The Effect of 2,3,4′,5-Tetrahydroxystilbene-2-O-β-D-Glucoside on Pressure Overload-Induced Cardiac Remodeling in Rats and Its Possible Mechanism

Abstract

The aim of the present study was to investigate the effects of 2,3,4',5-tetrahydroxystilbene-2-O-beta-D-glucoside, an active component extracted from Polygonum multiflorum, on pressure overload-induced cardiac remodeling in rats. A rat model with cardiac remodeling was induced by abdominal aortic banding. 2,3,4',5-Tetrahydroxystilbene-2-O-beta-D-glucoside (30, 60, 120 mg/kg/day) was administered 3 days after abdominal aortic banding and continued for 30 days. The abdominal aortic banding-treated rats had significantly elevated blood pressure, left ventricular hypertrophy, and myocardial fibrosis. Left ventricular hypertrophy was characterized by an increase in the ratios of the heart and left ventricular weights to body weight, and increased myocyte cross-sectional areas, hypertrophic ventricular septum, and left ventricular posterior wall. The accumulation of myocardial interstitial perivascular collagen and elevated cardiac hydroxyproline content indicated myocardial fibrosis. The pathological changes above were attenuated by 2,3,4',5-tetrahydroxystilbene-2-O-beta-D-glucoside. Additionally, it markedly reduced collagen I and III expressions and regulated matrix metalloproteinase-2,9 and inhibitors of metalloproteinase expressions, as markers of myocardial fibrosis. Furthermore, we explored the underlying mechanisms for such effects of 2,3,4',5-tetrahydroxystilbene-2-O-beta-D-glucoside. The results showed that it significantly reduced myocardium angiotensin II, enhanced the activities of superoxide dismutase and glutathione peroxidase in serum and myocardial tissue, as well as inhibited protein expression of transforming growth factor-β1 and phosphorylation of extracellular signal-regulated kinase 1/2 and p38 mitogen-activated protein kinase in the myocardial tissue. Our results suggest that 2,3,4',5-tetrahydroxystilbene-2-O-beta-D-glucoside could prevent cardiac remodeling induced by pressure overload in rats. The underlying mechanisms may be related to a decreasing angiotensin II level, an antioxidant effect of the tested compound, suppression of transforming growth factor-β1 expression, and inhibition of extracellular signal-regulated kinase 1/2 and p38 mitogen-activated protein kinase activation.

Tetrahydroxystilbene Glycoside Improves Microvascular Endothelial Dysfunction and Ameliorates Obesity-Associated Hypertension in Obese ZDF Rats Via Inhibition of Endothelial Autophagy

“…These data suggest that TSG had a promising anti-aging effect by regulation of Klotho expression [19]. TSG is a free radical-scavenger [22], and exerts anti-aging effects in mammals via a neural insulin/IGF-1 signaling pathway [19]. TSG exhibits strong anti-oxidative and anti-inflammatory effects, as well as protecting mammals from cerebral ischemia and/or reperfusion injury [23, 43].…”

“…2, 3,5, 4’-tetrahydroxystilbene-2-O- β -D-glycoside (TSG) is the main bioactive ingredient extracted from the traditional Chinese medicinal herb Polygonum multiflorum Thunb which has been used safely in China for approximately two millennia, and has been reported to have a wide spectrum of pharmacologic functions. TSG has been reported to help people live longer [18], protect impaired neural networks [19], improve blood flow [20], attenuate inflammatory responses [21], prevent the production of oxygen radicals and protect against oxidized LDL-induced endothelial dysfunction [22]. Previously, we demonstrated that TSG inhibits lysophosphatidylcholine (LPC)-induced apoptosis of human umbilical vein endothelial cells (HUVECs) by activating glutathione peroxidase and superoxide dismutase, clearing intracellular reactive oxygen species (ROS), and reducing lipid peroxidation [21, 23].…”

2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside protects murine hearts against ischemia/reperfusion injury by activating Notch1/Hes1 signaling and attenuating endoplasmic reticulum stress

“…C57Bl/6 mice were orally gavaged with TSG at a dose of 60 mg/kg body weight per day for 2 weeks. The TSG dose was selected based on previous studies [16,27,28] . Two weeks after the treatment, MI/R surgery was performed as previously described [29] .…”

Potential Application of Tetrahydroxystilbene Glucoside in Treatment of Alzheimer’s Disease

“…Recent progresses suggested that TSG might have much pharmacological properties including antioxidant, anti-aging, antiinflammation, anti-atherosclerosis, anti-hyperlipidaemia, anti-platelet activity, ameliorate diabetic nephropathy, neuroprotection, hair growth, and cardioprotective effects [47][48][49][50][51][52][53][54][55][56][57][58] . There are increasing reports about the roles of TSG on learning and memory changes of animal AD models, as well as possible action mechanisms.…”