The effect of a glucose load on plasma fatty acids and lipolysis in adipose tissue of obese diabetic and non-diabetic patients

Gilbert, Christine; Kaye, Josse; Galton, D. J.

doi:10.1007/bf01219669

Cited by 27 publications

(7 citation statements)

References 7 publications

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“…The basal rate of lipolysis was about twice as rapid in adipose tissue of the untreated diabetics as in the control group (Figs. 1 and 2), which confirms earlier observations made on juvenile [7,12,32], maturityonset diabetics [8] and obese diabetics [18]. Differences in the basal concentration of tissue cAMP are not a likely explanation of the findings relating to basal lipolysis.…”

Section: Basal Lipolysissupporting

confidence: 87%

“…The accelerated lipid mobilisation from the fat depots in human diabetes mellitus is well established by measurements of free fatty acids (FFA) and glycerol in plasma (see 13 for references) as well as by studies on subcutaneous adipose tissue in vitro [7,8,12,18,32]. Insulin deficiency and/or increased action by adipokinetic hormone are generally considered to be the pathophysiological background.…”

Section: Methodsmentioning

confidence: 99%

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Abnormalities in the adrenergic control and the rate of lipolysis in isolated human subcutaneous adipose tissue in diabetes mellitus

Arner¹,

Östman²

1976

Diabetologia

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Subcutaneous adipose tissue was obtained from 9 patients with untreated diabetes mellitus and from 13 obese nondiabetics. After incubation with isoprenaline or noradrenaline, glycerol release and tissue cyclic AMP (cAMP) were determined. Basal glycerol release was twice as rapid from the diabetic adipose tissue. With isoprenaline, the cAMP concentration and the glycerol production was significantly higher in the diabetic adipose tissue. Noradrenaline did not increase glycerol production or cAMP concentration in the diabetic adipose tissue. Subcutaneous adipose tissue was also removed from the diabetics after antidiabetic treatment. Basal lipolysis was significantly reduced and noradrenaline significantly increased both glycerol release and cAMP production. With isoprenaline, cAMP production and glycerol release were significantly less after antidiabetic treatment than in the untreated state. The data provide evidence for increased a-as well as fl-adrenergic receptor sensitivity in human subcutaneous adipose tissue of untreated diabetic patients.

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Section: Basal Lipolysissupporting

confidence: 87%

Section: Methodsmentioning

confidence: 99%

Abnormalities in the adrenergic control and the rate of lipolysis in isolated human subcutaneous adipose tissue in diabetes mellitus

Arner¹,

Östman²

1976

Diabetologia

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“…In the present study a rather large amount of glucose was given together with the lipid infusion. The effects of glucose on FFA metabolism include increased tissue re-esterification [34] and increased plasma fractional catabolic rate [35,36]. These effects explain why plasma FFA levels were lower here, and make it difficult to evaluate a possible relation between FFA and LPL release.…”

Section: Discussionmentioning

confidence: 91%

Effect of medium‐ and long‐chain triglyceride infusion on lipoprotein and hepatic lipase in healthy subjects

Nordenström

Neeser

Olivecrona

et al. 1991

Eur J Clin Investigation

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Plasma lipolytic activity and hydrolysis of intravenous fat were studied in six healthy subjects during infusion of a long-chain triglyceride (LCT) fat emulsion (Intralipid 20%) or of a medium-chain triglyceride (MCT)/LCT emulsion (Lipofundin MCT 20%). The fat emulsions were infused continuously at a rate of 0.17 g triglyceride kg-1 body weight (BW)h-1 for 6 h in random order at 7-day intervals. A continuous infusion of glucose (0.18 g kg-1 BW h-1) was administered for a period of 7 h and was started 1 h before the lipid infusion. Infusions of both types of fat increased plasma triglyceride (TG), free fatty acid (FFA) and lipoprotein lipase (LPL) levels and steady-state values were present during the 3rd to 5th h of infusion. MCT/LCT infusion resulted in higher plasma levels at steady-state of TG (3.63 +/- 0.45 [SEM] vs 2.73 +/- 0.45 mmol l-1; P less than 0.05), FFA (1.05 +/- 0.08 vs 0.54 +/- 0.04 mmol l-1; P less than 0.01) and LPL (4.6 +/- 0.6 vs 2.6 +/- 0.5 mU ml-1; P less than 0.05) in comparison with LCT administration. There was a positive correlation between plasma LPL activity and TG concentration (r = 0.77; P less than 0.001) when data for the two infusions were combined. Although the same amount of fat was infused on a weight basis, the molar infusion rate was 40% higher with MCT/LCT than with LCT infusion, due to differences in molecular weights (634 vs 885 Da).(ABSTRACT TRUNCATED AT 250 WORDS)

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“…Such a defect would be expected to lead to a decreased release of fatty acid from the tissue. Since the levels and turnover of plasma fatty acids are primarily determined by the release from the adipose organ [6] a reduced beta-receptor activity would be expected to lead to reduced levels of plasma fatty acids. In fact, the opposite usually occurs in adult diabetes, where raised plasma fatty acid levels (and turnover) have been reported [7].…”

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confidence: 99%

Catecholamine receptor sensitivity and the regulation of lipolysis in adult diabetes

Reckless

Galton

1976

Diabetologia

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Summary. The sensitivity of alpha-and beta-adrenergic receptors, and the antilipolytic actions of prostaglandin E 1 or insulin on adipose tissue of obese diabetic and non-diabetic subjects have been studied. Accumulation of cyclic AMP in adipose tissue and release of glycerol in response to several catecholamines (adrenaline, noradrenaline and isoprenaline) in the presence or absence of an alpha-adrenergic blocker (phentolamine) have been used to assess catecholamine receptor sensitivity. No differences in betareceptor activity were observed between diabetics and non-diabetics, either on glycerol release or accumulation of cyclic AMP; alpha-receptor activity was also similar, except for significantly less accumulation of cyclic AMP in diabetic tissue incubated with noradrenaline and phentolamine (p <0.01). The antilipolytic action of prostaglandin E 1 (at concentrations of 30 fM to 30 pM) on lipolysis (stimulated submaximally with isoprenaline, 10 -7 M) was similar in diabetic and control groups. The antilipolytic action of insulin (from 10 -I~ to 10 -6 M) on lipolysis was also similar between the groups. It is concluded that neither disorders of the catecholamine receptor nor of the antilipolytic actions of prostaglandin E 1 or insulin are responsible for the abnormalities of fatty acid metabolism in adult diabetes.

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The effect of a glucose load on plasma fatty acids and lipolysis in adipose tissue of obese diabetic and non-diabetic patients

Cited by 27 publications

References 7 publications

Abnormalities in the adrenergic control and the rate of lipolysis in isolated human subcutaneous adipose tissue in diabetes mellitus

Abnormalities in the adrenergic control and the rate of lipolysis in isolated human subcutaneous adipose tissue in diabetes mellitus

Effect of medium‐ and long‐chain triglyceride infusion on lipoprotein and hepatic lipase in healthy subjects

Catecholamine receptor sensitivity and the regulation of lipolysis in adult diabetes

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