The Effect of 1%, 2% and 4% Sucrose Induction on Zebrafish Cardiomyocyte Damage

Abstract

One of the complications of diabetes is cardiovascular disease. Some studies mention insulin resistance in the cadiac muscle will increase the occurrence of inflammation and necrosis of cardiac muscle cells. The study aims to prove the effect of sucrose induction on zebrafish cardiomyocyte damage. The research was conducted in the laboratory, using a post test group control design. The research sample used zebra fish. The research group consisted of normal control group (N) and sucrose induction group 1% (G1), 2% (G2) and 4% (G3). Hyperglycemia induction is a modification of previous researchers. The process of inducing 1%, 2%, 4% sucrose in zebrafish bath water for 28 days which is replaced every 2 days. The treatment process followed laboratory procedures. At the end of the study, zebrafish were fed for 10 hours, then anesthetized by placing the fish in ice water for 10 minutes. Furthermore, blood glucose levels were measured through the tail using glucotest. Normal glucose levels of zebrafish are between 40-90 mg/dL. Cardiac organ was taken and histology preparate was made. Cardiac damage in the form of leukocyte infiltration and the area of cardiac muscle cell edema was calculated histologically, with HE staining, 400 x magnification, using image J software. Measurement data were analyzed using ONEWAY ANOVA, with a significance level of p<0.05. The average percentage of leukocyte cell infiltration of G3 was 40% higher than N (15%), G1 (19%) and G2 (27%) with p<0.05. G3 odem area of (42%) was greater than the normal group N (5%), G1 (18%) and G2 (34%) with p<0.05. It is suspected that this condition is due to the effect of hyperglycemia due to the induction of 4% sucrose (179mg/dL). From this study it can be concluded that 4% glucose induction causes damage to zebrafish heart cells which is characterized by the presence of leukocyte cell inflitration of 40% and odem area of 42%.