Generation of reactive oxygen species (ROS) by common
sunscreen
agents such as titanium dioxide (TiO2) and zinc oxide (ZnO)
has prompted much research to incorporate effective antioxidants into
sunscreens. Nanodiamonds (NDs) and fullerenes (C60) are
potent antioxidants (radical scavengers) and effective UV filters,
making them prime candidates as additives. The radical scavenging
potentials of NDs and C60 are compared to the common antioxidant
vitamin C using the 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH)
assay. In the presence of UV irradiation and ROS-generating TiO2 nanoparticles, we found that the radical scavenging potentials
of vitamin C significantly decrease, while those of NDs and C60 are enhanced. This suggests that NDs and C60 can
be used as long-lasting antioxidants to complement sunscreens. NDs
and C60 are further incorporated into prototype creams
along with TiO2 nanoparticles. When these prototype creams
are subjected to simulated solar irradiation, the transmission spectra
show that the anti-UV properties of the creams are well maintained
after prolonged irradiation. Hence, NDs and C60 are photostable
UV filters, which can supplement the sun protection efficacy of sunscreens.
Cell studies using human dermal fibroblasts are also conducted to
demonstrate the ability of NDs and C60 to scavenge the
intracellular ROS generated by TiO2 nanoparticles. Under
irradiation, ND significantly reduces intracellular oxidative stress.
This is not observed for C60 because it can behave as both
a ROS generator and scavenger when irradiated. This work establishes
the antioxidant and anti-UV benefits of adding NDs and C60 to sunscreens.