The ecotoxicological contaminant tris(4-chlorophenyl)methanol (TCPMOH) impacts embryonic development in zebrafish (Danio rerio)

Navarrete, Julian; Wilson, Peyton W; Allsing, Nicholas; Gordon, Chandi; Margolis, Rachel; Schwartz, Ashley V.; Cho, Christine; Rogowski, Brynn; Topps, Jennifer; George, Uduak Z.; Sant, Karilyn E.

doi:10.1016/j.aquatox.2021.105815

Cited by 8 publications

(19 citation statements)

References 45 publications

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“…Therefore, these various TCPM/TCPMOH isomers may be markers of historic DDT pollution. Understanding TCPM/TCPMOH contamination is important because they biomagnify, are highly persistent, and can be acutely toxic. , …”

Section: Results and Discussionmentioning

confidence: 99%

“…Understanding TCPM/ TCPMOH contamination is important because they biomagnify, are highly persistent, and can be acutely toxic. 48,49 Estrogen Activation Assays. Potential activation of California condor ERα and ERβ by TCPM, TCPMOH, and PCBs 101, 110, 118, 170, 183, and 187 was assessed by methods described previously (SI Figure 4).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Assessing Marine Endocrine-Disrupting Chemicals in the Critically Endangered California Condor: Implications for Reintroduction to Coastal Environments

Stack

Cossaboon

Tubbs³

et al. 2022

Environ. Sci. Technol.

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Coastal reintroduction sites for California condors (Gymnogyps californianus) can lead to elevated halogenated organic compound (HOC) exposure and potential health impacts due to the consumption of scavenged marine mammals. Using nontargeted analysis based on comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry (GC×GC/TOF-MS), we compared HOC profiles of plasma from inland and coastal scavenging California condors from the state of California (CA), and marine mammal blubber from CA and the Gulf of California off Baja California (BC), Mexico. We detected more HOCs in coastal condors (32 ± 5, mean number of HOCs ± SD, n = 7) than in inland condors (8 ± 1, n = 10) and in CA marine mammals (136 ± 87, n = 25) than in BC marine mammals (55 ± 46, n = 8). ∑DDT-related compounds, ∑PCBs, and total tris(chlorophenyl)methane (∑TCPM) were, respectively, ∼7, ∼3.5, and ∼148 times more abundant in CA than in BC marine mammals. The endocrine-disrupting potential of selected polychlorinated biphenyls (PCB) congeners, TCPM, and TCPMOH was determined by in vitro California condor estrogen receptor (ER) activation. The higher levels of HOCs in coastal condors compared to those in inland condors and lower levels of HOC contamination in Baja California marine mammals compared to those from the state of California are factors to consider in condor reintroduction efforts.

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Section: Results and Discussionmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Assessing Marine Endocrine-Disrupting Chemicals in the Critically Endangered California Condor: Implications for Reintroduction to Coastal Environments

Stack

Cossaboon

Tubbs³

et al. 2022

Environ. Sci. Technol.

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“…Recent evidence suggests chemical exposures during sensitive time periods may contribute to the developmental origins of diabetes by disrupting organogenesis of the developing pancreas (Inadera, 2013;Sant, Jacobs, et al, 2017;Simmons, 2006Simmons, , 2007. Developmental exposures to TCPM and TCPMOH have been shown to induce embryonic mortality and deformities such as pericardial and yolk sac edema in zebrafish embryos (Navarrete et al, 2021;Yost et al, 2022). In the present study, we aimed to characterize the developmental toxicity of TCPM and TCPMOH to the zebrafish developing pancreas and gene expression.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, these effects of organohalogens on the developing pancreas are modulated and often exacerbated in models prone to oxidative stress, including Nrf2-deficient zebrafish embryos (Jacobs et al, 2018;. Because we have previously shown that TCPMOH impairs gene expression within the Nrf2 antioxidant pathway in zebrafish embryos (Navarrete et al, 2021), this suggests that these TCPMOH and potentially TCPMexposed embryos may be especially sensitive to oxidative stress and oxidative damage in the pancreas.…”

Section: Introductionmentioning

confidence: 99%

Tris(4‐chlorophenyl)methane and tris(4‐chlorophenyl)methanol disrupt pancreatic organogenesis and gene expression in zebrafish embryos

