The Effect of 1,2-Indanedione, a Latent Fingerprint Reagent on Subsequent DNA Profiling

Abstract

The compound 1,2-indanedione was recently introduced in our laboratory as an operational reagent for developing latent fingerprints on porous surfaces. As part of the reagent implementation, a study was carried out in order to determine whether either of the two operational 1,2-indanediones formulations interferes with further DNA profiling. Both formulations are based on HFE7100 solvent. One is acidic and the other neutral. In a controlled experiment, known donors attached stamps to envelopes by licking them. The stamped envelopes were initially treated with either one indanedione formulation or the other, and DNA was then extracted for STR typing. No differences were observed between the STR profiles obtained from treated and untreated stamps and envelopes, indicating that 1,2-indanedione does not adversely affect the extraction and subsequent amplification of the STRs examined. However, preliminary results indicate that potential DNA analysis depends on the time interval between the indanedione treatment and DNA extraction as no DNA can be recovered six days following treatment. For this reason, it is strongly recommended to extract DNA from treated items of evidence as soon as possible after indanedione treatment.

DNA recovery after sequential processing of latent fingerprints on copy paper

“…Latent fingerprints on copy paper treated with single‐reagent and sequential fingerprint development processes produced DNA profiles with varying degrees of success. The single‐reagent treatments that were the least harmful to downstream DNA analysis were IND‐Zn and IND‐Zn/laser, a conclusion that has been demonstrated previously [33]. For the sequential treatments containing IND‐Zn and IND‐Zn/laser, the DNA yield and overall quality of the STR profiles generally decreased with the addition of each sequential treatment (e.g., ninhydrin, ORO, PD).…”

1,2-Indanedione (IND) Reagent for Detection of Latent Fingermarks: A Review

“…Azoury et al, [76] reported that IND did not affect the isolation and amplification of STRs examined and therefore, DNA could be successfully typed from such processed items. No variations were observed in the STR profiles generated from processed and unprocessed envelopes and stamps.…”

Forensic Science

“…A sensitive method for the quantitation of genomic DNA by Alu amplification is presented (27). The effect of 1,2-indanedione, a latent fingerprint detection reagent, on subsequent DNA profiling is described (28). The theory and application of the transfer of trace quantities of DNA through skin contact is reviewed (29).…”

“…Excellent performance of 1,2-indanedione as a latent fingerprint detection reagent has been reported (216). The effect of 1,2-indanedione, a latent fingerprint reagent, on subsequent DNA profiling has been studied (217). Chemical processes involved in the development of latent fingerprints using the cyanoacrylate fuming method have been studied (218).…”