The Effect of 2-Thioxo Imidazolidin-4-Ones (2- Thiohydantion) on Anticancer Activity: An in Vitro Study

Abstract

Background and Purpose: Cancer is one of the most common factors of death in society today. Despite much research into cancer and its treatment, it is still one of the greatest health problems. The biggest obstacle to cancer treatment is that they are inherently resistant to chemotherapy or become drug resistant during treatment. For this reason, researchers around the world have made great efforts to identify new natural or synthetic compounds with anticancer properties. So the aim of this study is to find out the effect of 2-Thioxo imidazolidin-4-ones (2-Thiohydantion) on anticancer activity. Materials and Methods:Imidazoles are compounds that have antibacterial, antifungal and antitumor properties in many studies. Through the results of this study, we discovered effectiveness of the new compound of 2-Thioxo imidazolidin-4-ones (2 Thiohydantion) as an anticancer agent against a cell line that is a cancer model system. To determine the IC 50 of this compound, we treated the cancer cells by different concentrations (10, 20, 30, 40, 80 and 160 μg / ml) and then the cells were evaluated by MTT assay after 24 and 48 hour intervals.Results: After preparing the required compound, MTT assay showed that the IC 50 of 2-Thioxo imidazolidin-4-ones (2-Thiohydantion) compound on MCF7 cells, 135 and 40 μg / ml, respectively, after 24 and 48 h, respectively. In addition, the toxicity of these compounds on normal cells was evaluated, which is very different from their toxicity for cancer cells, and this is a very important feature. 2-Thioxo imidazolidin-4ones (2-Thiohydantion) has the ability to stimulate cell death in cancer cells, and these results provide new insights into the use of these compounds in cancer treatment. Conclusion:So 2-Thioxo imidazolidin-4-ones (2-Thiohydantion) at different concentrations significantly reduced the growth of cancer cells compared to the control group (50% inhibition of cell growth). Future studies will need to investigate the mechanism of this cytotoxicity as well as the possible anticancer effectiveness of this compound in vitro and in vivo.

Evaluation of 2-Thioxoimadazolidin-4-one Derivatives as Potent Anti-Cancer Agents through Apoptosis Induction and Antioxidant Activation: In Vitro and In Vivo Approaches

“…New 2-thioxo-imidazolidin-4-one derivatives contain pyrimidine, diaryl, triaryl, cycloalkyl amines, and glucopyranosyl substituents and their aryl and halogen and alkyl derivatives, and other substituents with varied monocyclic (thiadiazole, sulfadiazole) heterocycles linked directly or through alkyl chain with the imidazolidine nucleus [ 20 ]. Several published articles and patents discussed the discovery and development of various promising 2-thioxo-imidazolidin-4-one derivatives in cancer therapy [ 21 ].…”