The effect of 4-(1-naphthylvinyl)-pyridine on the acetylcholine system and on the number of synaptic vesicles in the central nervous system of the rat

Kása, P.; Szepesy, G.; Gulya, Károly; Bánsághy, K.; Rakonczay, Zoltán

doi:10.1016/0197-0186(82)90014-6

Cited by 10 publications

(1 citation statement)

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“…For measurement of the free choline (Ch) content, lo-$ samples were incubated in the incubation mixture whereas for assay of ACh content, 5 p1 AChE (500 unitsiml) was added to a 75-15.1 aliquot of the samples (to hydrolyze ACh) and the mixture was then incubated for IS min at 37°C. The AChE was then inactivated at 100°C for 60 s. The amounts of ACh and Ch in the samples were determined by the method of Eckernas and Aquilonius (1967) and as described by Kasa et al (1982). The incubation mixture contained (in final concentration) 50 mM sodium phosphate buffer (pH 8.0), 100 mM NaCl, 0.5 mM EDTA, 0.2 mM eserine sulfate, 70 p M [14C]acetyl-coenzyme A, and 10 p1 purified ChAT (specific activity: 0.38 pmol min-'mg-I protein).…”

Section: Assaysmentioning

confidence: 99%

Modulation of the Acetylcholine System in the Superior Cervical Ganglion of Rat: Effects of GABA and Hypoglossal Nerve Implantation After In Vivo GABA Treatment

Kása

Dames

Rakonczay

et al. 1985

Journal of Neurochemistry

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gamma-Aminobutyric acid (GABA) was applied to the superior cervical ganglion (SCG) of CFY rats in vitro and in vivo, with or without implantation of a hypoglossal nerve, to evaluate the effects of these experimental interventions on the acetylcholine (ACh) system, which mainly serves the synaptic transmission of the preganglionic input. Long-lasting GABA microinfusion into the SCG in vivo apparently resulted in a "functional denervation." This treatment reduced the acetylcholinesterase (AChE; EC 3.1.1.7) activity by 30% (p less than 0.01) and transiently increased the number of nicotinic acetylcholine receptors, but had no significant effect on the choline acetyltransferase (acetyl-coenzyme A:choline-O-acetyltransferase; EC 2.3.1.6) activity, the ACh level, or the number of muscarinic acetylcholine receptors. The relative amounts of the different molecular forms of AChE did not change under these conditions. In vivo GABA application to the SCG with a hypoglossal nerve implanted in the presence of intact preganglionic afferent synapses exerted a significant modulatory effect on the AChE activity and its molecular forms. The "hyperinnervation" of the ganglia led to increases in the AChE activity (to 142.5%, p less than 0.01) and the 16S molecular form (to 200%, p less than 0.01). It is concluded that in vivo GABA microinfusion and GABA treatment in the presence of additional cholinergic synapses has a modulatory effect on the elements of the ACh system in the SCG of CFY rats.

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Section: Assaysmentioning

confidence: 99%