The EFFECT OF 17β-Estradiol ON THE GONOPODIAL DEVELOPMENT AND SEXUAL ACTIVITY OF GAMBUSIA HOLBROOKI

Doyle, C. J.; Lim, Richard P.

doi:10.1897/1551-5028(2002)021<2719:teoeot>2.0.co;2

Cited by 33 publications

(41 citation statements)

References 35 publications

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“…Poeciliids have been widely used to investigate the in vivo effects of reproductively active endocrine disrupting compounds (e.g., Doyle and Lim, 2002, Angus et al, 2005, Rawson et al, 2008. They are less commonly used to examine the effects and exposure to POPs.…”

Section: Use Of Mosquitofish In Determination Of In Vivo and In Situ mentioning

confidence: 99%

Bioactivity of POPs and their effects in mosquitofish in Sydney Olympic Park, Australia

Rawson

Tremblay

Warne

et al. 2009

Science of The Total Environment

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a b s t r a c tThe site of the 2000 Olympic Games (Sydney Olympic Park (SOP), Sydney, Australia) was contaminated by persistent organic pollutants (POPs) prior to remediation in the 1990s. This study investigates the bioactivity of POPs in the sediment and water of wetlands across SOP by in vitro 2,3,7,8-TCDD equivalence (TCDDeq) measurement (H4IIE cell line bioassay). Further, it examines whether disturbance of these sediments is likely to mobilise ligands for this receptor into the water column. Exposure to aryl hydrocarbon receptor (AhR) ligands was measured in vivo using hepatic cytochrome P4501A (CYP1A) induction (EROD) in the mosquitofish (Gambusia holbrooki). Aqueous TCDDeq ranged from 0.013 to 0.057 pM in SOP wetlands which was significantly (p b 0.05) less that in urban reference sites. These concentrations were not correlated to physical or chemical characteristics of the wetlands. In the sediments, TCDDeq ranged from 0.0016 to 7.06 µg/kg and these were not significantly (p ≥ 0.05) different to that measured in urban reference sites. Simulated disturbance of small quantities of sediment in water samples significantly (p b 0.05) increased the levels of TCDDeq measured in the water. Sediment TCDDeq was correlated to sediment ΣPAH concentration in 2006 and sediment ΣPCB, ΣDDT concentrations and fine sediment grain size in 2005. While fish at one SOP wetland had hepatic EROD activity elevated above the estimated basal level for this species, these were at the lower end of the range measured in urban impacted, non-remediated wetlands. EROD activity was positively correlated with both the sediment ΣPCB load and aqueous TCDDeq. Increased catchment size was correlated with increased EROD activity suggesting an even spread of POPs throughout the residential areas of the Sydney metropolitan area. The concentration of bioactive POPs in the wetlands of SOP is therefore low relative to urban reference sites demonstrating the ongoing success of the remediation program..

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Section: Use Of Mosquitofish In Determination Of In Vivo and In Situ mentioning

confidence: 99%

Bioactivity of POPs and their effects in mosquitofish in Sydney Olympic Park, Australia

Rawson

Tremblay

Warne

et al. 2009

Science of The Total Environment

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“…This is because as Sumpter and Johnson (2005) recorded 'one animal's poison may not be another's'. However, G. holbrooki has been widely used in this role (e.g., Angus et al 2005;Batty and Lim 1999;Doyle and Lim 2002).…”

Section: Resultsmentioning

confidence: 99%

“…They were then transported to the laboratory where they were euthanized by immersion in 400 mg/L benzocaine (cf. Doyle and Lim 2002;Rawson et al 2008). …”

Section: Australian Zoologist Volume 35 (4)mentioning

confidence: 99%

“…After male fish had been euthanized they were viewed under a dissecting microscope at 40X magnification to determine if the terminal hooks on the gonopodium had developed, reported by Doyle and Lim (2002) to be evidence of maturity. Each mature male was then weighed, and again using 40X magnification, and a 50 mm glass slide rule with 0.1 mm divisions, the gonopodium rays 4 and 6 (ray 4 is under androgenic control, ray 6 is not, see Figure 1) were measured as the basis of establishing if endocrine disruption was occurring (cf.…”

