc cells. This suggests that transcription factors controlled by Yki can directly or indirectly contribute to the regulation of germline gene expression programs (Polesello and Tapon 2007;Blanchard et al 2014;Zhang et al 2015). Among the reactivated germline genes are also components of secondary biogenesis pathways, including Aub and Ago3.…”

Section: Discussionmentioning

confidence: 99%

“…This could be due to the fact that germline transcription factors, which are targets of l(3)mbt, also become activated (Richter et al 2011;Meier et al 2012;Blanchard et al 2014;Zhang et al 2015). These include LINT, which has been implicated in the regulation of Piwi expression, as well as vasa (vas), bag of marbles (bam), and benign gonial cell neoplasm (bgcn), all of which play important roles in the regulation of germ cell division.…”

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confidence: 99%

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Oncogenic transformation of Drosophila somatic cells induces a functional piRNA pathway

et al. 2016

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Germline genes often become re-expressed in soma-derived human cancers as “cancer/testis antigens” (CTAs), and piRNA (PIWI-interacting RNA) pathway proteins are found among CTAs. However, whether and how the piRNA pathway contributes to oncogenesis in human neoplasms remain poorly understood. We found that oncogenic Ras combined with loss of the Hippo tumor suppressor pathway reactivates a primary piRNA pathway in Drosophila somatic cells coincident with oncogenic transformation. In these cells, Piwi becomes loaded with piRNAs derived from annotated generative loci, which are normally restricted to either the germline or the somatic follicle cells. Negating the pathway leads to increases in the expression of a wide variety of transposons and also altered expression of some protein-coding genes. This correlates with a reduction in the proliferation of the transformed cells in culture, suggesting that, at least in this context, the piRNA pathway may play a functional role in cancer.

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The Ecdysone Receptor Coactivator Taiman Links Yorkie to Transcriptional Control of Germline Stem Cell Factors in Somatic Tissue

Cited by 64 publications

References 78 publications

A hormonal cue promotes timely follicle cell migration by modulating transcription profiles

A hormonal cue promotes timely follicle cell migration by modulating transcription profiles

Ecdysone signaling induces two phases of cell cycle exit inDrosophilacells

Oncogenic transformation of Drosophila somatic cells induces a functional piRNA pathway

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