The ecological and physiological responses of the microbial community from a semiarid soil to hydrocarbon contamination and its bioremediation using compost amendment

Bastida, Felipe; Jehmlich, Nico; Lima, Kelly Pereira de; Morris, Brandon E. L.; Richnow, Hans H.; Hernández, Teresa; Bergen, Martin von; Garcı́a, Carlos

doi:10.1016/j.jprot.2015.07.023

Cited by 130 publications

(66 citation statements)

References 60 publications

(82 reference statements)

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“…After electrophoresis, the gels were stained with colloidal Coomassie brilliant blue (Bastida et al . ). The gel area containing the protein mixture of each sample was sliced into one piece.…”

Section: Methodsmentioning

confidence: 97%

The active microbial diversity drives ecosystem multifunctionality and is physiologically related to carbon availability in Mediterranean semi‐arid soils

et al. 2016

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Biogeochemical processes and ecosystemic functions are mostly driven by soil microbial communities. However, most methods focus on evaluating the total microbial community and fail to discriminate its active fraction which is linked to soil functionality. Precisely, the activity of the microbial community is strongly limited by the availability of organic carbon (C) in soils under arid and semi-arid climate. Here, we provide a complementary genomic and metaproteomic approach to investigate the relationships between the diversity of the total community, the active diversity and ecosystem functionality across a dissolved organic carbon (DOC) gradient in southeast Spain. DOC correlated with the ecosystem multifunctionality index composed by soil respiration, enzyme activities (urease, alkaline phosphatase and β-glucosidase) and microbial biomass (phospholipid fatty acids, PLFA). This study highlights that the active diversity (determined by metaprotoemics) but not the diversity of the whole microbial community (evaluated by amplicon gene sequencing) is related to the availability of organic C and it is also connected to the ecosystem multifunctionality index. We reveal that DOC shapes the activities of bacterial and fungal populations in Mediterranean semi-arid soils and determines the compartmentalization of functional niches. For instance, Rhizobales thrived at high-DOC sites probably fuelled by metabolism of one-C compounds. Moreover, the analysis of proteins involved in the transport and metabolism of carbohydrates revealed that Ascomycota and Basidiomycota occupied different nutritional niches. The functional mechanisms for niche specialization were not constant across the DOC gradient.

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Section: Methodsmentioning

confidence: 97%

The active microbial diversity drives ecosystem multifunctionality and is physiologically related to carbon availability in Mediterranean semi‐arid soils

et al. 2016

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“…In this study, both Grampositive and -negative bacteria were isolated at same frequency (50%) from sample collected before and during remediation. Recently, Bastida et al (2016) reported that the resistance mechanism of Gram-negative bacteria to hydrocarbon was attributed to membrane modification (variations in membrane fluidity), whilst that of Grampositive was likely due to changes in biomass. Similarly, Lazaroaie (2010) attributed the ability of Gram-negative bacteria group to withstand pollutant in soil, especially PAH, to cell wall composition of this bacterial group.…”

Section: Discussionmentioning

confidence: 99%

Shift in microbial group during remediation by enhanced natural attenuation (RENA) of a crude oil-impacted soil: a case study of Ikarama Community, Bayelsa, Nigeria

2017

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Acute and chronic pollution of environments with crude oil does not bode well for biota living within the vicinity of polluted environments. This is due to environmental and public health concerns on the negative impact of crude oil pollution on living organisms. Enhancing microbial activities by adding nutrients and other amendments had proved effective in pollutant removal during bioremediation. This study was carried out to determine how microbial group respond during remediation by enhanced natural attenuation (RENA) during a field-scale bioremediation. Crude oil-polluted soil samples were collected (before, during, and after remediation) from a site undergoing remediation by enhanced natural attenuation (RENA) at Ikarama Community, Bayelsa State, Nigeria, and were analyzed for total petroleum hydrocarbon (TPH), polyaromatic hydrocarbon (PAH), and a shift in microbial community. The gas chromatography-flame ionization detector (GC-FID) results showed that the pollutant concentrations (TPH and PAH) reduced by 98 and 85%, respectively, after the remediation. Culturable hydrocarbon utilizing bacteria (CHUB) was highest (8.3 9 10 4 cfu/g) for sample collected during the remediation studies, whilst sample collected after remediation had low CHUB (6

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“…TPH levels in soil were found decreased when incubation time increased. Bastida et al, (2016) reported that the long-term respiration was lower in the contaminated samples in comparison to controls, probably as a consequence of extended exposure of microorganisms to pollutants.…”

Section: Total Petroleum Hydrocarbonsmentioning

confidence: 97%

“…β-Glucosidase activity (BGA) in soil might be particularly useful for soil quality monitoring because of the central role of this enzyme in soil organic matter cycling, which is generally regarded as an important component of soil quality (Turner et al, 2002) In the present study, crude oil contamination did not affected negatively. The contamination with crude oil increased the amount of total organic and water-soluble C. Some compounds within these fractions may serve as energy sources for microbial populations (Bastida et al, 2016). However, the level of BGA in Effect of wasterwater sludge application on enzyme activities in soil (Kaczyńska et al, 2015).…”

Section: Soil Enzyme Activitiesmentioning

confidence: 99%

Effect of wasterwater sludge application on enzyme activities in soil contaminated with crude oil

Dindar

Topaç

Başkaya

et al. 2017

J. Soil Sci. Plant Nutr.

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Crude oil affects soil ecosystems, resulting in significant losses in soil quality. The objective of the present study was to evaluate the effects of hydrocarbon pollution (crude oil) on soil enzyme activities and determine the removal of total petroleum hydrocarbons (TPH) during an incubation period of 150 days. The potential use of wastewater sludge as a biostimulating agent in petroleum-contaminated soils was also evaluated. The degradation of crude oil in contaminated soil was significantly enhanced after the addition of wastewater sludge.The results also indicated that significant TPH degradation in crude oil-contaminated soils (80% for dose of 0.5% and 83% for dose of 5% at 18 °C) (94% for dose of 0.5% and 92% for dose of 5% at 28 °C) occurred after an incubation period of 150 days. Enhanced enzyme activity levels in contaminated soils indicated that crude oil (0.5% and 5%, w/w) stimulated microbial growth and enzyme activity in the soil environment. Urease activity (UA) levels in crude oil contaminated soils were generally higher than initial UA levels in soil during the incubation period. Soil UA exhibited a stronger response to petroleum treatment. Alkaline phosphatase (APA) and β-Glucosidase (BGA) activities were not affected crude oil pollution. However, contamination of crude oil had a negatively effect on soil dehydrogenase activity (DHA).

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The ecological and physiological responses of the microbial community from a semiarid soil to hydrocarbon contamination and its bioremediation using compost amendment

Cited by 130 publications

References 60 publications

The active microbial diversity drives ecosystem multifunctionality and is physiologically related to carbon availability in Mediterranean semi‐arid soils

The active microbial diversity drives ecosystem multifunctionality and is physiologically related to carbon availability in Mediterranean semi‐arid soils

Shift in microbial group during remediation by enhanced natural attenuation (RENA) of a crude oil-impacted soil: a case study of Ikarama Community, Bayelsa, Nigeria

Effect of wasterwater sludge application on enzyme activities in soil contaminated with crude oil

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