Section: Australian Zoologist Volume 35 (4)mentioning

confidence: 99%

“…An alternative approach to assessing endocrine disruption in aquatic systems is to use an indicator species. Mosquito fish Gambusia holbrooki, considered to be a useful sentinel species (Pyke 2005), has been shown to respond to estrogens in laboratory studies of 17β-Estradiol (Doyle and Lim 2002) and 17β-Ethynylestradiol (Angus et al 2005). They have also been observed to be an indicator of endocrine disruption in aquatic systems (Batty and Lim 1999).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Answering questions on the impact of recycled water on wildlife usingGambusia holbrookias a surrogate

Norris

Burgin

2011

Australian Zoologist

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The disruption of endocrine systems due to environmental contaminants potentially impacts on developmental, behavioural, regulatory, and reproductive systems of wildlife. A major source of exposure of wildlife (terrestrial and aquatic) to endocrine disrupting compounds is through contact with contaminated surface waters. Current testing routines in aquatic systems have not been designed specifically to assess endocrine disruption properties or, alternatively, methods tend not to be fully developed. An alternative approach is to use a response sensitive to these chemicals such as gonopodium length in the pest species Gambusia holbrooki as an indicator. This species was used as a surrogate for native aquatic species to assess endocrine disruption in wetlands that are used for the storage of stormwater or treated sewage effluent. These were compared with adjacent wetlands used for watering stock (farm dams) that were not contaminated with these pollutants. Deformities in the mosquito fish were found that were consistent with endocrine disruption caused by sex steroids and/ or their mimics in the first in the sequence of each pollution type but not in farm dams. We concluded that the first wetland in the sequence of recycling stormwater or treated sewage effluent could cause detrimental effects to wildlife but not in subsequent wetlands in a sequence of water storage systems. Since all waters are moved through at least three wetlands before being used for irrigation, or released into the river system, the impact on wildlife is likely to be minimal beyond the first wetlands.

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Intersex and liver alterations induced by long‐term sublethal exposure to 17α‐ethinylestradiol in adult male Cnesterodon decemmaculatus (Pisces: Poeciliidae)

Young

López

Cristos

et al. 2016

Enviro Toxic and Chemistry

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The aim of the present study was to assess the responses of the gonopodium morphology and the gonadal and liver histology of adult male Cnesterodon decemmaculatus to sublethal long-term exposure concentrations of 17α-ethinylestradiol (EE2). Two experiments were conducted exposing the fish to waterborne concentrations of EE2 ranging from 20 ng/L to 200 ng/L for 8 wk, 12 wk, and 16 wk. Intersex gonads were observed after 8 wk and 16 wk in fish exposed to 200 ng EE2/L and 100 ng EE2/L, respectively. Oocytes' development from testis germ cells and replacement of the efferent duct periodic acid-Schiff-positive secretion surrounding spermatozeugmata by parenchymal tissue and duct structure alterations were the major observed changes in the gonads. In contrast, no response was observed in the gonopodium morphology. Liver histology was also altered, showing increasing steatosis, single-cell necrosis to generalized necrosis, and disruption of acinar organization from 100 ng EE2/L to 200 ng EE2/L. In summary, the present results showed that although EE2 was not able to alter the morphology of a developed gonopodium, it was capable of inducing development of testicular oocytes in adult male C. decemmaculatus at environmentally relevant concentrations. Thus, externally normal but intersex C. decemmaculatus males would be expected in the wastewater-receiving streams that the species inhabits. According to the literature, the present study would be the first indicating estrogen-induced intersex in adult male poeciliid. Environ Toxicol Chem 2017;36:1738-1745. © 2016 SETAC.

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The EFFECT OF 17β-Estradiol ON THE GONOPODIAL DEVELOPMENT AND SEXUAL ACTIVITY OF GAMBUSIA HOLBROOKI

Cited by 33 publications

References 35 publications

Bioactivity of POPs and their effects in mosquitofish in Sydney Olympic Park, Australia

Bioactivity of POPs and their effects in mosquitofish in Sydney Olympic Park, Australia

Answering questions on the impact of recycled water on wildlife usingGambusia holbrookias a surrogate

Intersex and liver alterations induced by long‐term sublethal exposure to 17α‐ethinylestradiol in adult male Cnesterodon decemmaculatus (Pisces: Poeciliidae)

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