The Ectopic Expression of Btr2 in Aegilops tauschii Switches the Disarticulation Layer From Above to Below the Rachis Node

Zeng, Xiaoxue; Tagiri, Akemi; Kikuchi, Shinichi; Sassa, Hidenori; Komatsuda, Takao

doi:10.3389/fpls.2020.582622

Cited by 3 publications

(2 citation statements)

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“…2015 ). A follow-up study demonstrated lignin reduction at the rachis node in Aegilops tauschii that disarticulate below the rachis node ( Zeng et al. 2020a ).…”

Section: What Are the Physical And Compositional Changes In The Walls...mentioning

confidence: 99%

Grain Disarticulation in Wild Wheat and Barley

Pourkheirandish

Komatsuda

2022

Plant and Cell Physiology

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Our industrial-scale crop monocultures, which are necessary to provide grain for large-scale food and feed production, are highly vulnerable to biotic and abiotic stresses. Crop wild relatives have adapted to harsh environmental conditions over millennia; thus, they are an important source of genetic variation and crop diversification. Despite several examples where significant yield increases have been achieved through the introgression of genomic regions from wild relatives, more detailed understanding of the differences between wild and cultivated species for favorable and unfavorable traits is still required to harness these valuable resources. Recently, as an alternative to the introgression of beneficial alleles from the wild into domesticated species, a radical suggestion is to domesticate wild relatives to generate new crops. A first and critical step for the domestication of cereal wild relatives would be to prevent grain disarticulation from the inflorescence at maturity. Discovering the molecular mechanisms and understanding the network of interactions behind grain retention/disarticulation would enable the implementation of approaches to select for this character in targeted species. Brittle rachis 1 and Brittle rachis 2 are major genes responsible for grain disarticulation in the wild progenitors of wheat and barley that were the target of mutations during domestication. These two genes are only found in the Triticeae tribe and are hypothesized to have evolve by a duplication followed by neo-functionalization. Current knowledge gaps are the molecular mechanisms controlling grain retention in cereals and the genomic consequences of strong selection for this essential character.

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“…2015 ). A follow-up study demonstrated lignin reduction at the rachis node in Aegilops tauschii that disarticulate below the rachis node ( Zeng et al. 2020a ).…”

Section: What Are the Physical And Compositional Changes In The Walls...mentioning

confidence: 99%

Grain Disarticulation in Wild Wheat and Barley

Pourkheirandish

Komatsuda

2022

Plant and Cell Physiology

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“…And two different loci, brittle-rachis 1 (Btr1) and brittle-rachis 2 (Btr2), encoding an α/β-hydrolase superfamily protein and a hypothetical protein, respectively, have been involved in the regulation of brittle rachis (seed shattering) in barley (Pourkheirandish et al, 2015). Homologs of these two genes, Btr1 and Btr2, are also active in two wild wheat relatives, Aegilops longissima and Aegilops tauschii, which also result in seed shattering phenotypes (Zeng et al, 2020a;Zeng et al, 2020b;Zeng et al, 2020c).…”

Section: Differentiation Of the Abscission Zonementioning

confidence: 99%

The abscission regulatory module INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) / HAESA (HAE)-like receptor kinases in Solanaceae species: Functional analysis in Nicotiana benthamiana

Llora¹

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of the abscission process within the abscission zone and organ detachment……….……………...…… 27 1.3.2.4. Differentiation of a protective layer………………………….…. 1.4. The Arabidopsis abscission regulatory module INFLORESCENCE DEFICIENT IN ABSCISSION / HAESA-like receptor kinases………………………… 32 2. Aims and Design of the Present Study………………………………….………………….. 3. Materials and methods…………………………………………………….………….……….. 4. Identification and molecular analysis of INFLORESCENCE DEFICIENT IN ABSCISSION (IDA)-like genes and HAESA (HAE)-like receptor kinases in Solanaceae species of agronomic importance…………………………………...………. 4.1. The IDA-like gene family in the Solanaceae………………………………..……… 48 4.2. Phylogenetic relationship among IDA-like prepropeptides in Solanaceae…….... 4.3. Cis-acting regulatory elements in the promoter regions of the N. benthamiana IDA-like family…………………………..……………… 4.4. The HAE-like gene family in the Solanaceae………………………………..…….. 4.5. Phylogenetic relationship among HAE-like protein kinases in Solanaceae…….. 57 4.6. Amino acid residues involved in the interaction between IDA mature peptides and HAESA-like receptors………………………….…………….. 4.7. Expression patterns of IDA-like and HAE-like genes in Nicotiana benthamiana during growth and abscission……………...…………….……. 4.8. Expression patterns of IDA-like genes in Nicotiana benthamiana during water stress………………………………...…………… 68 4.9. Conclusions………………………………………………………………….………… 70 5. Silencing and overexpression of INFLORESCENCE DEFICIENT IN ABSCISSION and HAESA receptor kinase in flowers of Nicotiana benthamiana: effects on corolla abscission…………………..…......…….……. 5.1. Silencing and overexpression of IDA-like and HAE-like genes using a viral vector based on Citrus leaf blotch virus……………………….……….…. 73 5.2. The inoculation of clbv3'-NbenIDA and clbv3'-NbenHAE constructs arrests corolla abscission………………………...…………………...……… 79 5.3. Anatomy of the corolla tube base in flowers of plants inoculated with the control clbv3' vector and the clbv3'-NbenIDA construct…………..……….…. 5.4. Knockdown of target genes at the base of the corolla tube through inoculation with the clbv3'-NbenIDA and clbv3'-NbenHAE constructs…………….…. 84 5.5. Overexpression of NbenIDA1A decreases plant growth

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Evolution of Wheat Under Cultivation

Feldman,

Levy

2023

Wheat Evolution and Domestication

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The chapter deals with the various steps, periods, and processes that led to the domestication of the wheat as well as with the archaeological sites where domestication took place. Additionally, the chapter describes the ecogeographical characteristics of the area of wheat domestication, the selection of non-brittle rachis, large grain size, rapid and synchronous germination, free-threshing grains, and yield. The genetic basis of non-brittle rachis and free-threshing grains are delt with in details. The formation of hexaploid wheat, T. aestivum, and the spread of its free-threshing form to almost all parts of the globe to become the main cultivated wheat, are reviewed. The production of synthetic Triticum aestivum, and Triticale are also referred to in this chapter.

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The Ectopic Expression of Btr2 in Aegilops tauschii Switches the Disarticulation Layer From Above to Below the Rachis Node

Cited by 3 publications

References 32 publications

Grain Disarticulation in Wild Wheat and Barley

Grain Disarticulation in Wild Wheat and Barley

The abscission regulatory module INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) / HAESA (HAE)-like receptor kinases in Solanaceae species: Functional analysis in Nicotiana benthamiana

Evolution of Wheat Under Cultivation

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