The effect of a cardiopulmonary bypass system with biocompatible coating on fibrinogen levels determined by the TEG – functional fibrinogen method: preliminary results

Fluger, Ivo; Maderová, Kateřina; Šimek, Martin; Zapletalová, Jana; Lonský, Vladimír

doi:10.1177/0267659111415891

Cited by 8 publications

(4 citation statements)

References 11 publications

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“…TEG has been used to study in vitro effects of fibrinogen on coagulation of plasma deficient in coagulation factors and diluted by colloids [77, 78], and the effect of a cardiopulmonary bypass system with biocompatible coating on fibrinogen levels [38]. ROTEM has been used to determine the usefulness of fibrinogen substitution to reverse dilutional coagulopathy in in vitro and in vivo models [79–81].…”

Section: Measurement Of Fibrinogen Levels and Functionsmentioning

confidence: 99%

Thromboelastography and Thromboelastometry in Assessment of Fibrinogen Deficiency and Prediction for Transfusion Requirement: A Descriptive Review

Peng

Nascimento

Beckett

2018

BioMed Research International

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Fibrinogen is crucial for the formation of blood clot and clinical outcomes in major bleeding. Both Thromboelastography (TEG) and Rotational Thromboelastometry (ROTEM) have been increasingly used to diagnose fibrinogen deficiency and guide fibrinogen transfusion in trauma and surgical bleeding patients. We conducted a comprehensive and comparative review on the technologies and clinical applications of two typical functional fibrinogen assays using TEG (FF TEG) and ROTEM (FIBTEM) for assessment of fibrinogen level and deficiency, and prediction of transfusion requirement. Clot strength and firmness of FF TEG and ROTEM FIBTEM were the most used parameters, and their associations with fibrinogen levels as measured by Clauss method ranged from 0 to 0.9 for FF TEG and 0.27 to 0.94 for FIBTEM. A comparison of the interchangeability and clinical performance of the functional fibrinogen assays using the two systems showed that the results were correlated, but are not interchangeable between the two systems. It appears that ROTEM FIBTEM showed better associations with the Clauss method and more clinical use for monitoring fibrinogen deficiency and predicting transfusion requirements including fibrinogen replacement than FF TEG. TEG and ROTEM functional fibrinogen tests play important roles in the diagnosis of fibrinogen-related coagulopathy and guidance of transfusion requirements. Despite the fact that high-quality evidence is still needed, the two systems are likely to remain popular for the hemostatic management of bleeding patients.

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Section: Measurement Of Fibrinogen Levels and Functionsmentioning

confidence: 99%

Thromboelastography and Thromboelastometry in Assessment of Fibrinogen Deficiency and Prediction for Transfusion Requirement: A Descriptive Review

Peng

Nascimento

Beckett

2018

BioMed Research International

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“…There are numerous studies on FB adsorption or deposition on various surfaces to improve biocompatibility and anti-thrombogenicity. 27–30…”

Section: Introductionmentioning

confidence: 99%

Plasma-activated fibrinogen coatings onto poly(vinylidene fluoride) surface for improving biocompatibility with tissues

Stoleru¹,

Baican

Coroabă³

et al. 2015

Journal of Bioactive and Compatible Polymers

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CO 2 , N 2 , and N 2 /H 2 radiofrequency plasma exposure was used for functionalization of poly(vinylidene fluoride) surface aiming the fibrinogen immobilization. Fibrinogen was immobilized onto poly(vinylidene fluoride) surface using both simple plasma activation and covalent coupling. The modified surfaces have been characterized by X-ray photoelectron spectroscopy, attenuated total reflectance-Fourier transform infrared spectroscopy, near infrared-chemical imaging, atomic force microscopy, and wettability measurements, and the obtained materials were tested as supports for fibroblast cell cultures. The plasma type and the immobilization procedure have influenced the fibrinogen attachment onto the poly(vinylidene fluoride) surface, which was achieved mainly through amide bonds when using coupling agents. Covalent immobilization of fibrinogen onto poly(vinylidene fluoride) surface leads to a more stable protein-modified polymer surface. Non-cytotoxic plasma-based coating technology has the ability to covalently immobilize bioactive molecules for surface modification of some biomaterials that mainly could be achieved by the immobilization of proteins such as fibrinogen that triggers desirable cellular responses. The fibrinogen-modified poly(vinylidene fluoride) materials showed increased cell viability of fibroblasts. Cell viability was enhanced by plasma-activated fibrinogen coatings onto poly(vinylidene fluoride) surface, this being more significant if coating was linked further by a coupling reaction. Hence, they could be good candidates for biomedical applications.

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“…Four major assays have been proposed in people for assessment of functional fibrinogen by viscoelastic coagulometers. Options for TEG include glycoprotein IIb/IIIa (GPIIb/IIIa) inhibition via abciximab, inhibition of platelet actin polymerization via cytochalasin D, the combination of abciximab and cytochalasin D, or the use of Activator F in heparinized whole blood . The ROTEM system utilizes citrated whole blood with the commercially available Fib‐TEM reagent (cytochalasin D) with Ex‐TEM (exogenous tissue factor) as an activator.…”

Section: Evidence Summarymentioning

confidence: 99%

“…Abciximab dose‐dependently inhibits platelet function in people and dogs . In people, abciximab results in inhibition of the platelet component to TEG, however despite clear evidence of decreased platelet function, the canine TEG is not affected by abciximab .…”

Section: Evidence Summarymentioning

confidence: 99%

Systematic evaluation of evidence on veterinary viscoelastic testing Part 5: Nonstandard assays

Brainard

Goggs

Méndez-Angulo

et al. 2014

J Vet Emergen Crit Care

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Nontraditional assays identified included thrombelastography (TEG)-PlateletMapping (PM), functional fibrinogen assessment, and rapid-TEG (r-TEG). Direct veterinary evidence was found for only the ADP-activated PM, which appears to generate valid data in dogs but not cats or horses. Arachidonic acid activated PM shows high variability and requires further assessment and validation in veterinary species. Functional fibrinogen assays may be performed in veterinary species but may require modification due to species differences in response to abciximab. While tissue factor (TF)-activated TEG has been well described in the veterinary literature, the specific r-TEG assay has not been assessed, but presumably would be effective for generating TEG tracings and values for maximum amplitude and angle in shorter periods of time than some traditional assays.

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The effect of a cardiopulmonary bypass system with biocompatible coating on fibrinogen levels determined by the TEG – functional fibrinogen method: preliminary results

Cited by 8 publications

References 11 publications

Thromboelastography and Thromboelastometry in Assessment of Fibrinogen Deficiency and Prediction for Transfusion Requirement: A Descriptive Review

Thromboelastography and Thromboelastometry in Assessment of Fibrinogen Deficiency and Prediction for Transfusion Requirement: A Descriptive Review

Plasma-activated fibrinogen coatings onto poly(vinylidene fluoride) surface for improving biocompatibility with tissues

Systematic evaluation of evidence on veterinary viscoelastic testing Part 5: Nonstandard assays

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