The effect of 2,4‐dinitrophenol and related compounds on bile secretion

Abstract

SUMMARY1. 2,4-dinitrophenol, 2,4-dinitrophenetole, 2,4-dinitronaphthol, 4,6-dinitro-o-cresol, and to a lesser extent picric acid, produced an increase in bile flow and a rise in body temperature in the anaesthetized dog. The total biliary bromsulphalein (BSP) excretion in unit time was either slightly reduced, increased or remained at its pre-injection level.2. Picramic acid, the nitrochlorophenols and 2,4-dinitrobenzaldehyde caused a moderate increase in bile flow without an effect on the temperature of the animal.3. The three mononitrophenols, the five remaining isomeric dinitrophenols, isopicramic acid, the aminonitrophenols, phenol, 2,4-dinitroanisole, 2,4-dinitrobenzoic acid, 2,4-dinitrobenzene sulphonic acid, 2,4-dinitroresorcinol and 4-nitracatechol had little effect on bile secretion or body temperature.4. It thus appears that, in order for a compound of this type to have a pronounced effect on bile secretion, it is necessary to have nitro groups in positions 2 and 4 of the benzene ring, and a free or potential hydroxyl group.

Reproductive and developmental toxicity screening study of 2,4‐dinitrophenol in rats

“…Consideration of these findings suggests that adverse effects on the survival and growth of offspring are due to a combination of direct effects of DNP and/or its metabolites and altered maternal physiology. DNP produced dose-related hyperthermia resulted from the uncoupling of oxidative phosphorylation action (Tainter and Cutting, 1933;Pugh and Stone, 1968;ATSDR, 1995). Hyperthermia is known to be teratogenic and embryolethal in rats (Cockroft and New, 1978;Germain et al, 1985), and rectal temperature at 41.08C, an elevation of 2.58C, for 1 h was the threshold combination for teratogenic potential (Germain et al, 1985).…”

The effect of sodium salicylate on bile secretion in the dog.

“…A number of substances other than bile salts and secretin have been shown to increase the flow of hepatic bile in the dog. These include 2,4-dinitrophenol, and structurally related compounds (Pugh & Stone, 1968). Sodium salicylate has also been shown to act as a choleretic in a variety of species including the dog (Okada, 1915).…”

“…These include 2,4-dinitrophenol, and structurally related compounds (Pugh & Stone, 1968). Sodium salicylate has also been shown to act as a choleretic in a with sodium pentobarbitone (30 mg/kg i.v.).…”