The effect of a lactic acid bacterial inoculant with enzymes on the fermentation dynamics, intake and digestibility of Digitaria eriantha silage

Meeske, R.; Basson, H.M.; Cruywagen, C.W.

doi:10.1016/s0377-8401(99)00089-9

Cited by 49 publications

(60 citation statements)

References 19 publications

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“…Most yeasts can grow within the pH range 3-8 and some strains are able to withstand acidities of pH 2 or below (McDonald et al, 1991). In agreement with our results, when the silage was exposed to air, the yeast counts of control and silage inoculated with L. plantarum, E. faecium and Pediococcus acidilactici increased dramatically (Meeske et al, 1999). In addition, the same authors reported that no mould was detected in the inoculated silage after day 5 of ensiling when the pH was 4.4.…”

Section: Discussionsupporting

confidence: 92%

A new probiotic and bacteriocin-producing strain of Enterococcus faecium EF9296 and its use in grass ensiling

Marciňáková¹,

Lauková²,

Simonová³

et al. 2008

CZECH J ANIM SCI

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A new probiotic, bacteriocin-producing strain of Enterococcus faecium EF9296 (isolate of our laboratory) was used in grass ensiling as an inoculant. The antimicrobial effect as well as the nutritive quality of silage was evaluated along with the aerobic stability test. Grass was ensiled with EF9296 strain (109 colony-forming units per gram of fresh material) and the silage without EF9296 was also prepared. The silages were processed in 1-litre plastic bottles. The samples were collected at days 0–1 (start of experiment), 7, 14 and 105 of ensiling to check the microbial status and chemical parameters of silage. At the end of ensiling period (day 105) the silages were subjected to an aerobic stability test for 7 days. The EF9296 strain survived and colonized the silage in sufficient counts (109–1010 cfu/g). The total counts of enterococci and lactic acid bacteria were increased. In the silage inoculated with EF9296 strain, a reduction in E. coli, enterobacteriae, staphylococci and Bacillus-like bacteria was noted mainly at day 7 and 14 of ensiling. Moreover, the counts of Listeria and moulds were reduced during the aerobic stability test. The silage inoculation resulted in a more rapid drop of pH, higher level of lactic acid and in a decrease in the concentration of acetic and butyric acid. The effect of E. faecium EF9296 on the value of pH was prolonged until the end of the experiment (including the aerobic stability test, below 4.3). During the whole ensiling period an increase in DM content in the experimental silage was detected. The E. faecium EF9296 strain represents a promising silage additive to produce silage of good quality and/or to prevent silage contamination.

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Section: Discussionsupporting

confidence: 92%

A new probiotic and bacteriocin-producing strain of Enterococcus faecium EF9296 and its use in grass ensiling

Marciňáková¹,

Lauková²,

Simonová³

et al. 2008

CZECH J ANIM SCI

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“…This would have resulted in recalcitrant components remaining in the samples. The results are supported by reports that cell wall degrading enzymes reduced fibre content in maize forage at ensiling (Sheperd & Kung, 1996;Meeske et al, 1999;Colombatto et al, 2004). The extent to which this occurs is difficult to measure accurately because ensiling probably solubilizes part of the non-starch polysaccharide fraction, so that polysaccharides that are recovered in NDF and ADF fractions would differ before and after ensiling, as observed by De Vries et al (2012).…”

Section: Discussionsupporting

confidence: 84%

Effects of whey, molasses and exogenous enzymes on the ensiling characteristics, nutrient composition and aerobic stability of maize cobs

Kanengoni¹,

Nkosi²,

Chimonyo³

et al. 2016

SA J. An. Sci.

