The effect and mechanism of miR-30e-5p targeting SNAI1 to regulate epithelial-mesenchymal transition on pancreatic cancer

Abstract

Accumulating evidence indicates that abnormally expressed microRNAs (miRNAs, miRs) contribute to cancer progression. Nonetheless, the role of miR-30e-5p in pancreatic cancer (PCa) remains unclear. In this study, using quantitative real-time polymerase chain reaction analysis, we found that miR-30e-5p expression was downregulated in human PCa tissues compared with that in normal para-cancerous tissues. After transfecting with miR-30e-5p inhibitors, miR-30e-5p mimics, or empty vectors in the BxPC-3 and PANC-1 cells, respectively, the experiments revealed that the upregulation of miR-30e-5p expression inhibited cell growth, invasion, migration and epithelial-mesenchymal transition (EMT), and promoted apoptosis, while miR-30e-5p downregulation had the opposite effects. RNA sequencing of miR-30e-5p inhibitor-, miR-30e-5p mimic-, and the negative control (NC)-treated groups revealed that miR-30e-5p may affect epithelial cell differentiation, cell growth and death. Next, the snail family transcriptional repressor 1 (SNAI1) was predicted and verified as the target gene of miR-30e-5p using bioinformatics analysis and luciferase assays. SNAI1 expression levels were decreased in the PCa cells transfected with miR-30e-5p mimics, whereas the opposite was observed in the cells transfected with miR-30e-5p inhibitors. Subsequently, PCa cells were transfected with a vector overexpressing SNAI1 (OE-SNAI1) and miR-30e-5p mimics, miR-30e-5p inhibitors, or empty vectors. Compared with that in the OE-SNAI1 + miR-30e-5p NC group, transfection with OE-SNAI1 + miR-30e-5p mimics inhibited the PCa cell growth, migration, and increased apoptosis, whereas transfection with OE-SNAI1 + miR-30e-5p inhibitors had the opposite effects. In conclusion, miR-30e-5p potentially inhibits PCa cell proliferation, migration, and invasion via the SNAI1/EMT axis.

Identification and validation of a prognostic anoikis-related gene signature in papillary thyroid carcinoma by integrated analysis of single-cell and bulk RNA-sequencing

“…[32] Besides, SNAI1 have a positive effect on the development and invasion of various tumors through inducing EMT. [33][34][35] In addition, IRX1, a member of the iroquois homeobox transcription factor family, has been identified as a tumor suppressor in several cancers, including lung adenocarcinoma, gastric cancer and invasive ductal carcinoma. [36][37][38][39] In our study, we found that high expressions of CDKN2A, SCD and SNAI1 were closely related to the poor prognosis, which was consistent with previous studies.…”

Prediction of Early- and Late-Onset Pre-Eclampsia in the Preclinical Stage via Placenta-Specific Extracellular miRNA Profiling

“…miR-22 triggers EMT by inhibiting the translation of SFRP2 and PCDH15, which repress the Wnt/b-catenin pathway, essential for cell growth, invasion, and stemness [52]. miR-30e-5p inhibits cell proliferation, migration, and invasion via the targeting of snail family transcriptional repressor 1 (SNAI1), whereby the up-regulation of which is the main characteristic of EMT [53]. Additionally, it was found that the expression levels of let-7d-5p and miR-20a-5p were increased [54,55], while the expression level of let-7i-5p was decreased [56] in placental tissues or blood plasma samples collected from patients with PE.…”

MicroRNAs as the critical regulators of epithelial mesenchymal transition in pancreatic tumor cells

“…MiR-30e-5p had a tumor suppressor function in regulation of PC cell proliferation, EMT, and migration through SNAI1 suppression. There was also miR-30e-5p down regulation in PC tissues in comparison with normal samples [ 33 ]. MiR-199 inhibited self-renewal of gemcitabine (GEM)-treated PC cells.…”