Wilson

Cho

Allsing

et al. 2022

Birth Defects Research

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Objectives Tris(4‐chlorophenyl) methane (TCPM) and tris(4‐chlorophenyl)methanol (TCPMOH) are anthropogenic environmental contaminants believed to be manufacturing byproducts of the organochlorine pesticide dichlorodiphenyltrichloroethane (DDT) due to environmental co‐occurrence. TCPM and TCPMOH are persistent, bioaccumulate in the environment, and are detected in human breast milk and adipose tissues. DDT exposures have been previously shown to disrupt insulin signaling and glucoregulation, increasing risk for diabetes. We have previously shown that embryonic exposures organochlorines such as polychlorinated biphenyls disrupted pancreatic development and early embryonic glucoregulatory networks. Here, we determined the impacts of the similar compounds TCPM and TCPMOH on zebrafish pancreatic growth and gene expression following developmental exposures. Methods Zebrafish embryos were exposed to 50 nM TCPM or TCPMOH beginning at 24 hr postfertilization (hpf) and exposures were refreshed daily. At 96 hpf, pancreatic growth and islet area were directly visualized in Tg(ptf1a::GFP) and Tg(insulin::GFP) embryos, respectively, using microscopy. Gene expression was assessed at 100 hpf with RNA sequencing. Results Islet and total pancreas area were reduced by 20.8% and 13% in embryos exposed to 50 nM TCPMOH compared to controls. TCPM did not induce significant morphological changes to the developing pancreas, indicating TCPMOH, but not TCPM, impairs pancreatic development despite similarity in molecular responses. Transcriptomic responses to TCPM and TCPMOH were correlated (R2 = .903), and pathway analysis found downregulation of processes including retinol metabolism, circadian rhythm, and steroid biosynthesis. Conclusion Overall, our data suggest that TCPM and TCPMOH may be hazardous to embryonic growth and development.

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“…LC 50 values for 24 hours, 48 hours, 72 hours, and 96 hours and the LT 50 values for each calculable concentration, within the 95% confidence limits, were determined using the Probit Regression analysis using IBM SPSS Statistics version 25.0 for Windows package software (IBM Corp., Armonk, NY, USA) (Lei and Sun, 2018;Navarrete et al, 2021).…”

Section: Statistical Analysesmentioning

confidence: 99%

Acute Toxicity of Zinc on Southern Medicinal Leech, Hirudo verbana Carena, 1820

Ceylan¹,

Çetinkaya²,

Bulut³

2021

Acta Aquatica Turcica

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In the present study it was aimed to determine the acute toxicity of zinc on southern medicinal leech Hirudo verbana Carena, 1820 that being used in the ecotoxicological studies. The experiment was conducted for 96 hours using the semi-static bioassay method with replicate two times. The leeches were exposed to zinc concentrations of 6.25 mg/L, 12.50 mg/L, 25 mg/L, 50 mg/L, 100 mg/L and 200 mg/L, respectively. As a result of the experiment, the LC50 values for 24, 48, 72 and 96 hours were determined 48.30 mg/L, 19.68 mg/L, 15.48 mg/L, and 14.12 mg/L, respectively. The LT50 values for 25 mg/L, 50 mg/L, 100 mg/L and 200 mg/L zinc concentrations were determined 38.63 hours, 24.55 hours, 14.62 hours, and 12.78 hours, respectively. Defecation, intensive mucus output, uncontrolled swimming, attempt to escape from the experiment media, reducing the cling power, coiling (buckling), vomiting the digestive blood, the body deformations, penile prolapse, hemorrhage and abrasion in the wall of the body were the main physiological and behavioral responses in the leeches which were exposed to the different zinc solutions. The data obtained revealed that the zinc sensitivity of H. verbana was moderate when compared to other organisms. It is expected that the data of the present study will contribute to the management of endangered medicinal leech populations.

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The ecotoxicological contaminant tris(4-chlorophenyl)methanol (TCPMOH) impacts embryonic development in zebrafish (Danio rerio)

Cited by 8 publications

References 45 publications

Assessing Marine Endocrine-Disrupting Chemicals in the Critically Endangered California Condor: Implications for Reintroduction to Coastal Environments

Assessing Marine Endocrine-Disrupting Chemicals in the Critically Endangered California Condor: Implications for Reintroduction to Coastal Environments

Tris(4‐chlorophenyl)methane and tris(4‐chlorophenyl)methanol disrupt pancreatic organogenesis and gene expression in zebrafish embryos

Acute Toxicity of Zinc on Southern Medicinal Leech, Hirudo verbana Carena, 1820

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