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The study was conducted to assess the effects of whey, molasses and exogenous enzymes on fermentation, aerobic stability and nutrient composition of ensiled maize cobs. Five treatments were ensiled in 1.5 L anaerobic glass jars over 32 days, namely i) control (maize cobs without additives (CON); ii) maize cobs with sugarcane molasses only (MOL); iii) MOL and whey (MOW); iv) MOW and exogenous enzyme at 0.5 g/kg maize cob mixture (ENZ1) and v) MOW and exogenous enzyme at 1 g/kg maize cob mixture (ENZ2). There was a day effect on gross energy (GE) for CON, MOL, ENZ1 and ENZ2 and none for MOW. There was a day effect in ADF for MOL, ENZ1 and ENZ2 and none for CON and MOW. There was lower dry matter (DM) on day 32 compared with day 0 for all treatments. There were treatment effects for GE, crude protein (CP), ash, ether extract, amylase-treated neutral detergent fibre and acid detergent fibre (ADF). On day 32, the neutral detergent fibre (NDF) and ADF concentrations in CON were higher than in MOL, MOW and ENZ2. At day 32, CON silage pH (4.2) was lower than the pH values of ENZ1 (4.5) and ENZ2 (4.6) silages. Acetic acid levels were higher in ENZ1 than ENZ2 silages. Ammonia nitrogen (NH 3 -N) levels averaged 25 ± 6.6 g NH 3 -N/kg total nitrogen, which is indicative of satisfactory ensiling. The MOL, ENZ1 and ENZ2 silages produced more carbon dioxide than CON and MOW silages. The addition of molasses and whey did not improve the nutritive quality of the maize cobs after ensiling. Ensiling maize cobs with molasses, whey and exogenous enzymes resulted in lower DM and fibre levels in the maize cobs, which could enhance intake and digestibility of nutrients when offered to pigs as a component of a balanced diet. Further investigations using higher concentrations of whey and molasses are warranted to improve the energy and protein composition of the maize cobs. ______________________________________________________________________________________

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“…Those analyses of count were performed in 10 2 cfu/g silage dilution, whose initial microflora was low. Meeske et al (1999) also found a low population of Lactobacillus: nearly 101 cfu/g of fresh forage in Digitaria eriantha plants. Even with a low count, the population showed an increase at opening of silos, probably due to the presence of factors favorable to such growth.…”

Section: Resultsmentioning

confidence: 88%

Microbiological profile and aerobic stability of Tifton 85 bermudagrass silage with different additives

Neres¹,

Zambom²,

Fernandes³

et al. 2013

R. Bras. Zootec.

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-The objective of this study was to evaluate the microbiological profile and aerobic stability of silage with Tifton 85 bermudagrass and different additives and wilting. The studied treatments were: pre-drying in the sun for two hours before ensiling; use of bacterial-enzymatic inoculant; addition of soybean peel; addition of corn grits and use of salt in the surface layer of the silo. The experimental design was completely randomized with six treatments and four replications. Plants of Tifton 85 bermudagrass with 38 days were ensiled in experimental silos with Bunsen valves, with compacting density of 236 kg silage per m 3 for Tifton 85 bermudagrass, which was pre-dried in the sun; the average silage was 294 kg m -3 for the other treatments. The ratios between soybean hulls and corn grits, added to the silage, were calculated based on the initial DM content from Tifton 85 bermudagrass in order to obtain 320 g/kg DM of the material to be ensiled. There was an increase of lactic bacteria and Clostridium as well as an absence of enterobacteria after the silo opening. Fungi developed only in Tifton 85 bermudagrass and its treatments before the ensilage and yeasts developed in silages of Tifton 85 bermudagrass when they received soybean hulls or corn grits after silo opening. There was no breach regarding aerobic stability of silages during the studied period. The pH reached the highest values within 48 hours after opening the silos, but there were variations during this period.

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The effect of a lactic acid bacterial inoculant with enzymes on the fermentation dynamics, intake and digestibility of Digitaria eriantha silage

Cited by 49 publications

References 19 publications

A new probiotic and bacteriocin-producing strain of Enterococcus faecium EF9296 and its use in grass ensiling

A new probiotic and bacteriocin-producing strain of Enterococcus faecium EF9296 and its use in grass ensiling

Effects of whey, molasses and exogenous enzymes on the ensiling characteristics, nutrient composition and aerobic stability of maize cobs

Microbiological profile and aerobic stability of Tifton 85 bermudagrass silage with different additives

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